Proteome Profiler Human Phospho-Immunoreceptor Array Kit

Contains 4 membranes - each spotted in duplicate with 59 different immunoreceptor antibodies
Catalog # Availability Size / Price Qty
ARY004B
Product Details
Citations (2)
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Proteome Profiler Human Phospho-Immunoreceptor Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative phosphorylation of human immunoreceptors. Validated for analyte detection in cell lysates.

Troubleshooting Guide

Key Benefits

  • Detects phosphorylation of 59 human ITAM/ITIM-associated immunoreceptors simultaneously
  • Requires no specialized equipment
 

 

Principle of the Assay

The Proteome Profiler Human Phospho-Immunoreceptor Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Tyrosine phosphorylation of the captured proteins is detected with an HRP-conjugated pan phospho-tyrosine antibody (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount phosphorylation in the bound analyte.

Why Use an Antibody Array to Detect Receptor Phosphorylation?

Determining the phosphorylation of multiple immunoreceptors in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple phosphorylations in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents

This kit contains the following azide-free, fluorochrome-conjugated antibodies to verify human stem cell pluripotency:

  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Lysis Buffer
  • Wash Buffer
  • HRP-conjugated Anti-phospho-tyrosine Detection Antibody
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

 
Simultaneously detect the relative phosphorylation levels of these immunoreceptors in a single sample.
2B4/SLAMF4 FcRH1/IRTA5 NKp46/NCR1
BLAME/SLAMF8 FcRH2/IRTA4 NKp80/KLRF1
BTLA FcRH4/IRTA1 NTB-A/SLAMF6
CD3 epsilon FcRH5/IRTA2 PD-1
CD5 ILT2/CD85j PECAM/CD31)
CD6 ILT3/CD85k SHIP-1
CD28 ILT4/CD85d SHP-1
CD84/SLAMF5 ILT5/CD85a SHP-2
CD229/SLAMF3 ILT6/CD85e Siglec-2/CD22
CEACAM-1 Integrin beta 3/CD61 Siglec-3/CD33
CLEC-1 KIR2DL4 Siglec-5
CLEC-2 LAIR-1 Siglec-7
CRACC/SLAMF7 LAIR-2 Siglec-9
CTLA-4/CD152 LMIR1/CD300A Siglec-10
DCIR/CLEC4A LMIR2/CD300C SIRP-beta 1
Dectin-1/CLEC7A LMIR3/CD300LF SLAM/CD150
DNAM-1 LMIR6/CD300LE TREM-1
Fc epsilon RII/CD23 MDL-1/CLEC5A TREM-2
Fc gamma RIIA NKp30/NCR3 TREML1/TLT-1
Fc gamma RIIIA/B NKp44/NCR2  

Data Examples
Figure 1A.
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Figure 1A: The Human Phospho-Immunoreceptor Array detects multiple tyrosine phosphorylated immunoreceptors in lysates prepared from cells activated by antibodymediated cross-linking of cell-surface immunoreceptors. THP-1 human acute monocytic leukemia cells were incubated with goat anti-Fcγ RIIA antibody (R&D Systems, Catalog # AF1875) or normal goat IgG (R&D Systems, Catalog # AB-108-C) followed by incubation with a donkey anti-goat IgG antibody (R&D Systems, Catalog # AF109) for 5 minutes [Maresco, D.L. et al. (1999) J. Immunol. 162:6458].

Figure 1B.
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Figure 1B: Jurkat human acute T cell leukemia cells were incubated with a mouse monoclonal anti-CD3ε antibody (R&D Systems, Catalog # MAB100) or mouse IgG1 isotype control (R&D Systems, Catalog # MAB002) followed by incubation with a goat anti-mouse antibody (R&D Systems, Catalog # AF007) for 5 minutes. 100 µg of lysate was run on each array.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Immunoreceptor Array Kit

R&D Systems Human Phospho-Immunoreceptor Array (Catalog # ARY004) is a rapid, sensitive, and economical tool used to simultaneously detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors, avoiding numerous immunoprecipitations and/or Western blots.

Alternate Names
Immunoreceptor Array Kit

Citations for Proteome Profiler Human Phospho-Immunoreceptor Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Fibroblast Growth Factor Receptor 3 (FGFR3) Associated with the CD20 Antigen Regulates the Rituximab-induced Proliferation Inhibition in B-cell Lymphoma Cells.
    Authors: Kotani N, Ishiura Y, Yamashita R, Ohnishi T, Honke K
    J Biol Chem, 2012;287(44):37109-18.  2012
  2. Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
    Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
    Haematologica, 2011;0(0):.  2011

FAQs

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