NorthernLights™ Fluorescent Secondary Antibodies

With more than 6000 primary antibodies available, the addition of the NorthernLights line of fluorescent secondary antibodies makes R&D Systems your total source for immunofluorescence reagents.

NorthernLights Antibody/Label Catalog # Abs/Em Maxima Laser (Ex) Comparable Fluorochromes
NL-493 anti-Rabbit IgG
NL-493 anti-Mouse IgG
NL-493 anti-Goat IgG
NL-493 Streptavidin
493/514 Argon (488) FITC (492/520)
Cy™2 (489/506)
Alexa Fluor® 488 (494/519)
NL-557 anti-Rabbit IgG
NL-557 anti-Mouse IgG
NL-557 anti-Goat IgG
NL-557 Streptavidin
557/575 Krypton (568)
HeNe (543)
Phycoerythrin (565/575)
Rhodamine Red X (570/590)
Cy™3 (548/562)
NL-637 anti-Rabbit IgG
NL-637 anti-Mouse IgG
NL-637 anti-Goat IgG
NL-637 Streptavidin
637/658 HeNe (633) Allophycocyanin (645/660)
Alexa Fluor® 647 (650/668)
Cy™5 (650/670)

NorthernLights are bright and fade-resistant antibodies recognizing mouse, goat, and rabbit IgG. Like all R&D Systems antibodies, NorthernLights antibody conjugates are highly specific and deliver a high signal-to-noise ratio. Also available are streptavidin conjugates for labeling biotinylated cells and tissues. Their spectral properties are similar to several widely used fluorochromes, and are therefore suitable for use with common filter sets and/or lasers (Table). These secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy (Figure 1). NorthernLights secondary antibodies are stable when exposed to alcohols and xylene, as well as DPX mounting medium. They have been tested for their suitability in both immunochemistry and flow cytometry (Figure 2 and 3).

Fluorescent Secondary Antibodies 1 Fluorescent Secondary Antibodies 2
Figure 1. Rat cortical stem cell differentiation was monitored using multi-color immunocytochemistry. Neural progenitors were labeled with a Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF2736) and stained with a NorthernLights-493-conjugated Donkey Anti-Goat Secondary Antibody (R&D Systems, Catalog # NL003; green). Differentiated neurons were labeled with a Mouse Neuron-specific Anti-beta-III Tubulin Monoclonal Antibody (TuJ1; R&D Systems, Catalog # MAB1195) and stained using  NorthernLights-557-conjugated Donkey Anti-Mouse Secondary Antibody (R&D Systems, Catalog # NL007; red). Nuclei were stained with DAPI (blue). Figure 2. Glucagon is selectively expressed by some cells of the mouse pancreatic beta cell line beta-TC6. Glucagon was detected using an Anti-Human/Mouse Glucagon Monoclonal Antibody (R&D Systems, Catalog # MAB1249) and cells were stained with a NorthernLights-557-conjugated Donkey Anti-Mouse Secondary Antibody (R&D Systems, Catalog # NL007; red). The nuclei were counterstained with DAPI (blue).
Fluorescent Secondary Antibodies 3  
Figure 3. Jurkat T cells were fixed, permeabilized, and incubated with a Mouse Anti-Human Akt1 Monoclonal Antibody (R&D Systems, Catalog # MAB17751). Cells were then stained with a NorthernLights-637-conjugated Donkey Anti-Mouse IgG Secondary Antibody (R&D Systems, Catalog # NL008; red histogram). Control stain­ing was done by incubating cells with a Mouse IgG1 Isotype Control Monoclonal Antibody (R&D Systems, Catalog # MAB002), followed by the staining with NorthernLights-637-conjugated Donkey Anti-Mouse IgG Secondary Antibody (R&D Systems, Catalog # NL008; black histogram).