Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and recombinant mouse PTP1B 5 ng/lane) were included. A specific band for PTP1B was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 56 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Protein tyrosine phosphatase 1B (PTP1B), also known as protein tyrosine phosphatase, non-receptor type 1 (PTPN1) and placental protein tyrosine phosphatase, dephosphorylates tyrosine residues in proteins. PTP1B is a cytosolic enzyme anchored in the endoplasmic reticulum that plays a critical role in regulating growth factor responsiveness. PTP1B knockout mice show increased resistance to high fat diet-induced obesity and have exaggerated responses to insulin.
Protein Tyrosine Phosphatase 1B
Entrez Gene IDs:
5770 (Human); 19246 (Mouse)
EC 22.214.171.124; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1
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