Mouse PILR-alpha Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PILR-alpha in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse PILR-beta is observed and 5% cross-reactivity with recombinant human PILR-alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse PILR-alpha isoform 1 (R&D Systems, Catalog # 4318-PR)
    Leu21-Val197
    Accession # Q2YFS3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse PILR-alpha (Catalog # 4318-PR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PILR‑ alpha in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse PILR‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4318, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PILR-alpha

PILR-alpha (paired immunoglobulin-like type 2 receptor-alpha; also namedFDF03) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-beta and it likely gave rise to PILR-beta through gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-alpha and -beta should not be confused with the similarly named PIRs (also paired immunoglobulin‑like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig‑like domains in their extracellular region, PILR-alpha and -beta show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-alpha is a monomeric, 271 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a long, 83 aa cytoplasmic region. The ECD shows one V‑type Ig-like domain between aa 39 - 157, while the cytoplasmic region contains two ITIMs (immunoreceptor Tyr-based inhibitory motifs) between aa 265-270 and 294‑299. Given that ITIMs are known to interact with phosphatases such as PTPN6 and PTPN11, the presence of these motifs makes mouse PILR-alpha an inhibitory receptor. In human, activation of PILR-alpha inhibits CD32/Fc gamma RII-induced calcium mobilization (3). Although CD99 is a known ligand for both PILR-alpha and -beta (4), highest affinity binding seems to occur between CD99 and PILR-alpha (4). Mouse PILR-alpha is found on neutrophils and macrophages (4). Mouse PILR-alpha ECD is 43% and 69% aa identical to human and rat PILR-alpha ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-beta (3). One potential isoform of PILR-alpha has been reported. It varies only within the first 28 aa of the signal sequence (5).

  • References:
    1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
    2. Lanier, L.L. (2001) Curr. Opin. Immunol. 13:326.
    3. Fournier, N. et al. (2000) J. Immunol. 165:1197.
    4. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
    5. SwissProt # Q2YFS3.
  • Long Name:
    Paired-Ig-likeType 2 Receptor alpha
  • Entrez Gene IDs:
    29992 (Human); 231805 (Mouse); 288568 (Rat)
  • Alternate Names:
    Cell surface receptor FDF03; FDF03; inhibitory receptor PILRalpha; Inhibitory receptor PILR-alpha; paired immunoglobin-like receptor alpha; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like receptor alpha; paired immunoglobulin-like type 2 receptor alpha; PILRA; PILRalpha; PILR-alpha
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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