Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody

Catalog # Availability Size / Price Qty
AF3454
AF3454-SP
Product Details
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Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Sulfatase Modifying Factor 2/SUMF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) SUMF2 and less than 5% cross-reactivity with rhSUMF1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sulfatase Modifying Factor 2/SUMF2
Gln34-Leu308
Accession # Q8BPG6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Sulfatase Modifying Factor 2/SUMF2
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Sulfatase Modifying Factor 2/SUMF2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase Modifying Factor 2/SUMF2

Mouse SUMF2 is a 30 kDa member of the sulfatase-modifying factor family. It is synthesized as a 275 amino acid (aa) glycoprotein that is localized to the ER. It contains multiple calcium-binding sites and three (sub)domains of varying length and ill-defined structure (aa 38‑298). There is one potential alternate start site at aa 118 that would generate a 190 aa precursor. The founding family member, SUMF1, posttranslationally activates newly synthesized sulfatases by converting a Cysteine to formylGly in the catalytic site. SUMF2 apparently has no such activity. It forms homodimers and heterodimers with SUMF1. As a heterodimer, it downmodulates SUMF1 activity. Mature mouse SUMF2 is 85% and 93% aa identical to human and rat SUMF2, respectively.

Entrez Gene IDs
25870 (Human)
Alternate Names
C-alpha-formyglycine-generating enzyme 2; C-alpha-formylglycine-generating enzyme 2; DKFZp566I1024; DKFZp686I1024; DKFZp686L17160; DKFZp781L1035; FGE2; MGC99485; paralog of the formylglycine-generating enzyme; pFGE; sulfatase modifying factor 2; sulfatase-modifying factor 2; SUMF2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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