Mouse/Rat alpha 1B-Glycoprotein Antibody
Mouse/Rat alpha 1B-Glycoprotein Antibody Summary
Accession # Q19LI2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse and Rat alpha 1B-Glycoprotein by Western Blot. Western blot shows mouse plasma and rat plasma. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse/Rat a 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for a 1B-Glycoprotein at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha 1B-Glycoprotein in Hepa 1‑6 Mouse Cell Line. a 1B-Glycoprotein was detected in immersion fixed Hepa 1-6 mouse hepatoma cell line using Rabbit Anti-Mouse/Rat a 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) at 1:20 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse and rat alpha 1B-Glycoprotein by Simple WesternTM.
Detection of Mouse and rat alpha 1B-Glycoprotein by Simple WesternTM.Simple Western lane view shows lysates mouse plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for alpha 1B-Glycoprotein at approximately 113 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Mouse/Rat alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: alpha 1B-Glycoprotein
The alpha 1B-Glycoprotein is a plasma glycoprotein, encoded by the A1BG gene with an unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins.
Product Specific Notices* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
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