Mouse SLAM/CD150 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF4330
Product Details
FAQs
Supplemental Products
Reviews

Mouse SLAM/CD150 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SLAM/CD150 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human SLAM, recombinant mouse (rm) 2B4/Fc, rmCD48, and rmCD84 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150
Thr25-Pro242
Accession # Q9QUM4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SLAM/CD150 (Catalog # 4330-SL)

Mouse SLAM/CD150 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse SLAM/CD150 Antibody (Catalog # MAB4330)

Standard: Recombinant Mouse SLAM/CD150 Protein, CF (Catalog # 4330-SL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2 - 7). It is upregulated on activated B cells and CD4+ and CD8+ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8+ T cells (2, 3, 9 - 11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).

References
  1. Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337.
  2. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
  3. Cocks, B.G. et al. (1995) Nature 376:260. 
  4. Wang, N. et al. (2004) J. Exp. Med. 199:1255. 
  5. Hahm, B. et al. (2004) Virology 323:292. 
  6. Nanda, N. et al. (2005) Blood 106:3028. 
  7. Kiel, M.J. et al. (2005) Cell 121:1109. 
  8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
  9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
  11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  12. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
  13. Tatsuo, H. et al. (2000) Nature 406:893.
  14. Hsu, E.C. et al. (2001) Virology 279:9.
Long Name
Signaling Lymphocytic Activation Molecule
Entrez Gene IDs
6504 (Human); 27218 (Mouse)
Alternate Names
CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse SLAM/CD150 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse SLAM/CD150 Biotinylated Antibody and earn rewards!

Have you used Mouse SLAM/CD150 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review