Mouse THRSP Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse THRSP in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse THRSP
    Gly47-Leu150
    Accession # Q62264
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Mouse THRSP by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse THRSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for THRSP at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: THRSP

THRSP (Thyroid hormone inducible hepatic protein; also S14/SPOT 14) is an 18-20 kDa acidic nuclear protein that belongs to the SPOT14 family of molecules. It is expressed by lactating mammary epithelium, adipocytes and hepatocytes, and appears to promote the synthesis of long-chain fatty acids. Its synthesis is induced by thyroid hormone, insulin and carbohydrates. Mouse THRSP is 150 amino acids (aa) in length. The residues between aa 10-66 contain a Leu-zipper domain that mediates noncovalent homodimerization plus heterodimerization with MIG12. Other proteins known to bind to THRSP include the thyroid hormone receptor and Zac51. Heterointeractions are posited to positively influence p53-dependent gene activation. Two potential isoforms are reported. One contains a seven aa extension at the C-terminus, while another shows a deletion of aa 31-64. Over aa 47-150, mouse THRSP shares 78% and 94% aa identity with human and rat THRSP, respectively.

  • Long Name:
    Thyroid Hormone Responsive [SPOT14 Homolog] Protein
  • Entrez Gene IDs:
    7069 (Human); 21835 (Mouse); 25357 (Rat)
  • Alternate Names:
    lipogenic protein 1; Lpgp; LPGP1; S14; S14MGC21659; Spot 14 protein; SPOT14; SPOT14SPOT14 homolog; THRSP; thyroid hormone responsive (SPOT14 homolog, rat); thyroid hormone responsive SPOT14 (rat) homolog; thyroid hormone responsive SPOT14; thyroid hormone responsive; thyroid hormone-inducible hepatic protein
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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 203  
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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