Mouse Pro-MMP-9 Quantikine ELISA Kit

(15 citations)   
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL)
  • Sensitivity
    14 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
  • Specificity
    Natural and recombinant mouse Pro-MMP-9. This kit recognizes Pro-MMP-9 when complexed with TIMP-1 but does not recognize active mouse MMP-9.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Pro-MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure free and TIMP-1 bound mouse Pro-MMP-9 in cell culture supernates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse Pro-MMP-9 and antibodies raised against the recombinant factor. Natural mouse Pro-MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Pro-MMP-9.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean12620610631332301073
Standard Deviation3.367.5328.61117.555.2
CV%2.73.72.78.37.65.1

Recovery

The recovery of mouse Pro-MMP-9 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 101 82-117
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Pro-MMP-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse Pro-MMP-9 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

    • Long Name
      Matrix Metalloproteinase 9
    • Entrez Gene IDs
      4318 (Human); 17395 (Mouse); 81687 (Rat);
    • Alternate Names
      92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    15 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells
      Authors: A Spiegel, MW Brooks, S Houshyar, F Reinhardt, M Ardolino, E Fessler, MB Chen, JA Krall, J DeCock, IK Zervantona, A Iannello, Y Iwamoto, V Cortez-Ret, RD Kamm, MJ Pittet, DH Raulet, RA Weinberg
      Cancer Discov, 2016;0(0):.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    2. PGI(2) signaling inhibits antigen uptake and increases migration of immature dendritic cells.
      Authors: Toki S, Goleniewska K, Huckabee M, Zhou W, Newcomb D, FitzGerald G, Lawson W, Peebles R
      J Leukoc Biol, 2013;94(1):77-88.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    3. Neutrophil-derived matrix metalloproteinase-9 is a potent activator of trypsinogen in acinar cells in acute pancreatitis.
      Authors: Awla D, Abdulla A, Syk I, Jeppsson B, Regner S, Thorlacius H
      J. Leukoc. Biol., 2012;91(5):711-9.
      Species: Mouse
      Sample Type: Plasma
    4. TIEG1 inhibits breast cancer invasion and metastasis by inhibition of epidermal growth factor receptor (EGFR) transcription and the EGFR signaling pathway.
      Authors: Jin W, Chen BB, Li JY, Zhu H, Huang M, Gu SM, Wang QQ, Chen JY, Yu S, Wu J, Shao ZM
      Mol. Cell. Biol., 2012;32(1):50-63.
      Species: Mouse
      Sample Type: Serum
    5. Matrix metalloproteinase (MMP)-13 regulates mammary tumor-induced osteolysis by activating MMP9 and transforming growth factor-beta signaling at the tumor-bone interface.
      Authors: Nannuru KC, Futakuchi M, Varney ML
      Cancer Res., 2010;70(9):3494-504.
    6. IFN-beta inhibits dendritic cell migration through STAT-1-mediated transcriptional suppression of CCR7 and matrix metalloproteinase 9.
      Authors: Yen JH, Kong W, Ganea D
      J. Immunol., 2010;184(7):3478-86.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    7. Targeting the chemotactic function of CD147 reduces collagen-induced arthritis.
      Authors: Damsker JM, Okwumabua I, Pushkarsky T, Arora K, Bukrinsky MI, Constant SL
      Immunology, 2009;126(1):55-62.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    8. Osteopontin deficiency protects against airway remodeling and hyperresponsiveness in chronic asthma.
      Authors: Simoes DC, Xanthou G, Petrochilou K, Panoutsakopoulou V, Roussos C, Gratziou C
      Am. J. Respir. Crit. Care Med., 2009;179(10):894-902.
      Species: Mouse
      Sample Type: Tissue Homogenates
    9. Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model.
      Authors: Kim DY, Park JW, Jeoung D, Ro JY
      Eur. J. Pharmacol., 2009;612(1):98-105.
      Species: Mouse
      Sample Type: BALF
    10. Retinoic acid decreases adherence of murine myeloid dendritic cells and increases production of matrix metalloproteinase-9.
      Authors: Lackey DE, Ashley SL, Davis AL, Hoag KA
      J. Nutr., 2008;138(8):1512-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. Dopamine regulates endothelial progenitor cell mobilization from mouse bone marrow in tumor vascularization.
      Authors: Chakroborty D, Chowdhury UR, Sarkar C, Baral R, Dasgupta PS, Basu S
      J. Clin. Invest., 2008;118(4):1380-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    12. Functional relevance of the IL-23-IL-17 axis in lungs in vivo.
      Authors: Ivanov S, Bozinovski S, Bossios A, Valadi H, Vlahos R, Malmhall C, Sjostrand M, Kolls JK, Anderson GP, Linden A
      Am. J. Respir. Cell Mol. Biol., 2007;36(4):442-51.
      Species: Mouse
      Sample Type: BALF
    13. Atheroprotective role of interleukin-6 in diet- and/or pathogen-associated atherosclerosis using an ApoE heterozygote murine model.
      Authors: Madan M, Bishayi B, Hoge M, Amar S
      Atherosclerosis, 2007;197(2):504-14.
      Species: Mouse
      Sample Type: Serum
    14. Metalloelastase (MMP-12) induced inflammatory response in mice airways: effects of dexamethasone, rolipram and marimastat.
      Authors: Nenan S, Lagente V, Planquois JM, Hitier S, Berna P, Bertrand CP, Boichot E
      Eur. J. Pharmacol., 2007;559(1):75-81.
      Species: Mouse
      Sample Type: Tissue Homogenates
    15. Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
      Authors: Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP
      Infect. Immun., 2005;73(10):6962-73.
      Species: Mouse
      Sample Type: Tissue Homogenates
    Expand to show all 15 Citations
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