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Mouse XCL1/Lymphotactin Antibody

Catalog #: AF486
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF486
AF486-SP

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Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Mouse XCL1/Lymphotactin Antibody.
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Citations (6)
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Mouse XCL1/Lymphotactin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects XCL1/Lymphotactin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, and recombinant mouse MCP-5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse XCL1/Lymphotactin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse small intestine (Peyer's patch) and thymus
Neutralization
Measured by its ability to neutralize XCL1/Lymphotactin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human XCR1. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 0.15 µg/mL Recombinant Mouse XCL1/Lymphotactin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Mouse XCL1/Lymphotactin Antibody. View Larger

Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Mouse XCL1/Lymphotactin Antibody. Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse XCL1/Lymphotactin (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486). The ND50 is typically 5-15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCL1/Lymphotactin

Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.

References
  1. Kelner, G. et al. (1994) Science 266:1395.
  2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
Long Name
Chemokine [C Motif] Ligand 1
Entrez Gene IDs
6375 (Human); 16963 (Mouse)
Alternate Names
ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1

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Citations for Mouse XCL1/Lymphotactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Microbiota configuration determines nutritional immune optimization
    Authors: Han, SJ;Stacy, A;Corral, D;Link, VM;De Siqueira, MK;Chi, L;Teijeiro, A;Yong, DS;Perez-Chaparro, PJ;Bouladoux, N;Lim, AI;Enamorado, M;Belkaid, Y;Collins, N;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, Immunohistochemistry
  2. New insights into the analgesic properties of the XCL1/XCR1 and XCL1/ITGA9 axes modulation under neuropathic pain conditions - evidence from animal studies
    Authors: Ciechanowska A, Rojewska E, Piotrowska A et al.
    Frontiers in immunology
  3. Cytotoxic T cells swarm by homotypic chemokine signalling
    Authors: Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al.
    eLife
  4. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018-02-08;172(5):1022-1037.e14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. A parenteral DNA vaccine protects against pneumonic plague.
    Authors: Yamanaka H, Hoyt T, Yang X, Bowen R, Golden S, Crist K, Becker T, Maddaloni M, Pascual DW
    Vaccine, 2010-03-01;28(18):3219-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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