Mouse ST2/IL-33R Antibody

R&D Systems | Catalog # AF1004

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ST2/IL-33R Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
Ser27-Arg332
Accession # P14719

Specificity

Detects mouse ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ST2/IL-33 R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse ST2/IL-33R Antibody

Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody.

Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody.

Recombinant Human IL-33 (Catalog # 3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND50 is typically 1-6 µg/mL.
Mouse ST2 / IL-33 R ELISA Standard Curve

Mouse ST2 / IL-33 R ELISA Standard Curve

Recombinant Mouse ST2/IL-33R Fc Chimera (Catalog # 1004-MR) was serially diluted and captured by Rat Anti-Mouse ST2/IL-33R Monoclonal Antibody (Catalog # MAB10041) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ST2/IL-33R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of ST2/IL-33R by Immunohistochemistry

Detection of ST2/IL-33R by Immunohistochemistry

IL-33/ST2 signaling mediates mechanical hypersensitivity of INC pain model mice. (A) Experimental protocol denoting time points for the incision, treatment and behavioral observation. (B) Overlaid time courses showing PWT changes in control, INC+Iso IgG and INC+IL-33 neutralizing antibody (IL-33 Ab) groups. (C) Normalized area under the curve (AUC) analysis of each curve in panel B. (D) Overlaid time courses showing PWT changes in control, INC+Iso IgG and INC+ST2 neutralizing antibody (ST2 Ab) groups. (E) Normalized AUC analysis of each curve in panel D. (F) Overlaid time courses showing PWT changes in 4 groups of mice as indicated. (G) Normalized AUC analysis of each curve in panel F. (H) Representative pictures of c-Fos immunostaining in ipsilateral spinal cord dorsal horn of 4 groups of mice. Scale bar represents 100 μm. (I) Summarized data showing c-Fos+ cells per observational field of 4 groups. (J) Representative photos showing wound healing of WT+INC vs. St2-/-+INC group of mice on Day 1, 3, 5&7 after incision. (K) Summary of the width (in mm) of the incision on Day 3, 5&7 as in panel J. The suture was removed 1 day after the incision. **p<0.01 vs. control group. #p<0.05, ##p<0.01 vs. IL-33 Ab/ST2 Ab/WT+INC group as indicated. n=6 mice/group. Two-way ANOVA followed with Tukey's post-hoc test was used for panel B, D, F&K. One-way ANOVA followed with Tukey's post-hoc test was used for panel C, E, G&I. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39267790), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ST2/IL-33R by Western Blot

Detection of ST2/IL-33R by Western Blot

The increased expression of IL-33, ST2, TNF-alpha, and IL-1 beta were retarded by intrathecal injection of SIRT1 agonist SRT1720. (A,B) Application of SRT1720(i.t.) reduced the expression of IL-33 and its receptor ST2 after SNI surgery on day 7. (C,D) Inflammatory cytokines TNF-alpha and IL-1 beta were significantly downregulated by delivery of SRT1720(i.t.) on day 7, n = 4/group, ***p < 0.001 compared with the sham group, ###p < 0.001 compared with SNI+DMSO group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32116550), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ST2/IL-33R by Western Blot

Detection of ST2/IL-33R by Western Blot

Spared nerve injury (SNI) induced mechanical allodynia and enhanced expression of interleukin (IL)-33 and ST2 in dorsal root ganglion (DRG) of rats. (A) SNI induced mechanical allodynia in the ipsilateral but not contralateral hind paw (n = 6), but the sham operation did not induce changes in bilateral hind paw(n = 6). (B,C) Compared with the sham rats, expression of IL-33 and its receptor ST2 increased in L4-L6 DRG since day 1 and persisted till day 14 after SNI (n = 4). *p < 0.05, ***p < 0.001 compared with the sham group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32116550), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse ST2/IL-33R Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse ST2/IL-33 R Fc Chimera (Catalog # 1004-MR)

Neutralization

Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 1-6 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33.

Reviewed Applications

Read 2 reviews rated 2.5 using AF1004 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ST2/IL-33R

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4‑7). It binds IL-33, a cytokine that is up‑regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11‑13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33's ability to signal through transmembrane ST2 (9, 12‑14).

References

  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
  4. Schmitz, J. et al. (2005) Immunity 23:479.
  5. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  6. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  7. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  8. Sanada S. et al. (2007) J. Clin. Invest. 117:1538
  9. Palmer, G. et al. (2008) Cytokine 42:358.
  10. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  11. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  12. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  13. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  14. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Long Name

Interleukin 33 Receptor

Alternate Names

DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1

Entrez Gene IDs

9173 (Human); 17082 (Mouse)

Gene Symbol

IL1RL1

UniProt

P14719

Additional ST2/IL-33R Products

Product Documents for Mouse ST2/IL-33R Antibody

Certificate of Analysis

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Product Specific Notices for Mouse ST2/IL-33R Antibody

For research use only

Related Research Areas

Group 2 ILCs
IL-1 Family

Citations for Mouse ST2/IL-33R Antibody

Customer Reviews for Mouse ST2/IL-33R Antibody (2)

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  • IL-33 protein expression
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 09/08/2025
    treated and untreated mouse lung homogenate, IL-33 protein expression using JESS
    checked the protein expression of IL-33 in mouse lung homogenates
    Mouse ST2/IL-33R Antibody AF1004
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 08/06/2016
    Review for ST2/IL-1 R4 Antibody (AF1004)
    Mouse ST2/IL-33R Antibody AF1004

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