Mouse/Rat SOD1/Cu-Zn SOD Antibody

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Detection of Mouse/Rat SOD1/Cu-Zn SOD by Western Blot.
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Product Details
Citations (5)
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Mouse/Rat SOD1/Cu-Zn SOD Antibody Summary

Species Reactivity
Mouse, Rat
Detects endogenous mouse and rat SOD1 in Western blots. In Western blots, this antibody shows no cross‑reacivity with recombinant human SOD2 or SOD3.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse SOD1
Accession # P08228
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat SOD1/Cu-Zn SOD antibody by Western Blot. View Larger

Detection of Mouse/Rat SOD1/Cu-Zn SOD by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3787) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOD1/Cu-Zn SOD at approximately 19 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOD1/Cu-Zn SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the converversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 1, SOD1, also known as Cu/Zn SOD, soluble SOD, and IPO-A, is a soluble, cytoplasmic 16 kDa homodimer. Each SOD1 monomer binds one Cu2+ and Zn2+ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD1 shares 23% and 27% sequence identity with SOD2 and SOD3, respectively. Mouse SOD1 is 97% aa identcal to rat SOD1. Mutations in SOD1 have been suggested to be the cause of familial amyotrophic lateral sclerosis (ALS). The ALS-causing mutations of SOD1 are scattered throughout the protein and provide no clear functional or structural clues to the underlying disease mechanism. The oligomerization hypothesis suggests that mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight aggregates that result in the death of motor neurons. The oxidative damage hypothesis suggests that loss of function mutations in SOD1 result in the intracellular accumulation of the superoxide radical, leading to free radical-mediated damage, the release of cytochrome c, and apoptosis. 

Long Name
Superoxide Dismutase-1
Entrez Gene IDs
6647 (Human); 20655 (Mouse); 24786 (Rat)
Alternate Names
ALS; ALS1; amyotrophic lateral sclerosis 1 (adult); Cu; Cu/Zn superoxide dismutase; CuZn SOD; Cu-Zn SOD; EC; homodimer; hSod1; indophenoloxidase A; Ipo1; IPOA; SOD; SOD, cytosolic; SOD, Soluble; SOD1; superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; Zn superoxide dismutase, EC dismutase, cystolic

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Citations for Mouse/Rat SOD1/Cu-Zn SOD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle
    Authors: H Fujiyoshi, T Egawa, E Kurogi, T Yokokawa, K Kido, T Hayashi
    International Journal of Molecular Sciences, 2022-02-07;23(3):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis
    Authors: C Wu, ME Watts, LL Rubin
    Cell Rep, 2019-01-29;26(5):1143-1156.e5.
  3. Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke
    Evid Based Complement Alternat Med, 2016-11-28;2016(0):1698379.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Adverse effects of reduced oxygen tension on the proliferative capacity of rat kidney and insulin-secreting cell lines involve DNA damage and stress responses.
    Authors: Chen JH, Jones RH, Tarry-Adkins J, Smith NH, Ozanne SE
    Exp. Cell Res., 2008-07-28;314(16):3075-80.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Maternal diet influences DNA damage, aortic telomere length, oxidative stress, and antioxidant defense capacity in rats.
    Authors: Tarry-Adkins JL, Martin-Gronert MS, Chen JH, Cripps RL, Ozanne SE
    FASEB J., 2008-01-29;22(6):2037-44.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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