Mouse STAT6 Antibody Summary
Accession # P52633
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse STAT6 by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line untreated (-) or treated (+) with 25 μg Recombinant Mouse IL-4 (Catalog # 404-ML) for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse STAT6 Monoclonal Antibody (Catalog # MAB2169), followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for STAT6 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
STAT family proteins mediate cytokine signaling by acting as signal transducers in the cytoplasm and transcription activators in the nucleus. STAT6 is important for the development of Th2 cells and regulation of gene expression by IL-4 and IL-13.
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