Mouse ST2/IL-33 R Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse ST2/IL-33 R in ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 245707
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
    Ser27-Arg332
    Accession # P14719
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Mouse ST2/IL-33 R Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse ST2/IL-33 R Antibody (Catalog # MAB10041)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse ST2/IL-33 R Biotinylated Antibody (Catalog # BAF1004)
  • ELISA Standard
     
    Recombinant Mouse ST2/IL-33 R Fc Chimera Protein, CF (Catalog # 1004-MR)
  • Neutralization
    Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 0.5-3 ug/mL in the presence of 1 ng/mL Recombinant Human IL‑33.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ST2/IL-33 R in P815 Mouse Cell Line by Flow Cytometry.

P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).

Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody.

Recombinant Human IL‑33 induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL‑33 is neutralized (green line) by increasing concentrations of Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041). The ND50 is typically 0.5-3 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST2/IL-33 R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4‑7). It binds IL-33, a cytokine that is up‑regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11‑13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2 (9, 12‑14).

  • References:
    1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
    2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
    3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
    4. Schmitz, J. et al. (2005) Immunity 23:479.
    5. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
    6. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
    7. Weinberg, E.O. et al. (2002) Circulation 106:2961.
    8. Sanada S. et al. (2007) J. Clin. Invest. 117:1538
    9. Palmer, G. et al. (2008) Cytokine 42:358.
    10. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
    11. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
    12. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
    13. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
    14. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
  • Long Name:
    Interleukin 33 Receptor
  • Entrez Gene IDs:
    9173 (Human); 17082 (Mouse)
  • Alternate Names:
    DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. CD301b(+) dermal dendritic cells drive T helper 2 cell-mediated immunity.
    Authors: Kumamoto Y, Linehan M, Weinstein J, Laidlaw B, Craft J, Iwasaki A
    Immunity, 2013;39(4):733-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.
    Authors: Monticelli LA, Sonnenberg GF, Abt MC
    Nat. Immunol., 2011;12(11):1045-54.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Paracrine IL-33 Stimulation Enhances Lipopolysaccharide-Mediated Macrophage Activation.
    Authors: Ohno T, Oboki K, Morita H
    PLoS ONE, 2011;6(4):e18404.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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