Mouse VEGF164 Antibody

(61 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse VEGF in ELISAs and Western blots. In Western blots, detection of recombinant mouse (rm) VEGF164, rmVEGF120, rmVEGF188, and recombinant rat VEGF164 is observered but no cross-reactivity with rmVEGF-B167,rmVEGF-B186 , or recombinant human (rh) VEGF165 is observed. In sandwich immunoassays, less than 0.04% cross-reactivity with rhVEGF121, rhVEGF165, and rhVEGF/PlGF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF164
    Ala27-Arg190
    Accession # AAA40547
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse VEGF Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse VEGF164 Antibody (Catalog # AF-493-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse VEGF Biotinylated Antibody (Catalog # BAF493)
  • ELISA Standard
     
    Recombinant Mouse VEGF 164 Protein (Catalog # 493-MV)
  • Neutralization
    Measured by its ability to neutralize VEGF164-induced proliferation in HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci USA 87:1323. The Neutralization Dose (ND50) is typically 0.05‑0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse VEGF164.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human, Mouse, and Rat VEGF by Western Blot. Western blot shows 25 ng of Recombinant Human VEGF165 (Catalog # 293-VE), Recombinant Mouse VEGF164 (Catalog # 493-MV), Recombinant Mouse VEGF120 (Catalog # 494-VE), Recombinant Mouse VEGF‑B167 (Catalog # 2595-VE), Recombinant Mouse VEGF‑B 186 (Catalog # 767-VE), Recombinant Mouse VEGF188 (Catalog # 7916-MV), and Recombinant Rat VEGF164 (Catalog # 564-RV). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-493-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for VEGF at approximately 18-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
VEGF164 in Mouse Kidney. Vascular Endothelial Growth Factor 164 (VEGF164) was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Cell Proliferation Induced by VEGF164 and Neutralization by Mouse VEGF Antibody. Recombinant Mouse VEGF164 (Catalog # 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF164 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA). The ND50 is typically 0.05-0.15 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues (4). Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in mouse (2, 4). Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Mouse VEGF164 shares 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 88% with bovine, and 90% with feline, equine and canine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). Human VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9).

  • References:
    1. Breier, G. et al. (1992) Development 114:521.
    2. Shima, D.T. et al. (1996) J. Biol. Chem. 271:3877.
    3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
    4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
    5. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
    6. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.
    7. Thurston, G. (2002) J. Anat. 200:575.
    8. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.
    9. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.
  • Long Name:
    Vascular Endothelial Growth Factor
  • Entrez Gene IDs:
    7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
  • Alternate Names:
    MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

61 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Modulation of Salmonella Tumor-Colonization and Intratumoral Anti-angiogenesis by Triptolide and Its Mechanism
    Authors: J Chen, Y Qiao, B Tang, G Chen, X Liu, B Yang, J Wei, X Zhang, X Cheng, P Du, W Jiang, Q Hu, ZC Hua
    Theranostics, 2017;7(8):2250-2260.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Control of metastatic niche formation by targeting APBA3/Mint3 in inflammatory monocytes
    Authors: T Hara, HJ Nakaoka, T Hayashi, K Mimura, D Hoshino, M Inoue, F Nagamura, Y Murakami, M Seiki, T Sakamoto
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis
    Authors: XW Chen, TJ Yu, J Zhang, Y Li, HL Chen, GF Yang, W Yu, YZ Liu, XX Liu, CF Duan, HL Tang, M Qiu, CL Wang, H Zheng, J Yue, AM Guo, J Yang
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Functional Assay
  4. Ferrochelatase is a therapeutic target for ocular neovascularization
    Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
    EMBO Mol Med, 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Functional Assay
  5. VEGF as a Paracrine Regulator of Conventional Outflow Facility
    Authors: E Reina-Torr, JC Wen, KC Liu, G Li, JM Sherwood, JY Chang, P Challa, CM Flügel-Koc, WD Stamer, RR Allingham, DR Overby
    Invest. Ophthalmol. Vis. Sci., 2017;58(3):1899-1908.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro
    Sci Rep, 2016;6(0):31572.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  7. Retinal pigment epithelial cell expression of active Rap 1a by scAAV2 inhibits choroidal neovascularization
    Mol Ther Methods Clin Dev, 2016;3(0):16056.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. A novel small molecule ameliorates ocular neovascularisation and synergises with anti-VEGF therapy
    Sci Rep, 2016;6(0):25509.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  9. Antihypoxic Potentiation of Standard Therapy for Experimental Colorectal Liver Metastasis through Myo-Inositol Trispyrophosphate
    Clin Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
    Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
    Oncotarget, 2016;7(47):77902-77915.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  11. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
    Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
    Sci Rep, 2016;6(0):19404.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. The biomechanical properties of an epithelial tissue determine the location of its vasculature
    Nat Commun, 2016;7(0):13560.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  13. HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1alpha activation.
    Authors: Park S, Lee S, Kim H, Lee W, Hong K, Kim C
    Eur J Immunol, 2015;45(4):1216-27.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  14. Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.
    Authors: Sitaras N, Rivera J, Noueihed B, Bien-Aime M, Zaniolo K, Omri S, Hamel D, Zhu T, Hardy P, Sapieha P, Joyal J, Chemtob S
    Am J Pathol, 2015;185(2):581-95.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Neut
  15. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2015;515(7525):130-3.
    Species: Mouse
    Sample Type: In Vivo
  16. MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
    Authors: Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y
    Gene Ther, 2014;21(1):37-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  17. Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta) in the brain.
    Authors: Yu S, Levi L, Casadesus G, Kunos G, Noy N
    J Biol Chem, 2014;289(18):12748-58.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  18. Angiopoietin-1 suppresses choroidal neovascularization and vascular leakage.
    Authors: Lee, Junyeop, Park, Dae-Youn, Park, Do Young, Park, Intae, Chang, Woohyok, Nakaoka, Yoshikaz, Komuro, Issei, Yoo, Ook-Joon, Koh, Gou Youn
    Invest Ophthalmol Vis Sci, 2014;55(4):2191-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  19. IKK2 inhibition attenuates laser-induced choroidal neovascularization.
    Authors: Lu H, Lu Q, Gaddipati S, Kasetti R, Wang W, Pasparakis M, Kaplan H, Li Q
    PLoS ONE, 2014;9(1):e87530.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  20. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
    Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
    Nat Commun, 2014;5(0):4715.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  21. Shiga toxin promotes podocyte injury in experimental hemolytic uremic syndrome via activation of the alternative pathway of complement.
    Authors: Locatelli M, Buelli S, Pezzotta A, Corna D, Perico L, Tomasoni S, Rottoli D, Rizzo P, Conti D, Thurman J, Remuzzi G, Zoja C, Morigi M
    J Am Soc Nephrol, 2014;25(8):1786-98.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  22. Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
    Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
    Exp Cell Res, 2014;323(1):7-27.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA detection
  23. Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
    Authors: Hendel, Alon, Hsu, Ivy, Granville, David J
    Lab Invest, 2014;94(7):716-25.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  24. RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages.
    Authors: Lee Y, Park H, Woo S, Park E, Kang J
    J Pharmacol Exp Ther, 2014;350(3):563-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  25. IL-18 regulates melanoma VLA-4 integrin activation through a Hierarchized sequence of inflammatory factors.
    Authors: Valcarcel M, Carrascal T, Crende O, Vidal-Vanaclocha F
    J Invest Dermatol, 2014;134(2):470-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  26. An inducible hepatocellular carcinoma model for preclinical evaluation of antiangiogenic therapy in adult mice.
    Authors: Runge A, Hu J, Wieland M, Bergeest J, Mogler C, Neumann A, Geraud C, Arnold B, Rohr K, Komljenovic D, Schirmacher P, Goerdt S, Augustin H
    Cancer Res, 2014;74(15):4157-69.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  27. Neutrophils contribute to inflammatory lymphangiogenesis by increasing VEGF-A bioavailability and secreting VEGF-D.
    Authors: Tan K, Chong S, Wong F, Evrard M, Tan S, Keeble J, Kemeny D, Ng L, Abastado J, Angeli V
    Blood, 2013;122(22):3666-77.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  28. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  29. The role of IL-1beta in the early tumor cell-induced angiogenic response.
    Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E
    J Immunol, 2013;190(7):3500-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  30. Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways.
    Authors: Di Bernardini E, Campagnolo P, Margariti A, Zampetaki A, Karamariti E, Hu Y, Xu Q
    J Biol Chem, 2013;289(6):3383-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  31. Folic acid mitigates angiotensin-II-induced blood pressure and renal remodeling.
    Authors: Pushpakumar, Sathnur, Kundu, Sourav, Metreveli, Naira, Sen, Utpal
    PLoS ONE, 2013;8(12):e83813.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  32. Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
    Blood, 2012;119(26):6382-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  33. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  34. IL-17 and VEGF are necessary for efficient corneal nerve regeneration.
    Authors: Li Z, Burns AR, Han L, Rumbaut RE, Smith CW
    Am. J. Pathol., 2011;178(3):1106-16.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  35. Serotonin reverts age-related capillarization and failure of regeneration in the liver through a VEGF-dependent pathway.
    Authors: Furrer K, Rickenbacher A, Tian Y, Jochum W, Bittermann AG, Kach A, Humar B, Graf R, Moritz W, Clavien PA
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(7):2945-50.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  36. Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization.
    Authors: Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS
    J. Biol. Chem., 2011;286(23):20991-1001.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  37. Platelet-derived growth factor-B normalizes micromorphology and vessel function in vascular endothelial growth factor-A-induced squamous cell carcinomas.
    Authors: Lederle W, Linde N, Heusel J, Bzyl J, Woenne EC, Zwick S, Skobe M, Kiessling F, Fusenig NE, Mueller MM
    Am. J. Pathol., 2010;176(2):981-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  38. VEGF-A expression by HSV-1-infected cells drives corneal lymphangiogenesis.
    Authors: Wuest TR, Carr DJ
    J. Exp. Med., 2010;207(1):101-15, S1-2.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  39. CXCR4 blockade augments bone marrow progenitor cell recruitment to the neovasculature and reduces mortality after myocardial infarction.
    Authors: Jujo K, Hamada H, Iwakura A, Thorne T, Sekiguchi H, Clarke T, Ito A, Misener S, Tanaka T, Klyachko E, Kobayashi K, Tongers J, Roncalli J, Tsurumi Y, Hagiwara N, Losordo DW
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(24):11008-13.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  40. Effects of increased renal tubular vascular endothelial growth factor (VEGF) on fibrosis, cyst formation, and glomerular disease.
    Authors: Hakroush S, Moeller MJ, Theilig F, Kaissling B, Sijmonsma TP, Jugold M, Akeson AL, Traykova-Brauch M, Hosser H, Hahnel B, Grone HJ, Koesters R, Kriz W
    Am. J. Pathol., 2009;175(5):1883-95.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  41. Recapitulation of pancreatic neuroendocrine tumors in human multiple endocrine neoplasia type I syndrome via Pdx1-directed inactivation of Men1.
    Authors: Shen HC, He M, Powell A, Adem A, Lorang D, Heller C, Grover AC, Ylaya K, Hewitt SM, Marx SJ, Spiegel AM, Libutti SK
    Cancer Res., 2009;69(5):1858-66.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  42. Critical role of CD11b+ macrophages and VEGF in inflammatory lymphangiogenesis, antigen clearance, and inflammation resolution.
    Authors: Kataru RP, Jung K, Jang C, Yang H, Schwendener RA, Baik JE, Han SH, Alitalo K, Koh GY
    Blood, 2009;113(22):5650-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  43. Safety profile of topical VEGF neutralization at the cornea.
    Authors: Bock F, Onderka J, Rummelt C, Dietrich T, Bachmann B, Kruse FE, Schlotzer-Schrehardt U, Cursiefen C
    Invest. Ophthalmol. Vis. Sci., 2009;50(5):2095-102.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Blocking
  44. Role of cell and matrix-bound VEGF isoforms in lens development.
    Authors: Saint-Geniez M, Kurihara T, D'Amore PA
    Invest. Ophthalmol. Vis. Sci., 2009;50(1):311-21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  45. Fibroblast-type reticular stromal cells regulate the lymph node vasculature.
    Authors: Chyou S, Ekland EH, Carpenter AC, Tzeng TC, Tian S, Michaud M, Madri JA, Lu TT
    J. Immunol., 2008;181(6):3887-96.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  46. Regulation of scar formation by vascular endothelial growth factor.
    Authors: Wilgus TA, Ferreira AM, Oberyszyn TM, Bergdall VK, DiPietro LA
    Lab. Invest., 2008;88(6):579-90.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  47. Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
    Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
    J. Biol. Chem., 2008;283(49):34250-9.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: ELISA Development
  48. Loss of vascular endothelial growth factor expression reduces vascularization, but not growth, of tumors lacking the Von Hippel-Lindau tumor suppressor gene.
    Authors: Blouw B, Haase VH, Song H, Bergers G, Johnson RS
    Oncogene, 2007;26(31):4531-40.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  49. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
    Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
    Am. J. Pathol., 2006;169(5):1767-83.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  50. Mast cells facilitate local VEGF release as an early event in the pathogenesis of postoperative peritoneal adhesions.
    Authors: Cahill RA, Wang JH, Soohkai S, Redmond HP
    Surgery, 2006;140(1):108-12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  51. Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A.
    Authors: Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J
    J. Clin. Invest., 2006;116(2):422-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  52. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
    Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
    Nat. Neurosci., 2006;9(3):340-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  53. Vascular endothelial growth factor-A mediates ultraviolet B-induced impairment of lymphatic vessel function.
    Authors: Kajiya K, Hirakawa S, Detmar M
    Am. J. Pathol., 2006;169(4):1496-503.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  54. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
    Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
    Genes Dev., 2006;20(18):2527-38.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  55. Application of plasmid DNA encoding IL-18 diminishes development of herpetic stromal keratitis by antiangiogenic effects.
    Authors: Kim B, Lee S, Suvas S, Rouse BT
    J. Immunol., 2005;175(1):509-16.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ELISA Development
  56. Vascular endothelial growth factor (VEGF) gene transfer enhances surgical revascularization of necrotic bone.
    Authors: Katsube K, Bishop AT, Simari RD, Yla-Herttuala S, Friedrich PF
    J. Orthop. Res., 2005;23(2):469-74.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC
  57. Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation.
    Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP
    J. Immunol., 2005;174(1):215-22.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Neut
  58. CXCR2-/- mice show enhanced susceptibility to herpetic stromal keratitis: a role for IL-6-induced neovascularization.
    Authors: Banerjee K, Biswas PS, Kim B, Lee S, Rouse BT
    J. Immunol., 2004;172(2):1237-45.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  59. Estrogen regulates the production of VEGF for osteoclast formation and activity in op/op mice.
    Authors: Kodama I, Sanada M, Yoshiko Y, Tsuda M, Ohama K
    J. Bone Miner. Res., 2004;19(2):200-6.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  60. Lack of alpha2-antiplasmin promotes pulmonary heart failure via overrelease of VEGF after acute myocardial infarction.
    Authors: Kozawa O, Yoshimi N, Akamatsu S, Hara A, Mori H, Uematsu T
    Blood, 2002;100(7):2487-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  61. Antibodies to vascular endothelial growth factor enhance the efficacy of cancer immunotherapy by improving endogenous dendritic cell function.
    Authors: Gabrilovich DI, Ishida T, Nadaf S, Ohm JE, Carbone DP
    Clin. Cancer Res., 1999;5(10):2963-70.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Expand to show all 61 Citations

FAQs

  1. What band size(s) are detected when using AF-493-NA for Western blot?

    • This antibody detects Recombinant Mouse VEGF164 at 42 kDa under non-reducing conditions and 21 kDa under reducing conditions.  Band sizes with natural samples may vary slightly depending on any post-translational modifications.  

View all Antibody FAQs
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse VEGF164 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information