Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin I1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin I1

Specificity

Detects mouse Serpin I1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Serpin I1 is observed and less than 5% cross‑reactivity with rhSerpin I2is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Serpin I1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Serpin I1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin I1

Serpin I1 (serine proteinase inhibitor-clade I 1; also protease inhibitor 17 and neuroserpin) is a 52 kDa member of the serpin superfamily of protease inhibitors. It is a component of dense-core secretory granules in neurons of the cerebellum, hippocampus and hypothalamus and following release shows inhibitory activity towards tissue-type plasminogen activator. The mouse Serpin I1 precursor is 410 amino acids (aa) in length. It contains a 16 aa signal sequence plus a reactive center loop that lies between Gly348 and Pro375. Mature mouse Serpin I1 (aa 17‑410) shares 88% and 92% aa identity with human and rat Serpin I1, respectively.

Alternate Names

Neuroserpin, PI12

Entrez Gene IDs

5274 (Human); 20713 (Mouse); 116459 (Rat)

Gene Symbol

SERPINI1

Additional Serpin I1 Products

Product Documents for Mouse Serpin I1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Serpin I1 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

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