Mouse/Rat PTP1B Antibody

Catalog #: AF3954
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3954
AF3954-SP

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Detection of Mouse/Rat PTP1B by Western Blot.

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Product Details

Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat PTP1B Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat PTP1B in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human PTP1B is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse PTP1B
Met1-Phe431
Accession # P35821

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.3 µg/mL

See below

Simple Western

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse/Rat PTP1B by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and recombinant mouse PTP1B 5 ng/lane) were included. A specific band for PTP1B was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse PTP1B by Simple Western^TM. Simple Western lane view shows lysates of Neuro-2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 56 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B), also known as protein tyrosine phosphatase, non-receptor type 1 (PTPN1) and placental protein tyrosine phosphatase, dephosphorylates tyrosine residues in proteins. PTP1B is a cytosolic enzyme anchored in the endoplasmic reticulum that plays a critical role in regulating growth factor responsiveness. PTP1B knockout mice show increased resistance to high fat diet-induced obesity and have exaggerated responses to insulin.

Long Name

Protein Tyrosine Phosphatase 1B

Entrez Gene IDs

5770 (Human); 19246 (Mouse)

Alternate Names

EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Rat PTP1B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Hepatic protein-tyrosine phosphatase 1B disruption and pharmacological inhibition attenuate ethanol-induced oxidative stress and ameliorate alcoholic liver disease in mice
Authors: MF Hsu, S Koike, A Mello, LE Nagy, FG Haj
Redox Biol, 2020-07-24;36(0):101658.
Species: Human, Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot
Intranasal Targeting of Hypothalamic PTP1B and TCPTP Reinstates Leptin and Insulin Sensitivity and Promotes Weight Loss in Obesity
Authors: GT Dodd, CE Xirouchaki, M Eramo, CA Mitchell, ZB Andrews, BA Henry, MA Cowley, T Tiganis
Cell Rep, 2019-09-10;28(11):2905-2922.e5.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot
Pathological Oxidation of PTPN12 Underlies ABL1 Phosphorylation in Hereditary Leiomyomatosis and Renal Cell Carcinoma
Authors: Y Xu, P Taylor, J Andrade, B Ueberheide, B Shuch, PM Glazer, RS Bindra, MF Moran, WM Linehan, BG Neel
Cancer Res., 2018-10-08;0(0):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2
Authors: R Tsutsumi, J Harizanova, R Stockert, K Schröder, PIH Bastiaens, BG Neel
Nat Commun, 2017-09-06;8(1):466.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot
Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia
Authors: T Tsunekawa, R Banno, A Mizoguchi, M Sugiyama, T Tominaga, T Onoue, D Hagiwara, Y Ito, S Iwama, M Goto, H Suga, Y Sugimura, H Arima
EBioMedicine, 2017-01-09;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr