Mouse Siglec-F Antibody

Detection of Siglec-F by Flow Cytometry

Generation and characterization of the CD68-rtTA mouse line.a Schematic diagram showing the knock-in strategy of the CD68-rtTA line by homologous recombination. b Genetic lineage tracing strategy by Cre-loxP recombination in CD68+ cells after doxycycline (Dox) treatment. c Schematic showing the experimental design. d Flow cytometric and quantification analyses of the percentage of tdT+ cells among CD45+F4/80+ macrophages (efficiency) and the percentage of SiglecF+ alveolar macrophages (AMs, left) and CD11b+ interstitial macrophages (IMs, right) among tdT+ macrophages from the lungs. Data are presented as the mean ± SEM; n = 5 mice per group. e Flow cytometric and quantification analyses of the percentage of tdT+ cells expressing F4/80 (specificity) in the lungs. Data are presented as the mean ± SEM; n = 5 mice per group. f Flow cytometric and quantification analyses of the percentage of CD45+ monocytes expressing tdT from blood. Data are presented as the mean ± SEM; n = 5 mice per group. g Immunostaining for F4/80, tdT, and SiglecF in lung tissue sections. The boxed region is magnified. Yellow arrowheads indicate labeled macrophages. h Quantitative analysis of the percentage of tdT+ cells among F4/80+ macrophages (efficiency), the percentage of F4/80+ cells among tdT+ cells (specificity), and the percentage of AMs and IMs among labeled tdT+ macrophages from images of immunostained lung sections. Data are presented as the mean ± SEM; n = 5 mice per group. i Cartoon image showing the effective labeling of tissue-resident macrophages (TRMs) in CD68-rtTA;TetO-Cre;R26-tdT mice. Scale bars, 100 µm. Each image is representative of five individual samples. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41421-025-00859-0), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Siglec-F by Immunohistochemistry

Generation and characterization of the CD68-rtTA mouse line.a Schematic diagram showing the knock-in strategy of the CD68-rtTA line by homologous recombination. b Genetic lineage tracing strategy by Cre-loxP recombination in CD68+ cells after doxycycline (Dox) treatment. c Schematic showing the experimental design. d Flow cytometric and quantification analyses of the percentage of tdT+ cells among CD45+F4/80+ macrophages (efficiency) and the percentage of SiglecF+ alveolar macrophages (AMs, left) and CD11b+ interstitial macrophages (IMs, right) among tdT+ macrophages from the lungs. Data are presented as the mean ± SEM; n = 5 mice per group. e Flow cytometric and quantification analyses of the percentage of tdT+ cells expressing F4/80 (specificity) in the lungs. Data are presented as the mean ± SEM; n = 5 mice per group. f Flow cytometric and quantification analyses of the percentage of CD45+ monocytes expressing tdT from blood. Data are presented as the mean ± SEM; n = 5 mice per group. g Immunostaining for F4/80, tdT, and SiglecF in lung tissue sections. The boxed region is magnified. Yellow arrowheads indicate labeled macrophages. h Quantitative analysis of the percentage of tdT+ cells among F4/80+ macrophages (efficiency), the percentage of F4/80+ cells among tdT+ cells (specificity), and the percentage of AMs and IMs among labeled tdT+ macrophages from images of immunostained lung sections. Data are presented as the mean ± SEM; n = 5 mice per group. i Cartoon image showing the effective labeling of tissue-resident macrophages (TRMs) in CD68-rtTA;TetO-Cre;R26-tdT mice. Scale bars, 100 µm. Each image is representative of five individual samples. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41421-025-00859-0), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Siglec-F by Flow Cytometry

MDMs significantly increased following bleomycin-induced injury.a Schematic diagram showing the experimental design. Tam tamoxifen, Bleo bleomycin. b Whole-mount bright field, epifluorescence, and Sirius red images of lungs after bleomycin treatment. The boxed region is magnified. c Immunostaining for tdT, F4/80, and SiglecF in lung tissue sections. The boxed region is magnified. White arrowheads indicate MDMs. d Quantitative analysis of the percentage of F4/80+ macrophages expressing tdT (left) and the percentage of alveolar macrophages among labeled tdT+ macrophages (right) in immunostained lung sections. Data are presented as the mean ± SEM; n = 5 mice per group. e Quantification of the numbers of MDMs (tdT+F4/80+) and Mo-AMs (tdT+SiglecF+) in each 10× field from immunostained lung sections. Data are presented as the mean ± SEM; n = 5 mice per group. f, g Flow cytometric and quantification analyses of the percentage of tdT+ cells among CD45+F4/80+ macrophages (MDMs), the percentage of SiglecF+ alveolar macrophages among tdT+ macrophages (Mo-AMs), and the percentage of CD11b+ interstitial macrophages among tdT+ macrophages (Mo-IMs) from the lungs. Data are presented as the mean ± SEM; n = 5 mice per group. h Cartoon image showing the dynamic changes in the number of recruited macrophages in the lungs after bleomycin-induced injury. MDMs monocyte-derived macrophages, Mo-AMs monocyte-derived alveolar macrophages, Mo-IMs monocyte-derived interstitial macrophages. Scale bars, yellow, 1 mm; white and black, 100 µm. Each image is representative of five individual samples. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41421-025-00859-0), licensed under a CC-BY license. Not internally tested by R&D Systems.