TFPI-2 (tissue factor pathway inhibitor-2; also placental protein 5/PP5) is a secreted, presumably 27‑34 kDa glycoprotein member of the Kunitz domain-containing proteinase inhibitor family. In mouse, it is principally produced by endothelium, hepatocytes and synctiotrophoblasts, and serves as an inhibitor of multiple serine proteinases. Mature mouse TFPI-2 is 208 amino acids (aa) in length. It contains three Kunitz inhibitor domains, the most N-terminal of which (aa 36‑86) is crucial to inhibitor activity. Over aa 23‑211, mouse TFPI-2 shares 86% and 58% aa sequence identity with rat and human TFPI-2, respectively.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TFPI-2
Leu23-Lys211
Accession # O35536
Leu23-Lys211
Accession # O35536
Specificity
Detects mouse TFPI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombiannt human (rh) TFPI-2, rhTFPI-1, and recombinant mouse TFPI-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse TFPI‑2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse TFPI‑2, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse TFPI‑2, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse TFPI‑2
Sample: Recombinant Mouse TFPI‑2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TFPI-2
Long Name
Tissue Factor Pathway Inhibitor 2
Alternate Names
PP5, REF-1, TFPI2
Entrez Gene IDs
7980 (Human)
Gene Symbol
TFPI2
UniProt
Additional TFPI-2 Products
Product Documents for Mouse TFPI‑2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse TFPI‑2 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
