Mouse XCL1/Lymphotactin Antibody

Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Mouse XCL1/Lymphotactin Antibody.

Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse XCL1/Lymphotactin (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486). The ND₅₀ is typically 5-15 µg/mL.

Mouse XCL1 / Lymphotactin ELISA Standard Curve

Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) was serially diluted and captured by Rat Anti-Mouse XCL1/Lymphotactin Monoclonal Antibody (Catalog # MAB486) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse XCL1/Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Detection of XCL1/Lymphotactin by Immunohistochemistry

Immunofluorescence analysis of chemokine-C-motif ligand 1 (XCL1) localization in the lumbar (L4 to L6) spinal cord 7 days after chronic constriction injury (CCI) of the sciatic nerve in mice. Dorsal (A–D, I–L, R–U) and ventral (E–H, M–P, W–Z) parts of lumbar spinal cord (a,b,c) were shown as an approximate fragments of selected images. Representative immunofluorescence images from colocalization analysis performed on spinal cord, paraffin-embedded 7 μM microtome slices: XCL1 (green: A, E, I, M, R, W) with astroglia marker glial fibrillary acidic protein; (GFAP, red: B, F); microglia marker ionized calcium-binding adaptor molecule 1; (IBA1, red: J, N); and with neuronal marker neuronal nucleus; (NeuN, red: S, X), Nuclei are in blue (C, G, K, O, T, Y). High magnification, three-dimensional image rendering shows XCL1 localization inside GFAP-positive cells (yellow: D, H). Scale bars: 50 μm (a, b, c), 500 μm (A–Z). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36618360), licensed under a CC-BY license. Not internally tested by R&D Systems.