Mouse XCL1/Lymphotactin Biotinylated Antibody

Catalog #: BAF486
1 Citation Datasheet / COA / SDS

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Mouse XCL1/Lymphotactin Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Lymphotactin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse XCL1/Lymphotactin

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)

Immunohistochemistry

5-15 µg/mL

See below

Mouse XCL1/Lymphotactin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse XCL1/Lymphotactin Antibody (Catalog # MAB486)

Standard: Recombinant Mouse XCL1/Lymphotactin Protein (Catalog # 486-LT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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XCL1/Lymphotactin in Mouse Thymus. XCL1/Lymphotactin was detected in perfusion fixed frozen sections of mouse thymus using Mouse XCL1/Lymphotactin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF486) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCL1/Lymphotactin

Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.

References

Kelner, G. et al. (1994) Science 266:1395.
Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Long Name

Chemokine [C Motif] Ligand 1

Entrez Gene IDs

6375 (Human); 16963 (Mouse)

Alternate Names

ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1

Product Datasheets

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Related Research Areas

Citation for Mouse XCL1/Lymphotactin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A parenteral DNA vaccine protects against pneumonic plague.
Authors: Yamanaka H, Hoyt T, Yang X, Bowen R, Golden S, Crist K, Becker T, Maddaloni M, Pascual DW
Vaccine, 2010;28(18):3219-30.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development