PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70 kDa/CAP70) is a 65‑70 kDa member of the NHER (Na/H Exchange Regulator) family of proteins. It is only expressed in epithelium, particularly that found in intestine, kidney and liver. PDZK1 serves as a scaffold protein that connects cell membrane proteins to cytoplasmic regulators. It also serves a critical role in basal-level bicarbonate secretion. Mouse PDZK1 is 519 amino acids (aa) in length and contains four PDZ domains (aa 9‑90; 128‑215; 243‑323; and 378‑458) plus three phosphorylation sites at Ser489, Ser492 and Ser514. Mouse PDZK1 shares 78% and 91% aa identity with human and rat PDZK1, respectively.
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse PDZK1
aa 1-519
Accession # Q9JIL4
aa 1-519
Accession # Q9JIL4
Specificity
Detects mouse and rat PDZK1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PDZK1 Antibody
Detection of Mouse and Rat PDZK1 by Western Blot.
Western blot shows lysates of mouse liver tissue and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5780) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PDZK1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for PDZK1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse liver tissue and rat liver tissue
Sample: Mouse liver tissue and rat liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PDZK1
Long Name
PDZ Domain Containing 1
Alternate Names
CAP70, CLAMP, NaPi-Cap1, NHERF3, PDZD1
Gene Symbol
PDZK1
UniProt
Additional PDZK1 Products
Product Documents for PDZK1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for PDZK1 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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