Notch-1 Antibody (OTI3E12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73042

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Reverse Phase Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3E12

Format

Azide and BSA Free
View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

270.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Notch-1 Antibody (OTI3E12) - Azide and BSA Free

Western Blot: Notch-1 Antibody (OTI3E12)Azide and BSA Free [NBP2-73042]

Western Blot: Notch-1 Antibody (OTI3E12)Azide and BSA Free [NBP2-73042]

Western Blot: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of HepG2 cells using anti Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042]

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.

Applications for Notch-1 Antibody (OTI3E12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50RPPA 1:75

Reverse Phase Protein Array

1:75

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

Additional Notch-1 Products

Product Documents for Notch-1 Antibody (OTI3E12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Notch-1 Antibody (OTI3E12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Notch-1 Antibody (OTI3E12) - Azide and BSA Free

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  • Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

    A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

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