Mouse TIM-1/KIM-1/HAVCR Antibody

Catalog # Availability Size / Price Qty
MAB1817-SP
MAB1817-500
MAB1817-100
Product Details
Citations (9)
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Mouse TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIM‑1/KIM-1/HAVCR in ELISAs and Western blots. This antibody does not cross-react with recombinant human (rh) TIM‑1, rmTIM-2, or rmTIM-3.
Source
Monoclonal Rat IgG2B Clone # 222414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
Tyr22-Thr212
Accession # NP_001160104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TIM‑1/KIM‑1/HAVCR (Catalog # 1817-TM)

Mouse TIM-1/KIM-1/HAVCR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TIM‑1/KIM‑1/HAVCR Biotinylated Antibody (Catalog # BAF1817)

Standard: Recombinant Mouse TIM-1/KIM-1/HAVCR Protein (Catalog # 1817-TM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 or HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39% and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

References
  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. 
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
  4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.  
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113. 
  6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  8. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

Product Datasheets

Citations for Mouse TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Dietary sodium modulates nephropathy in Nedd4-2-deficient mice
    Authors: JA Manning, SS Shah, TL Henshall, A Nikolic, J Finnie, S Kumar
    Cell Death Differ., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury
    Authors: A Lau, H Chung, T Komada, JM Platnich, CF Sandall, SR Choudhury, J Chun, V Naumenko, BG Surewaard, MC Nelson, A Ulke-Lemée, PL Beck, H Benediktss, AM Jevnikar, SL Snelgrove, MJ Hickey, DL Senger, MT James, JA Macdonald, P Kubes, CN Jenne, DA Muruve
    J. Clin. Invest., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. The pathological role of IL-18Ralpha in renal ischemia/reperfusion injury.
    Authors: Yano T, Nozaki Y, Kinoshita K, Hino S, Hirooka Y, Niki K, Shimazu H, Kishimoto K, Funauch M, Matsumura I
    Lab Invest, 2015;95(1):78-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. KIM-1-mediated phagocytosis reduces acute injury to the kidney.
    Authors: Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J
    J Clin Invest, 2015;125(4):1620-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development (Capture)
  5. Proximal tubule-derived colony stimulating factor-1 mediates polarization of renal macrophages and dendritic cells, and recovery in acute kidney injury.
    Authors: Wang Y, Chang J, Yao B, Niu A, Kelly E, Breeggemann M, Abboud Werner S, Harris R, Zhang M
    Kidney Int, 2015;88(6):1274-1282.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. HAVCR/KIM-1 activates the IL-6/STAT-3 pathway in clear cell renal cell carcinoma and determines tumor progression and patient outcome.
    Authors: Cuadros T, Trilla E, Sarro E, Vila M, Vilardell J, de Torres I, Salcedo M, Lopez-Hellin J, Sanchez A, Ramon y Cajal S, Itarte E, Morote J, Meseguer A
    Cancer Res, 2014;74(5):1416-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Endogenous Tim-1 (Kim-1) promotes T-cell responses and cell-mediated injury in experimental crescentic glomerulonephritis.
    Authors: Nozaki Y, Nikolic-Paterson DJ, Snelgrove SL
    Kidney Int., 2012;81(9):844-55.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. T cell Ig domain and mucin domain 1 engagement on invariant NKT cells in the presence of TCR stimulation enhances IL-4 production but inhibits IFN-gamma production.
    Authors: Kim HS, Kim HS, Lee CW, Chung DH
    J. Immunol., 2010;184(8):4095-106.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
    Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
    Blood, 2007;110(7):2565-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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