Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RTVP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse RTVP-1
Ser16-Thr223
Accession # Q9CWG1

Specificity

Detects mouse RTVP-1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse RTVP‑1 Antibody

Detection of Mouse RTVP‑1 antibody by Western Blot.

Detection of Mouse RTVP‑1 by Western Blot.

Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse RTVP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RTVP-1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse RTVP‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Balb/3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RTVP-1

RTVP-1 (related to testes-specific, vespid, and pathogenesis protein 1) can act as both a tumor suppressor and tumor promoter gene. RTVP-1 is a tumor suppressor gene based on its regulation by p53, induction of apoptosis, growth inhibition, and decreased expression in prostate tumors when compared to normal prostate. RTVP-1 is a tumor promoter is based on evidence that overexpression of RTVP-1 decreased the phosphorylation of c-Jun, increased Bcl-2 expression, and increased MMP2 activity. In addition, RTVP-1 is expressed at high levels in glioblastomas while barely detectable in normal brain/astrocytes. Differences in the levels of cellular and extracellular forms as well as different cellular processing of RTVP-1 may account for these differing roles. Additionally, increased RTVP-1 expression is associated with myelomocytic differentiation in macrophages.

Long Name

Related to Testis-specific Vespid and Pathogenesis Protein 1

Alternate Names

GLIPR1, RTVP1

Entrez Gene IDs

11010 (Human); 73690 (Mouse); 299783 (Rat)

Gene Symbol

GLIPR1

UniProt

Q9CWG1

Additional RTVP-1 Products

Product Documents for Mouse RTVP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse RTVP‑1 Antibody

For research use only

Related Research Areas

p53 Pathway

Citations for Mouse RTVP‑1 Antibody

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