Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rabbit, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Blocking, ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), In vivo assay, Functional Assay, Luminex Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VEGF Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF164
Ala27-Arg190
Accession # AAA40547

Specificity

Detects mouse VEGF in ELISAs and Western blots. In Western blots, detection of recombinant mouse (rm) VEGF

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse VEGF164 Antibody

Detection of Recombinant Human, Mouse, and Rat VEGF antibody by Western Blot.

Detection of Recombinant Human, Mouse, and Rat VEGF by Western Blot.

Western blot shows 25 ng of Recombinant Human VEGF165(Catalog # 293-VE), Recombinant Mouse VEGF164(Catalog # 493-MV), Recombinant Mouse VEGF120(Catalog # 494-VE), Recombinant Mouse VEGF-B167(Catalog # 2595-VE), Recombinant Mouse VEGF-B 186 (Catalog # 767-VE), Recombinant Mouse VEGF188(Catalog # 7916-MV), and Recombinant Rat VEGF164(Catalog # 564-RV). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse VEGF164Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for VEGF at approximately 18-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
VEGF164in Mouse Kidney.

VEGF164in Mouse Kidney.

Vascular Endothelial Growth Factor 164 (VEGF164) was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse VEGF164Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by VEGF164 and Neutralization by Mouse VEGF Antibody.

Recombinant Mouse VEGF164 (Catalog # 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by Recombinant Mouse VEGF164 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA). The ND50 is typically
Detection of Mouse VEGF by Immunocytochemistry/Immunofluorescence

Detection of Mouse VEGF by Immunocytochemistry/Immunofluorescence

Altered expression of VEGF-A following myeloid-specific ablation of NG2. Double immunostaining for VEGF-A (red) and CD31 (green) was used to quantify and localize VEGF-A expression in tumors from control (A–C) and Mac-NG2ko tumors (D–F). Overall expression of VEGF-A increases 3-fold in Mac-NG2ko tumors (G), consistent with the increased HIF-1 alpha expression seen in Fig. 5I. However, VEGF-A in control tumors is highly localized to blood vessels, while vascular VEGF-A in Mac-NG2ko tumors is reduced 3-fold (H). Instead, non-vascular VEGF-A in Mac-NG2ko tumors is increased by a factor of 5 (I). Scale bars = 60 μm. *p < 0.01 compared to controls. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26137396), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse VEGF164 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse VEGF164 (Catalog # 493-MV)

Neutralization

Measured by its ability to neutralize VEGF164-induced proliferation in HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci USA 87:1323. The Neutralization Dose (ND50) is typically <0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse VEGF164.

Mouse VEGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse VEGF Biotinylated Antibody (Catalog # BAF493)
  • Standard: Recombinant Mouse VEGF 164 Protein (Catalog # 493-MV)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4 using AF-493-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Mouse VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 90% with feline, equine and canine, and 88% with bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

AAA40547

Additional VEGF Products

Product Documents for Mouse VEGF164 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse VEGF164 Antibody

For research use only

Citations for Mouse VEGF164 Antibody

Customer Reviews for Mouse VEGF164 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
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  • Mouse VEGF164 Antibody
    Name: Leslie Priddy
    Application: Western Blot
    Sample Tested: Tumor cell lyastes
    Species: Mouse
    Verified Customer | Posted 04/01/2018
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23670941
    Species: Mouse
    Verified Customer | Posted 01/08/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Primary mouse cortical astrocytes
    Species: Mouse
    Verified Customer | Posted 12/19/2014

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mouse VEGF164 Antibody

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  • Q: What band size(s) are detected when using AF-493-NA for Western blot?

    A: This antibody detects Recombinant Mouse VEGF164 at 42 kDa under non-reducing conditions and 21 kDa under reducing conditions.  Band sizes with natural samples may vary slightly depending on any post-translational modifications.  

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