Notch-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-95105

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2455-2555 of human Notch-1 (NP_060087.3). VPEHPFLTPSPESPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

272 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Notch-1 Antibody - BSA Free

Western Blot: Notch-1 AntibodyAzide and BSA Free [NBP2-95105]

Western Blot: Notch-1 AntibodyAzide and BSA Free [NBP2-95105]

Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1.Exposure time: 300s.
Notch-1 Antibody - Azide and BSA Free

Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Notch-1 Antibody - Azide and BSA Free

Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Notch-1 Antibody - Azide and BSA Free

Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Notch-1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of U2OS cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Notch-1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of HeLa cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Notch-1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of A-549 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Notch-1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of PC-12 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Notch-1 Antibody - Azide and BSA Free

Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Notch-1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] -

Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of NIH/3T3 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Notch-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

Additional Notch-1 Products

Product Documents for Notch-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Notch-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Notch-1 Antibody - BSA Free

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  • Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

    A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

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