Natural and recombinant mouse Pro-MMP-9. This kit recognizes Pro-MMP-9 when complexed with TIMP-1 but does not recognize active mouse MMP-9.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Mouse Pro-MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure free and TIMP-1 bound mouse Pro-MMP-9 in cell culture supernates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse Pro-MMP-9 and antibodies raised against the recombinant factor. Natural mouse Pro-MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Pro-MMP-9.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
The recovery of mouse Pro-MMP-9 spiked to three levels throughout the range of the assay was evaluated.
Average % Recovery
Cell Culture Samples (n=4)
To assess the linearity of the assay, samples containing high concentrations of mouse Pro-MMP-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat)
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 22.214.171.124; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
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