Rabbit IgG HRP-conjugated Antibody

(23 citations)
(1 Review)
  
  • Species Reactivity
    Rabbit
  • Specificity
    Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Rabbit IgG
  • Formulation
    Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
  • Label
    HRP (Horseradish Peroxidase)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Use at a 1:1000 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of RabbitIgG Primary Antibody by Western Blot Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60Polyclonal Antibody
(Catalog # AF1800) using a 1:1000 dilution ofHRP-conjugated Anti-Rabbit IgG Secondary Antibody
(Catalog # HAF008).  This experiment was conducted under reducingconditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells
    Authors: CT Yang, R Ma, RA Axton, M Jackson, AH Taylor, A Fidanza, L Marenah, J Frayne, JC Mountford, LM Forrester
    Stem Cells, 2017;35(4):886-897.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Antioxidant Treatment Induces Hyperactivation of the HPA Axis by Upregulating ACTH Receptor in the Adrenal and Downregulating Glucocorticoid Receptors in the Pituitary
    Authors: JP Prevatto, RC Torres, BL Diaz, PMRE Silva, MA Martins, VF Carvalho
    Oxid Med Cell Longev, 2017;2017(0):4156361.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  3. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
    Authors: KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: WB
  4. Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract
    Authors: P Bokiniec, S Shahbazian, SJ McDougall, BA Berning, D Cheng, IJ Llewellyn-, PG Burke, S McMullan, M Mühlenhoff, H Hildebrand, F Braet, M Connor, NH Packer, AK Goodchild
    J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: Functional Assay
  5. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target
    Sci Rep, 2016;6(0):37360.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. The GTPase ARF6 Controls ROS Production to Mediate Angiotensin II-Induced Vascular Smooth Muscle Cell Proliferation.
    Authors: Bourmoum M, Charles R, Claing A
    PLoS ONE, 2016;11(1):e0148097.
  7. Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
    Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
    PLoS ONE, 2015;10(4):e0121103.
    Species: Human
    Sample Type: Cell Lysates
    Application: Bioassay
  8. MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death.
    Authors: Chaudhuri A, Kabaria S, Choi D, Mouradian M, Junn E
    J Biol Chem, 2015;290(19):12425-34.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and gammadeltaT Cells.
    Authors: Shimizu K, Shinga J, Yamasaki S, Kawamura M, Dorrie J, Schaft N, Sato Y, Iyoda T, Fujii S
    PLoS ONE, 2015;10(6):e0131477.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Evolutionary Consequence of a Trade-Off between Growth and Maintenance along with Ribosomal Damages.
    Authors: Ying B, Honda T, Tsuru S, Seno S, Matsuda H, Kazuta Y, Yomo T
    PLoS ONE, 2015;10(8):e0135639.
    Species: Bacteria - E. Coli
    Sample Type: Cell Lysates
    Application: WB
  11. Symmorphosis through dietary regulation: a combinatorial role for proteolysis, autophagy and protein synthesis in normalising muscle metabolism and function of hypertrophic mice after acute starvation.
    Authors: Collins-Hooper H, Sartori R, Giallourou N, Matsakas A, Mitchell R, Makarenkova H, Flasskamp H, Macharia R, Ray S, Swann J, Sandri M, Patel K
    PLoS ONE, 2015;10(3):e0120524.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  12. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
    Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
    J Clin Invest, 2015;125(3):1243-54.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Type: Cell Lysates
    Application: WB
  13. Inflammation enhances IL-2 driven differentiation of cytolytic CD4 T cells.
    Authors: Workman A, Jacobs A, Vogel A, Condon S, Brown D
    PLoS ONE, 2014;9(2):e89010.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  14. MicroRNA-7 protects against 1-methyl-4-phenylpyridinium-induced cell death by targeting RelA.
    Authors: Choi D, Chae Y, Kabaria S, Chaudhuri A, Jain M, Li H, Mouradian M, Junn E
    J Neurosci, 2014;34(38):12725-37.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Propionibacterium acnes Induces IL-1beta secretion via the NLRP3 inflammasome in human monocytes.
    Authors: Qin M, Pirouz A, Kim M, Krutzik S, Garban H, Kim J
    J Invest Dermatol, 2014;134(2):381-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  16. Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.
    Authors: Wang J, Jenkins S, Lamartiniere C
    BMC Cancer, 2014;14(0):379.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  17. Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin.
    Authors: Sangokoya, Carolyn, Doss, Jennifer, Chi, Jen-Tsan
    PLoS Genet, 2013;9(4):e1003408.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  18. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
    Species: Human
    Sample Type: Cell Lysates
    Application: ELISA Development
  19. A fragment of the LG3 peptide of endorepellin is present in the urine of physically active mining workers: a potential marker of physical activity.
    Authors: Parker TJ, Sampson DL, Broszczak D
    PLoS ONE, 2012;7(3):e33714.
    Species: Human
    Sample Type: Urine
    Application: WB
  20. BMP-3 promotes mesenchymal stem cell proliferation through the TGF-beta/activin signaling pathway.
    Authors: Stewart A, Guan H, Yang K
    J. Cell. Physiol., 2010;223(3):658-66.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  21. The major outer membrane protein of a periodontopathogen induces IFN-beta and IFN-stimulated genes in monocytes via lipid raft and TANK-binding kinase 1/IFN regulatory factor-3.
    Authors: Lee SH, Kim JS, Jun HK
    J. Immunol., 2009;182(9):5823-35.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  22. Long-term high glucose concentration influences Akt, ERK1/2, and PTP1B protein expression in human aortic smooth muscle cells.
    Authors: Popov D, Nemecz M, Dumitrescu M, Georgescu A, Bohmer FD
    Biochem. Biophys. Res. Commun., 2009;388(1):51-5.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  23. Hyperoxia modulates TGF-beta/BMP signaling in a mouse model of bronchopulmonary dysplasia.
    Authors: Alejandre-Alcazar MA, Kwapiszewska G, Reiss I, Amarie OV, Marsh LM, Sevilla-Perez J, Wygrecka M, Eul B, Kobrich S, Hesse M, Schermuly RT, Seeger W, Eickelberg O, Morty RE
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(2):L537-49.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Expand to show all 23 Citations
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

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 ELISA Human Anonymous 10/31/2017
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Summary

ApplicationELISA
Sample TestedHuman recombinant antibody
SpeciesHuman

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