Mouse Podoplanin Antibody (3349D)

R&D Systems | Catalog # MAB11813

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, COMET

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 3349D
View all Podoplanin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived mouse Podoplanin
Gln21-Lys133
Accession # Q62011

Specificity

Detects recombinant mouse podoplanin protein in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse Podoplanin Antibody (3349D)

Detection of PDPN in Mouse Thymus via seqIF™ staining on COMET™

PDPN was detected in immersion fixed paraffin-embedded sections of mouse thymus using Rabbit Anti-Mouse PDPN Monoclonal Antibody (Catalog # MAB11813) at 0.1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of PDPN in Mouse Kidney via seqIF™ staining on COMET™

PDPN was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rabbit Anti-Mouse PDPN Monoclonal Antibody (Catalog # MAB11813) at 0.1 μg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of PDPN in Mouse Lung via seqIF™ staining on COMET™

PDPN was detected in immersion fixed paraffin-embedded sections of mouse lung using Rabbit Anti-Mouse PDPN Monoclonal Antibody (Catalog # MAB11813) at 0.1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of PDPN in Mouse Thymus via seqIF™ staining on COMET™

PDPN was detected in immersion fixed paraffin-embedded sections of mouse thymus using Rabbit Anti-Mouse PDPN Monoclonal Antibody (Catalog # MAB11813) at 0.5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Mouse Podoplanin by Western Blot.

Western Blot shows lysates of mouse lung. PVDF membrane was probed with 0.1 µg/ml of Rabbit Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # MAB11813) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Podoplanin at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immersion fixed paraffin-embedded sections of mouse kidney

Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rabbit Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # MAB11813) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Mouse Podoplanin Antibody (3349D)

Application
Recommended Usage

COMET

0.1-0.5 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse thymus, kidney and lung

Immunohistochemistry

0.25-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse kidney

Western Blot

0.1 µg/mL
Sample: Mouse lung tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Podoplanin

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Entrez Gene IDs

10630 (Human); 14726 (Mouse); 54320 (Rat)

Gene Symbol

PDPN

UniProt

Q62011 (Mouse)

Additional Podoplanin Products

Product Documents for Mouse Podoplanin Antibody (3349D)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Podoplanin Antibody (3349D)

For research use only

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Protocols

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