Notch-1 Antibody (OTI3E12)

Novus Biologicals | Catalog # NBP1-48289

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3E12
View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens Notch homolog 1, translocation-associated (Drosophila) (NOTCH1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

270.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Notch-1 Antibody (OTI3E12)

Western Blot: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Western Blot: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Western Blot: Notch-1 Antibody (3E12) [NBP1-48289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HepG2 cells using anti Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (OTI3E12) [NBP1-48289]

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.

Applications for Notch-1 Antibody (OTI3E12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Reviewed Applications

Read 2 reviews rated 5 using NBP1-48289 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Notch-1

Notch1 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development

Alternate Names

Notch1, TAN1

Entrez Gene IDs

4851 (Human)

Gene Symbol

NOTCH1

UniProt

P46531

Additional Notch-1 Products

Product Documents for Notch-1 Antibody (OTI3E12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Notch-1 Antibody (OTI3E12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Notch-1 Antibody (OTI3E12)

Customer Reviews for Notch-1 Antibody (OTI3E12) (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Notch-1 Antibody (OTI3E12)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Name: Alissa Taylor
    Application: Western Blot
    Sample Tested: full length human Notch1 plasmid transfected Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 10/28/2014
  • Name: Alissa Taylor
    Application: Immunohistochemistry-Paraffin
    Verified Customer | Posted 10/28/2014

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Notch-1 Antibody (OTI3E12)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

    A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies