Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Bioassay, ELISA Development, IHC frozen fixed

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VEGFR1/Flt-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VEGFR1/Flt-1
Ser27-Glu759
Accession # P35969

Specificity

Detects mouse VEGFR1/Flt-1 in ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse VEGFR1/Flt-1 Antibody

Cell Proliferation Induced by VEGF164and Neutralization by Mouse VEGFR1/Flt‑1 Antibody.

Cell Proliferation Induced by VEGF164and Neutralization by Mouse VEGFR1/Flt‑1 Antibody.

Recombinant Mouse VEGF164 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF164(5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND50 is typically 2-8 µg/mL.

Detection of Mouse VEGFR1/Flt-1 by Western Blot

Detection of Mouse VEGFR1/Flt-1 by Western Blot

Western blot analysis shows lower VEGFR1 and VEGFR2 expression in emphysematous tissues compared to controls. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19930612), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VEGFR1/Flt-1 by Western Blot

Detection of Mouse VEGFR1/Flt-1 by Western Blot

Western blot analysis shows lower VEGFR1 and VEGFR2 expression in emphysematous tissues compared to controls. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19930612), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1, CD31, F4/80, and CD11b expression in the pre-implantation mouse uterus. IHC and double staining IF were performed on E3.5 uterine cross-sections. (A) Schematic representation of an E3.5 mouse uterus showing lumen (arrowheads), glands, stroma (s), and myometrium (myo). (B) ECs, detected by CD31 staining (brown), are observed throughout the stroma and myometrium, similar to the non-pregnant state. (C) Macrophages, detected by F4/80 staining (brown), are observed throughout the stroma and are abundant adjacent to the lumen and glands at E3.5. (D) VEGFR-1+ cells (brown), are distributed throughout the stroma and cell associated VEGFR-1 expression highlighted in the inset. (E) Double staining for VEGFR-1 (red) and CD31 (green) demonstrates expression of VEGFR-1 on CD31+ ECs throughout the stroma. (F) VEGFR-1+ cells (red) and F4/80+ macrophages (green) are distributed throughout the stroma. VEGFR-1 and F4/80 co-expression is not observed. (G) VEGFR-1+ cells (red) and CD11b+ monocytes (green) are distributed throughout the stroma. VEGFR-1 and CD11b co-expression is not observed. L, lumen. Scale bars B, C = 100 μm. Scale bars D – F = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1 expression in endothelial cells and macrophages in the post-implantation uterus. H&E and double staining IF were performed on E6.5 frontal uterine sections. (A) H&E of a post-implantation mouse uterus showing the embryo (e), anti-mesometrial (am) and mesometrial (m) areas. (B-F) VEGFR-1+ cells (red) are observed in the decidua, with abundant expression in the primary decidual zone surrounding the implanted embryo. (B) Double-staining for VEGFR-1+ (red) and CD31+ (green) cells demonstrates expression of VEGFR-1 in a subset of CD31+ ECs. (C) Double-staining for VEGFR-1+ (red) and endomucin+ (green) cells demonstrates expression of VEGFR-1 in a subset of endomucin+ ECs. (D) Double-staining for VEGFR-1+ (red) and VE-cadherin+ (green) cells demonstrates expression of VEGFR-1 in VE-cadherin+ ECs. (E) Double-staining for VEGFR-1+ (red) and CD11b+ (green) cells demonstrates that VEGFR-1 is not expressed in CD11b+ monocytes. (F) Double-staining for VEGFR-1+ (red) and F4/80+ (green) cells demonstrates that VEGFR-1 is not expressed in F4/80+ macrophages. VEGFR-1+ cells are adjacent to CD11b+ monocytes and F4/80+ macrophages. White boxes in (B-F) indicate areas of the uteri magnified below (B1-F1). (A-F) Scale bar = 500 μm. (B1-F1) Scale bar = 20 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1, CD31, F4/80, and CD11b expression in the pre-implantation mouse uterus. IHC and double staining IF were performed on E3.5 uterine cross-sections. (A) Schematic representation of an E3.5 mouse uterus showing lumen (arrowheads), glands, stroma (s), and myometrium (myo). (B) ECs, detected by CD31 staining (brown), are observed throughout the stroma and myometrium, similar to the non-pregnant state. (C) Macrophages, detected by F4/80 staining (brown), are observed throughout the stroma and are abundant adjacent to the lumen and glands at E3.5. (D) VEGFR-1+ cells (brown), are distributed throughout the stroma and cell associated VEGFR-1 expression highlighted in the inset. (E) Double staining for VEGFR-1 (red) and CD31 (green) demonstrates expression of VEGFR-1 on CD31+ ECs throughout the stroma. (F) VEGFR-1+ cells (red) and F4/80+ macrophages (green) are distributed throughout the stroma. VEGFR-1 and F4/80 co-expression is not observed. (G) VEGFR-1+ cells (red) and CD11b+ monocytes (green) are distributed throughout the stroma. VEGFR-1 and CD11b co-expression is not observed. L, lumen. Scale bars B, C = 100 μm. Scale bars D – F = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1 expression in endothelial cells and macrophages in the post-implantation uterus. H&E and double staining IF were performed on E6.5 frontal uterine sections. (A) H&E of a post-implantation mouse uterus showing the embryo (e), anti-mesometrial (am) and mesometrial (m) areas. (B-F) VEGFR-1+ cells (red) are observed in the decidua, with abundant expression in the primary decidual zone surrounding the implanted embryo. (B) Double-staining for VEGFR-1+ (red) and CD31+ (green) cells demonstrates expression of VEGFR-1 in a subset of CD31+ ECs. (C) Double-staining for VEGFR-1+ (red) and endomucin+ (green) cells demonstrates expression of VEGFR-1 in a subset of endomucin+ ECs. (D) Double-staining for VEGFR-1+ (red) and VE-cadherin+ (green) cells demonstrates expression of VEGFR-1 in VE-cadherin+ ECs. (E) Double-staining for VEGFR-1+ (red) and CD11b+ (green) cells demonstrates that VEGFR-1 is not expressed in CD11b+ monocytes. (F) Double-staining for VEGFR-1+ (red) and F4/80+ (green) cells demonstrates that VEGFR-1 is not expressed in F4/80+ macrophages. VEGFR-1+ cells are adjacent to CD11b+ monocytes and F4/80+ macrophages. White boxes in (B-F) indicate areas of the uteri magnified below (B1-F1). (A-F) Scale bar = 500 μm. (B1-F1) Scale bar = 20 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1 expression in endothelial cells and macrophages in the post-implantation uterus. H&E and double staining IF were performed on E6.5 frontal uterine sections. (A) H&E of a post-implantation mouse uterus showing the embryo (e), anti-mesometrial (am) and mesometrial (m) areas. (B-F) VEGFR-1+ cells (red) are observed in the decidua, with abundant expression in the primary decidual zone surrounding the implanted embryo. (B) Double-staining for VEGFR-1+ (red) and CD31+ (green) cells demonstrates expression of VEGFR-1 in a subset of CD31+ ECs. (C) Double-staining for VEGFR-1+ (red) and endomucin+ (green) cells demonstrates expression of VEGFR-1 in a subset of endomucin+ ECs. (D) Double-staining for VEGFR-1+ (red) and VE-cadherin+ (green) cells demonstrates expression of VEGFR-1 in VE-cadherin+ ECs. (E) Double-staining for VEGFR-1+ (red) and CD11b+ (green) cells demonstrates that VEGFR-1 is not expressed in CD11b+ monocytes. (F) Double-staining for VEGFR-1+ (red) and F4/80+ (green) cells demonstrates that VEGFR-1 is not expressed in F4/80+ macrophages. VEGFR-1+ cells are adjacent to CD11b+ monocytes and F4/80+ macrophages. White boxes in (B-F) indicate areas of the uteri magnified below (B1-F1). (A-F) Scale bar = 500 μm. (B1-F1) Scale bar = 20 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1 expression in endothelial cells and macrophages in the post-implantation uterus. H&E and double staining IF were performed on E6.5 frontal uterine sections. (A) H&E of a post-implantation mouse uterus showing the embryo (e), anti-mesometrial (am) and mesometrial (m) areas. (B-F) VEGFR-1+ cells (red) are observed in the decidua, with abundant expression in the primary decidual zone surrounding the implanted embryo. (B) Double-staining for VEGFR-1+ (red) and CD31+ (green) cells demonstrates expression of VEGFR-1 in a subset of CD31+ ECs. (C) Double-staining for VEGFR-1+ (red) and endomucin+ (green) cells demonstrates expression of VEGFR-1 in a subset of endomucin+ ECs. (D) Double-staining for VEGFR-1+ (red) and VE-cadherin+ (green) cells demonstrates expression of VEGFR-1 in VE-cadherin+ ECs. (E) Double-staining for VEGFR-1+ (red) and CD11b+ (green) cells demonstrates that VEGFR-1 is not expressed in CD11b+ monocytes. (F) Double-staining for VEGFR-1+ (red) and F4/80+ (green) cells demonstrates that VEGFR-1 is not expressed in F4/80+ macrophages. VEGFR-1+ cells are adjacent to CD11b+ monocytes and F4/80+ macrophages. White boxes in (B-F) indicate areas of the uteri magnified below (B1-F1). (A-F) Scale bar = 500 μm. (B1-F1) Scale bar = 20 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1, CD31, F4/80, and CD11b expression in the pre-implantation mouse uterus. IHC and double staining IF were performed on E3.5 uterine cross-sections. (A) Schematic representation of an E3.5 mouse uterus showing lumen (arrowheads), glands, stroma (s), and myometrium (myo). (B) ECs, detected by CD31 staining (brown), are observed throughout the stroma and myometrium, similar to the non-pregnant state. (C) Macrophages, detected by F4/80 staining (brown), are observed throughout the stroma and are abundant adjacent to the lumen and glands at E3.5. (D) VEGFR-1+ cells (brown), are distributed throughout the stroma and cell associated VEGFR-1 expression highlighted in the inset. (E) Double staining for VEGFR-1 (red) and CD31 (green) demonstrates expression of VEGFR-1 on CD31+ ECs throughout the stroma. (F) VEGFR-1+ cells (red) and F4/80+ macrophages (green) are distributed throughout the stroma. VEGFR-1 and F4/80 co-expression is not observed. (G) VEGFR-1+ cells (red) and CD11b+ monocytes (green) are distributed throughout the stroma. VEGFR-1 and CD11b co-expression is not observed. L, lumen. Scale bars B, C = 100 μm. Scale bars D – F = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1, CD31, F4/80, and CD11b expression in the pre-implantation mouse uterus. IHC and double staining IF were performed on E3.5 uterine cross-sections. (A) Schematic representation of an E3.5 mouse uterus showing lumen (arrowheads), glands, stroma (s), and myometrium (myo). (B) ECs, detected by CD31 staining (brown), are observed throughout the stroma and myometrium, similar to the non-pregnant state. (C) Macrophages, detected by F4/80 staining (brown), are observed throughout the stroma and are abundant adjacent to the lumen and glands at E3.5. (D) VEGFR-1+ cells (brown), are distributed throughout the stroma and cell associated VEGFR-1 expression highlighted in the inset. (E) Double staining for VEGFR-1 (red) and CD31 (green) demonstrates expression of VEGFR-1 on CD31+ ECs throughout the stroma. (F) VEGFR-1+ cells (red) and F4/80+ macrophages (green) are distributed throughout the stroma. VEGFR-1 and F4/80 co-expression is not observed. (G) VEGFR-1+ cells (red) and CD11b+ monocytes (green) are distributed throughout the stroma. VEGFR-1 and CD11b co-expression is not observed. L, lumen. Scale bars B, C = 100 μm. Scale bars D – F = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

Detection of Mouse Mouse VEGFR1/Flt-1 Antibody by Immunohistochemistry

VEGFR-1 expression in endothelial cells and macrophages in the post-implantation uterus. H&E and double staining IF were performed on E6.5 frontal uterine sections. (A) H&E of a post-implantation mouse uterus showing the embryo (e), anti-mesometrial (am) and mesometrial (m) areas. (B-F) VEGFR-1+ cells (red) are observed in the decidua, with abundant expression in the primary decidual zone surrounding the implanted embryo. (B) Double-staining for VEGFR-1+ (red) and CD31+ (green) cells demonstrates expression of VEGFR-1 in a subset of CD31+ ECs. (C) Double-staining for VEGFR-1+ (red) and endomucin+ (green) cells demonstrates expression of VEGFR-1 in a subset of endomucin+ ECs. (D) Double-staining for VEGFR-1+ (red) and VE-cadherin+ (green) cells demonstrates expression of VEGFR-1 in VE-cadherin+ ECs. (E) Double-staining for VEGFR-1+ (red) and CD11b+ (green) cells demonstrates that VEGFR-1 is not expressed in CD11b+ monocytes. (F) Double-staining for VEGFR-1+ (red) and F4/80+ (green) cells demonstrates that VEGFR-1 is not expressed in F4/80+ macrophages. VEGFR-1+ cells are adjacent to CD11b+ monocytes and F4/80+ macrophages. White boxes in (B-F) indicate areas of the uteri magnified below (B1-F1). (A-F) Scale bar = 500 μm. (B1-F1) Scale bar = 20 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VEGFR1/Flt-1 by Western Blot

Detection of Mouse VEGFR1/Flt-1 by Western Blot

Tumor vascularization and metastasis are enhanced in beta IV‐ECKO mice. (A, B) Immunoblots show the level of endogenous beta IV‐spectrin along with the indicated receptor levels and activation of VEGF signaling effectors in freshly isolated primary ECs from control versus beta IV‐ECKO tumors. Graphs represent densitometry quantifications based on three biological repeats normalized to beta ‐actin loading control. p values are indicated in each graph. (C) Shown are representative IHC images of tumor sections stained with CD31 (indicated by black arrows). (D, E) Graphs show the number of vessels counted per mouse tumor section and the cross‐section area of individual blood vessels (n = 6 mice for control and beta IV‐ECKO). * p = 0.003 relative to control group. (F) Shown are representative images of lung and brain isolated from control and beta IV‐ECKO mice at necropsy. (G) Graph represents the average number of metastatic tumor nodules on the surface of different tissues per mouse (n = 7 mice for control and n = 8 for beta IV‐ECKO mice). Error bars represented by SEM. *p < 0.04 relative to control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37680049), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse VEGFR1/Flt-1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse VEGFR1/Flt‑1 Fc Chimera (Catalog # 471-F1)

Neutralization

Measured by its ability to neutralize VEGF164-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 5 ng/mL Recombinant Mouse VEGF164.

Mouse VEGFR1/Flt-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse VEGFR1/Flt-1 Biotinylated Antibody (Catalog # BAF471)
  • Standard: Recombinant Mouse VEGFR1/Flt-1 Fc Chimera Protein, CF (Catalog # 471-F1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGFR1/Flt-1

VEGFR1 is one of the five receptor tyrosine kinases (RTKs) (VEGFR1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGFR1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGFR1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGFR1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGFR1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

References

  1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
  2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Gene Symbol

FLT1

UniProt

P35969

Additional VEGFR1/Flt-1 Products

Product Documents for Mouse VEGFR1/Flt-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse VEGFR1/Flt-1 Antibody

For research use only

Citations for Mouse VEGFR1/Flt-1 Antibody

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