PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free

Name: PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-22212
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-22212

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 6G10

Format

BSA Free

View all PI 3-Kinase p85 alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PI 3-Kinase p85 alpha (AA: 159-388) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

83.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free

Western Blot: PI 3-Kinase p85 alpha Antibody (6G10)BSA Free [NBP2-22212]

Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)

Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free [NBP2-22212]

Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HepG2 cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free [NBP2-22212]

Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of rectum cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.

Flow Cytometry: PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free [NBP2-22212]

Flow Cytometry: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of NIH3T3 cells using PI 3 Kinase p85 alpha mouse mAb (green) and negative control (red).

Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free [NBP2-22212]

Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HeLa cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free [NBP2-22212]

Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of cervical cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.

ELISA: PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free [NBP2-22212]

ELISA: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

View 4 more images

Applications for PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Application Notes

This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PI 3-Kinase p85 alpha

Phosphatidylinositol 3-kinase (PI3K) p85 alpha is a regulatory subunit that heterodimerizes with a catalytic subunit to form a Class IA PI3K enzyme complex, which plays an important role in the immune system (1,2). The P13K pathway is involved in many diverse processes including growth, metabolism, proliferation, and survival (2). PI3Ks are typically activated by cytokine receptors and are responsible for phosphorylation of the 3'-hydroxyl group of PI and its derivatives (3). p85 alpha is one of five regulatory subunit proteins which also includes p55 alpha, p50 alpha, p85 beta, and p55 gamma, and can bind to two of the three catalytic subunits (p110 alpha or p110 delta) (1,2). p85 alpha, p55 alpha, and p50 alpha proteins are all synthesized by the same PIK3R1 gene by alternative splicing (1). Structurally, PI 3-Kinase p85 alpha contains SRC homology 3 (SH3 domain), followed by a Bcr homology (BH) domain flanked by two proline-rich regions, then a N-terminal SH2 domain, an inter-SH2 domain, and C-terminal SH2 domain (1,2). PI 3-Kinase p85 alpha protein consists of 724 amino acids (aa) in length with a theoretical molecular weight of 83.5 kDa (2,4). The primary role for the p85 subunit is interaction with cell surface receptors and acts as an adapter for the stabilization and recruitment of the p110 catalytic subunit to the plasma membrane (1,3). Additionally, p85 has been shown to function in both interleukin-2 receptor (IL2R) and erythropoietin receptor (EpoR) endocytosis (3).

The PI3K pathway functions in a broad range of cellular processes, so it is understandable that pathway dysfunction can lead to an array of diseases and disorders (2,5). Elevated PI3K signaling is a key feature of many cancers (5). PI3K pathway dysregulation has also been implicated in neurological, metabolic, and cardiovascular disorders (5). Furthermore, both overactivation or under-activation of the PI3K delta (p85 alpha subunit + p110 delta subunit) pathway has been shown to cause immunodeficiency and pathologies related to immune system dysfunction (2). Therapeutics to target the PI3K pathway and treat related cancers include PI3K inhibitors and, specifically, isoform-selective inhibitors which have a lot of promise when used as part of a combination therapy (5).

References

1. Okkenhaug, K., & Vanhaesebroeck, B. (2001). New responsibilities for the PI3K regulatory subunit p85 alpha. Science's STKE : signal transduction knowledge environment. https://doi.org/10.1126/stke.2001.65.pe1

2. Nunes-Santos, C. J., Uzel, G., & Rosenzweig, S. D. (2019). PI3K pathway defects leading to immunodeficiency and immune dysregulation. The Journal of allergy and clinical immunology. https://doi.org/10.1016/j.jaci.2019.03.017

3. Chen, P. H., Yao, H., & Huang, L. J. (2017). Cytokine Receptor Endocytosis: New Kinase Activity-Dependent and -Independent Roles of PI3K. Frontiers in endocrinology. https://doi.org/10.3389/fendo.2017.00078

4. Uniprot (P27986)

5. Fruman, D. A., Chiu, H., Hopkins, B. D., Bagrodia, S., Cantley, L. C., & Abraham, R. T. (2017). The PI3K Pathway in Human Disease. Cell. https://doi.org/10.1016/j.cell.2017.07.029

Long Name

PI 3-Kinase p85 alpha

Alternate Names

GRB1, PI 3Kinase p85 alpha, PIK3R1

Gene Symbol

PIK3R1

Additional PI 3-Kinase p85 alpha Products

Product Documents for PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free

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Product Specific Notices for PI 3-Kinase p85 alpha Antibody (6G10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.