Mouse THRSP Antibody

Catalog # Availability Size / Price Qty
AF6574
AF6574-SP
Mouse THRSP Antibody in Western Blot
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Mouse THRSP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse THRSP in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse THRSP
Gly47-Leu150
Accession # Q62264
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse THRSP by Western Blot. View Larger

Detection of Mouse THRSP by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse THRSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for THRSP at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THRSP

THRSP (Thyroid hormone inducible hepatic protein; also S14/SPOT 14) is an 18-20 kDa acidic nuclear protein that belongs to the SPOT14 family of molecules. It is expressed by lactating mammary epithelium, adipocytes and hepatocytes, and appears to promote the synthesis of long-chain fatty acids. Its synthesis is induced by thyroid hormone, insulin and carbohydrates. Mouse THRSP is 150 amino acids (aa) in length. The residues between aa 10-66 contain a Leu-zipper domain that mediates noncovalent homodimerization plus heterodimerization with MIG12. Other proteins known to bind to THRSP include the thyroid hormone receptor and Zac51. Heterointeractions are posited to positively influence p53-dependent gene activation. Two potential isoforms are reported. One contains a seven aa extension at the C-terminus, while another shows a deletion of aa 31-64. Over aa 47-150, mouse THRSP shares 78% and 94% aa identity with human and rat THRSP, respectively.

Long Name
Thyroid Hormone Responsive [SPOT14 Homolog] Protein
Entrez Gene IDs
7069 (Human); 21835 (Mouse); 25357 (Rat)
Alternate Names
lipogenic protein 1; Lpgp; LPGP1; S14; S14MGC21659; Spot 14 protein; SPOT14; SPOT14SPOT14 homolog; THRSP; thyroid hormone responsive (SPOT14 homolog, rat); thyroid hormone responsive SPOT14 (rat) homolog; thyroid hormone responsive SPOT14; thyroid hormone responsive; thyroid hormone-inducible hepatic protein

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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