Detects XCL1/Lymphotactin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, and recombinant mouse MCP-5 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse XCL1/Lymphotactin
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Perfusion fixed frozen sections of mouse small intestine (Peyer's patch) and thymus
Measured by its ability to neutralize XCL1/Lymphotactin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human XCR1. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 0.15 µg/mL Recombinant Mouse XCL1/Lymphotactin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Mouse XCL1/Lymphotactin Antibody. Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human XCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse XCL1/Lymphotactin (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486). The ND50 is typically 5-15 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.
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