Mouse SLAM/CD150 Antibody Summary
Accession # Q9QUM4
Mouse SLAM/CD150 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of SLAM/CD150 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin then stained with Mouse SLAM/CD150 Monoclonal Antibody (Catalog # MAB4330, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4+ and CD8+ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8+ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).
- Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337.
- Castro, A.G. et al. (1999) J. Immunol. 163:5860.
- Cocks, B.G. et al. (1995) Nature 376:260.
- Wang, N. et al. (2004) J. Exp. Med. 199:1255.
- Hahm, B. et al. (2004) Virology 323:292.
- Nanda, N. et al. (2005) Blood 106:3028.
- Kiel, M.J. et al. (2005) Cell 121:1109.
- Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
- Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
- Aversa, G. et al. (1997) J. Immunol. 158:4036.
- Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
- Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
- Tatsuo, H. et al. (2000) Nature 406:893.
- Hsu, E.C. et al. (2001) Virology 279:9.
Citations for Mouse SLAM/CD150 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Conditional deletion of Ahr alters gene expression profiles in hematopoietic stem cells
Authors: JA Bennett, KP Singh, SL Welle, LA Boule, BP Lawrence, TA Gasiewicz
PLoS ONE, 2018;13(11):e0206407.
Sample Types: Whole Cells
Applications: Flow Cytometry
Identification of a clonally expanding haematopoietic compartment in bone marrow.
Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R
EMBO J, 2013;32(2):219-30.
Sample Types: Whole Tissue
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