Mouse Wnt-5b Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Wnt-5b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-5a, -8a, -8b, -9b, -10a, -10b, or recombinant human Wnt-7b is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 325004
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Wnt‑5b
    Ser55-Ser95 and Lys246-Glu315 connected by a Gly-Ser linker
    Accession # NP_033551
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Wnt‑5b in NMuMG Mouse Cell Line. Wnt‑5b was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using Rat Anti-Mouse Wnt‑5b Monoclonal Antibody (Catalog # MAB3006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-5b

Wnt proteins are secreted glycoproteins with a conserved pattern of 23-24 cysteine residues that play critical roles in both carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1-3). Wnt-5b is a 49 kDa glycoprotein of the subgroup of Wnts that is implicated in the Wnt/Ca2+ pathway (3-6). It is not axis-inducing in Xenopus embryos and only weakly transforms C57MG mammary epithelial cells. The non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling (3). Mouse Wnt-5b is synthesized as a 359 amino acid (aa) precursor that contains a 17 aa signal sequence and a 342 aa mature region. It is ubiquitously expressed at low but increasing levels throughout embryonic development (4, 5, 7). In adult mice, Wnt-5b is expressed in heart, liver, brain, lung, testes, kidney, and pancreas (4, 8). Wnt-5b appears to promote adipogenesis. It is upregulated in early adipogenesis. Also, Wnt-5b overexpression in 3T3-L1 cells partially inhibits canonical Wnt suppression of adipogenesis (9, 10). Human Wnt-5b polymorphisms have been associated with Type II diabetes (9). Although Wnt-5a and Wnt-5b share 83% aa identity, they show differential expression and regulation of cyclin D1 and p130 during endochondral bone development. Together, they appear to coordinate chondrocyte proliferation and differentiation (11). Mature mouse Wnt-5b shows 94%, 98%, 90%, and 88% aa sequence identity with mature human, rat, chick, and Xenopus Wnt-5b, respectively.

  • References:
    1. Miller, J.R. (2002) Genome Biol. 3:3001.
    2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381. 
    3. Veeman, M.T. et al. (2003) Developmental Cell 5:367. 
    4. Gavin, B. J. et al. (1990) Genes Dev. 4:2319. 
    5. Nusse, R. and H.E. Varmus (1992) Cell 69:1073.
    6. Wong, G.T. et al. (1994) Mol. Cell. Biol. 14:6278.
    7. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
    8. Heller, R.S. et al. (2002) Dev. Dyn. 225:260.
    9. Kanazawa, A. et al. (2004) Am. J. Hum. Genet. 75:832.
    10. Kanazawa, A. et al. (2005) Biochem. Biophys. Res. Com. 330:505.
    11. Yang, Y. et al. (2003) Development 130:1003.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 5b
  • Entrez Gene IDs:
    81029 (Human)
  • Alternate Names:
    MGC2648; protein Wnt-5b; wingless-type MMTV integration site family, member 5B; WNT-5B protein; Wnt5b; Wnt-5b
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