Mouse Ret Antibody

Catalog # Availability Size / Price Qty
AF482
AF482-SP
Mouse Ret Antibody in Data
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Product Details
Citations (26)
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Mouse Ret Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ret in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ret
Leu29-Arg637 (Phe174Ser)
Accession # P35546
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Ret Fc Chimera (Catalog # 482-RT)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Ret in Mouse Spinal Cord. View Larger

Ret in Mouse Spinal Cord. Ret was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse Ret Antigen Affinity-purified Polyclonal Antibody (Catalog # AF482) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the ventral horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ret

The GDNF family of neurotrophic factors consititute a new family of factors within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and in the adult animal. The GDNF family members (GDNF, neurturin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha ‑1 ‑ 4). GFR alpha -1, -2, and -4 are the preferred ligand-binding subunits for GDNF, neurturin and persephin, respectively. To date, the preferred ligand for GFR alpha ‑3 has not been identified. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Mouse Ret cDNA encodes a 1115 amino acid (aa) residue transmembrane tyrosine kinase with a 28 aa residue signal peptide, a 609 aa residue cysteine-rich extracellular domain and a 456 aa residue cytoplasmic domain. A cadherin-related sequence is also present in the extracellular domain of Ret. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha  complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha.

References
  1. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  3. Carlomagno, F. et al. (1998) Endocrinology, 139:3613.
Long Name
Proto-oncogene Tyrosine-protein Kinase Receptor Ret
Entrez Gene IDs
5979 (Human); 19713 (Mouse)
Alternate Names
CDHF12MTC1; CDHR16; EC 2.7.10; Hirschsprung disease 1; Hirschsprung disease); MEN2A; MEN2B; multiple endocrine neoplasia and medullary thyroid carcinoma 1; ret proto-oncogene; RET transforming sequence; Ret

Product Datasheets

Citations for Mouse Ret Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. A cell fitness selection model for neuronal survival during development
    Authors: Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend
    Nat Commun, 2019;10(1):4137.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch
    Authors: D Acton, X Ren, S Di Costanz, A Dalet, S Bourane, I Bertocchi, C Eva, M Goulding
    Cell Rep, 2019;28(3):625-639.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis
    Authors: L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast
    Cell Rep, 2019;26(13):3484-3492.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Tumor necrosis factor-? mediated pain hypersensitivity through Ret receptor in resiniferatoxin neuropathy
    Authors: SC Lu, YS Chang, HW Kan, YL Hsieh
    Kaohsiung J. Med. Sci., 2018;34(9):494-502.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats
    Authors: JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma
    Diabetes, 2017;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain
    Authors: L Urien, S Gaillard, L Lo Re, P Malapert, M Bohic, A Reynders, A Moqrich
    Sci Rep, 2017;7(0):43493.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. RET-mediated glial cell line derived neurotrophic factor signaling inhibits mouse prostate development
    Authors: HJ Park, EC Bolton
    Development, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction
    Authors: NA Gabreski, JK Vaghasia, SS Novakova, NQ McDonald, BA Pierchala
    J. Biol. Chem., 2016;291(31):16249-62.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. CaMKK-CaMK1a, a new post-traumatic signalling pathway induced in mouse somatosensory neurons.
    Authors: Elziere L, Sar C, Venteo S, Bourane S, Puech S, Sonrier C, Boukhadaoui H, Fichard A, Pattyn A, Valmier J, Carroll P, Mechaly I
    PLoS ONE, 2014;9(5):e97736.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Uncoupling of molecular maturation from peripheral target innervation in nociceptors expressing a chimeric TrkA/TrkC receptor.
    Authors: Gorokhova S, Gaillard S, Urien L, Malapert P, Legha W, Baronian G, Desvignes J, Alonso S, Moqrich A
    PLoS Genet, 2014;10(2):e1004081.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2011;14(1):54-61.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos.
    Authors: Kawaguchi J, Nichols J, Gierl MS, Faial T, Smith A
    Development, 2010;137(5):693-704.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells.
    Authors: Gossrau G, Thiele J, Konang R, Schmandt T, Brustle O
    Stem Cells, 2007;25(4):939-49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation.
    Authors: He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann MC, Dym M
    Stem Cells, 2007;26(1):266-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. C620R mutation of the murine ret proto-oncogene: loss of function effect in homozygotes and possible gain of function effect in heterozygotes.
    Authors: Yin L, Puliti A, Bonora E, Evangelisti C, Conti V, Tong WM, Medard JJ, Lavoue MF, Forey N, Wang LC, Manie S, Morel G, Raccurt M, Wang ZQ, Romeo G
    Int. J. Cancer, 2007;121(2):292-300.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Nociceptive tuning by stem cell factor/c-Kit signaling.
    Authors: Milenkovic N, Frahm C, Gassmann M, Griffel C, Erdmann B, Birchmeier C, Lewin GR, Garratt AN
    Neuron, 2007;56(5):893-906.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Hand2 is necessary for terminal differentiation of enteric neurons from crest-derived precursors but not for their migration into the gut or for formation of glia.
    Authors: D'Autréaux F, Morikawa Y, Cserjesi P, Gershon MD
    Development, 2007;134(12):2237-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings.
    Authors: Igarashi Y, Aita M, Suzuki A, Nandasena T, Kawano Y, Nozawa-Inoue K, Maeda T
    Neurosci. Lett., 2007;412(3):222-6.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  20. Vascular-derived artemin: a determinant of vascular sympathetic innervation?
    Authors: Damon DH, Teriele JA, Marko SB
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H266-73.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Ablation of persephin receptor glial cell line-derived neurotrophic factor family receptor alpha4 impairs thyroid calcitonin production in young mice.
    Authors: Lindfors PH, Lindahl M, Rossi J, Saarma M, Airaksinen MS
    Endocrinology, 2006;147(5):2237-44.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Immortalization of mouse germ line stem cells.
    Authors: Hofmann MC, Braydich-Stolle L, Dettin L, Johnson E, Dym M
    Stem Cells, 2005;23(2):200-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  23. TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons.
    Authors: Tamura S, Morikawa Y, Senba E
    Neuroscience, 2005;130(1):223-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  24. Expression and function of GDNF family ligands and receptors in the carotid body.
    Authors: Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM
    Exp. Neurol., 2005;191(0):S68-79.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  25. Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells.
    Authors: Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ
    Exp. Neurol., 2005;191(1):65-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  26. The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells.
    Authors: Schuetz G, Rosario M, Grimm J, Boeckers TM, Gundelfinger ED, Birchmeier W
    J. Cell Biol., 2004;167(5):945-52.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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