Mouse/Rat alpha 1B-Glycoprotein Antibody

  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat alpha 1B-Glycoprotein in direct ELISAs and Western blots.  In direct ELISAs, less than 1% cross-reactivity with recombinant human  alpha 1B-Glycoprotein observed.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse alpha 1B-Glycoprotein
    Leu21-Gly512
    Accession # Q19LI2
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Simple Western
    1:100 dilution
    See below
  • Immunocytochemistry
    1:20-1:50 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Mouse and Rat
alpha 1B-Glycoprotein by Western Blot.
Western blot shows mouse plasma and rat plasma. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse/Rat alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for alpha
1B-Glycoprotein at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

alpha 1B-Glycoprotein in Hepa 1‑6 Mouse Cell Line. alpha 1B-Glycoprotein was detected in immersion fixed Hepa 1‑6 mouse hepatoma cell line using Rabbit Anti-Mouse/Rat
alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) at 1:20 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse and rat alpha 1B-Glycoprotein by Simple WesternTM. Simple Western lane view shows lysates mouse plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for alpha 1B-Glycoprotein at approximately 113 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Mouse/Rat alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: alpha 1B-Glycoprotein
The alpha 1B-Glycoprotein is a plasma glycoprotein, encoded by the A1BG gene with an unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins.
  • Entrez Gene IDs:
    1 (Human); 117586 (Mouse)
  • Alternate Names:
    A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; GAB
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