Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SLAM/CD150 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150
Thr25-Pro242
Accession # Q9QUM4

Specificity

Detects mouse SLAM/CD150 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SLAM is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse SLAM/CD150 Antibody

Detection of SLAM/CD150 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of SLAM/CD150 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca2+ionomycin then stained with Sheep Anti-Mouse SLAM/CD150 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4330, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Applications for Mouse SLAM/CD150 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes treated with PMA and Ca2+ ionomycin

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse SLAM/CD150 (Catalog # 4330-SL)

Reviewed Applications

Read 1 review rated 4 using AF4330 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4+ and CD8+ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8+ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).

References

  1. Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337.
  2. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
  3. Cocks, B.G. et al. (1995) Nature 376:260. 
  4. Wang, N. et al. (2004) J. Exp. Med. 199:1255. 
  5. Hahm, B. et al. (2004) Virology 323:292. 
  6. Nanda, N. et al. (2005) Blood 106:3028. 
  7. Kiel, M.J. et al. (2005) Cell 121:1109. 
  8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
  9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
  11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  12. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
  13. Tatsuo, H. et al. (2000) Nature 406:893.
  14. Hsu, E.C. et al. (2001) Virology 279:9.

Long Name

Signaling Lymphocytic Activation Molecule

Alternate Names

CD150, IPO-3, SLAMF1

Entrez Gene IDs

6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)

Gene Symbol

SLAMF1

UniProt

Q9QUM4

Additional SLAM/CD150 Products

Product Documents for Mouse SLAM/CD150 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse SLAM/CD150 Antibody

For research use only

Citations for Mouse SLAM/CD150 Antibody

Customer Reviews for Mouse SLAM/CD150 Antibody (1)

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  • Mouse SLAM/CD150 Antibody
    Name: Anonymous
    Application: Immunofluorescence - fixed-frozen
    Sample Tested: bone marrow
    Species: Mouse
    Verified Customer | Posted 10/21/2018
    Positivity in Megakaryocytes and few progenitors. acceptable background.
    Whole Femur Section, Fixed-frozen and decalcified
    Mouse SLAM/CD150 Antibody AF4330

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