Mouse Ret Antibody

(20 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Ret in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Ret
    Leu29-Arg637 (Phe174Ser)
    Accession # P35546
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Ret Fc Chimera (Catalog # 482-RT)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Ret in Mouse Spinal Cord. Ret was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse Ret Antigen Affinity-purified Polyclonal Antibody (Catalog # AF482) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the ventral horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ret

The GDNF family of neurotrophic factors consititute a new family of factors within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and in the adult animal. The GDNF family members (GDNF, neurturin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha ‑1 ‑ 4). GFR alpha -1, -2, and -4 are the preferred ligand-binding subunits for GDNF, neurturin and persephin, respectively. To date, the preferred ligand for GFR alpha ‑3 has not been identified. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Mouse Ret cDNA encodes a 1115 amino acid (aa) residue transmembrane tyrosine kinase with a 28 aa residue signal peptide, a 609 aa residue cysteine-rich extracellular domain and a 456 aa residue cytoplasmic domain. A cadherin-related sequence is also present in the extracellular domain of Ret. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha  complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha.

  • References:
    1. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
    2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
    3. Carlomagno, F. et al. (1998) Endocrinology, 139:3613.
  • Long Name:
    Proto-oncogene Tyrosine-protein Kinase Receptor Ret
  • Entrez Gene IDs:
    5979 (Human); 19713 (Mouse)
  • Alternate Names:
    CDHF12MTC1; CDHR16; EC 2.7.10; Hirschsprung disease 1; Hirschsprung disease); MEN2A; MEN2B; multiple endocrine neoplasia and medullary thyroid carcinoma 1; ret proto-oncogene; RET transforming sequence; Ret
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain
    Authors: L Urien, S Gaillard, L Lo Re, P Malapert, M Bohic, A Reynders, A Moqrich
    Sci Rep, 2017;7(0):43493.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction
    Authors: NA Gabreski, JK Vaghasia, SS Novakova, NQ McDonald, BA Pierchala
    J. Biol. Chem., 2016;291(31):16249-62.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Uncoupling of molecular maturation from peripheral target innervation in nociceptors expressing a chimeric TrkA/TrkC receptor.
    Authors: Gorokhova S, Gaillard S, Urien L, Malapert P, Legha W, Baronian G, Desvignes J, Alonso S, Moqrich A
    PLoS Genet, 2014;10(2):e1004081.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. CaMKK-CaMK1a, a new post-traumatic signalling pathway induced in mouse somatosensory neurons.
    Authors: Elziere L, Sar C, Venteo S, Bourane S, Puech S, Sonrier C, Boukhadaoui H, Fichard A, Pattyn A, Valmier J, Carroll P, Mechaly I
    PLoS ONE, 2014;9(5):e97736.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2011;14(1):54-61.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos.
    Authors: Kawaguchi J, Nichols J, Gierl MS, Faial T, Smith A
    Development, 2010;137(5):693-704.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  8. Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells.
    Authors: Gossrau G, Thiele J, Konang R, Schmandt T, Brustle O
    Stem Cells, 2007;25(4):939-49.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Vascular-derived artemin: a determinant of vascular sympathetic innervation?
    Authors: Damon DH, Teriele JA, Marko SB
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H266-73.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  10. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation.
    Authors: He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann MC, Dym M
    Stem Cells, 2007;26(1):266-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. Nociceptive tuning by stem cell factor/c-Kit signaling.
    Authors: Milenkovic N, Frahm C, Gassmann M, Griffel C, Erdmann B, Birchmeier C, Lewin GR, Garratt AN
    Neuron, 2007;56(5):893-906.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  12. Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings.
    Authors: Igarashi Y, Aita M, Suzuki A, Nandasena T, Kawano Y, Nozawa-Inoue K, Maeda T
    Neurosci. Lett., 2007;412(3):222-6.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  13. C620R mutation of the murine ret proto-oncogene: loss of function effect in homozygotes and possible gain of function effect in heterozygotes.
    Authors: Yin L, Puliti A, Bonora E, Evangelisti C, Conti V, Tong WM, Medard JJ, Lavoue MF, Forey N, Wang LC, Manie S, Morel G, Raccurt M, Wang ZQ, Romeo G
    Int. J. Cancer, 2007;121(2):292-300.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  14. Hand2 is necessary for terminal differentiation of enteric neurons from crest-derived precursors but not for their migration into the gut or for formation of glia.
    Authors: D'Autréaux F, Morikawa Y, Cserjesi P, Gershon MD
    Development, 2007;134(12):2237-49.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. Ablation of persephin receptor glial cell line-derived neurotrophic factor family receptor alpha4 impairs thyroid calcitonin production in young mice.
    Authors: Lindfors PH, Lindahl M, Rossi J, Saarma M, Airaksinen MS
    Endocrinology, 2006;147(5):2237-44.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  16. Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells.
    Authors: Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ
    Exp. Neurol., 2005;191(1):65-76.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  17. TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons.
    Authors: Tamura S, Morikawa Y, Senba E
    Neuroscience, 2005;130(1):223-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  18. Immortalization of mouse germ line stem cells.
    Authors: Hofmann MC, Braydich-Stolle L, Dettin L, Johnson E, Dym M
    Stem Cells, 2005;23(2):200-10.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  19. Expression and function of GDNF family ligands and receptors in the carotid body.
    Authors: Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM
    Exp. Neurol., 2005;191(0):S68-79.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  20. The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells.
    Authors: Schuetz G, Rosario M, Grimm J, Boeckers TM, Gundelfinger ED, Birchmeier W
    J. Cell Biol., 2004;167(5):945-52.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
Expand to show all 20 Citations
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