Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry, Immunoprecipitation, Westen Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all WNK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat WNK1
Ser816-Ser1173
Accession # Q9JIH7

Specificity

Detects mouse and rat WNK1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat WNK1 Antibody

Detection of Mouse/Rat WNK1 antibody by Western Blot.

Detection of Mouse/Rat WNK1 by Western Blot.

Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat WNK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WNK1 at approximately 230 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Mouse/Rat WNK1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: DA3 mouse myeloma cell line

Reviewed Applications

Read 1 review rated 4 using AF2849 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WNK1

With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion.

Long Name

WNK Lysine-deficient Protein Kinase 1

Alternate Names

KDP, PHA2C, PRKWNK1

Entrez Gene IDs

65125 (Human); 232341 (Mouse); 116477 (Rat)

Gene Symbol

WNK1

UniProt

Q9JIH7

Additional WNK1 Products

Product Documents for Mouse/Rat WNK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat WNK1 Antibody

For research use only

Citations for Mouse/Rat WNK1 Antibody

Customer Reviews for Mouse/Rat WNK1 Antibody (1)

4 out of 5
1 Customer Rating
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  • Mouse/Rat WNK1 Antibody
    Name: Anonymous
    Application: Immunofluorescence staining
    Sample Tested: Human Dermal Microvascular Endothelial Cells - fixed with PFA
    Species: Human
    Verified Customer | Posted 12/07/2023
    HDME cells were treated with siCTRL (negative control) and siWNK1. After 72 h transfection, the cells were fixed and stained with anti-WNK1 (green; AF2849, Novus Biologicals) and DAPI (blue). Scale bar, 10 μm.
    Mouse/Rat WNK1 Antibody AF2849
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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