Tryptase-5 is encoded by Prss32, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse Tryptase-5 consists of 331 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 53), a catalytic domain (54 to 304), and a C-terminal hydrophobic peptide (residues 305 to 331). The mRNA is expressed in smooth muscle, eye, stomach, uterus and lymph node. Apparently, Prss32 does not seem to have a counterpart in the human genome.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Neutralization, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Tryptase‑5/Prss32
Ser20-Arg304
Accession # NP_081496
Ser20-Arg304
Accession # NP_081496
Specificity
Detects mouse Tryptase‑5/Prss32 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Tryptase-G1, recombinant mouse (rm) MCPT-6, and rmMCPT‑7 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse Tryptase‑5/Prss32 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Tryptase‑5/Prss32 (Catalog # 2634-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Tryptase‑5/Prss32 (Catalog # 2634-SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Tryptase‑5/Prss32 (Catalog # 2634-SE)
Sample: Recombinant Mouse Tryptase‑5/Prss32 (Catalog # 2634-SE)
Neutralization
Measured by its ability to neutralize Recombinant Mouse Tryptase‑5/Prss32 (2 µg/mL, Catalog # 2634-SE) cleavage of the fluorogenic peptide substrate Z-R-SBzl (100 µM). The Neutralization Dose (ND50) is typically 24.3 µg/mL.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Tryptase-5/Prss32
References
- Wong, G. W. et al. (2004) J. Biol. Chem. 279:2438.
Alternate Names
Prss32, Tryptase5
Entrez Gene IDs
69814 (Mouse)
Gene Symbol
PRSS32
UniProt
Additional Tryptase-5/Prss32 Products
Product Documents for Mouse Tryptase‑5/Prss32 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Tryptase‑5/Prss32 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars