Key Product Details

Validated by

Biological Validation

Species Reactivity

Rabbit

Applications

Immunohistochemistry, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rabbit IgG

Specificity

Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Detection of Rabbit IgG Primary Antibody by Western Blot

Detection of Rabbit IgG Primary Antibody by Western Blot

Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of FoxP3 in Human Tonsil

Detection of FoxP3 in human tonsil using 5 μg/mL of Rabbit monoclonal anti-human FoxP3 primary antibody (MAB8214) followed by 30 min incubation with Goat anti Rabbit HRP conjugated secondary antibodies (Catalog # HAF008) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.
Detection of Human IgG by Western Blot

Detection of Human IgG by Western Blot

PARP-cleavage upon treatment with embryonic stimuli.Representative Western Blot analysis of PARP cleavage (full length PARP 116kDa; cleaved PARP 89 kDa) in non-differentiated (St-T1, lanes 1+2; KdS1, lanes 3+4) and decidualized (dSt-T1, lanes 5+6; dKdS1, lanes 7+8) ESCs after treatment with IITT+F vs. untreated controls, n = 3; beta -Actin (42kDa) served as a loading control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0121103), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Use at a 1:20 dilution 

Western Blot

Use at a 1:1000 dilution.

Reviewed Applications

Read 8 reviews rated 5 using HAF008 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

For research use only

Citations for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Customer Reviews for Rabbit IgG Horseradish Peroxidase-conjugated Antibody (8)

5 out of 5
8 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • Rabbit ab HRP conjugated
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 02/03/2026
    Rabbit HRP conjugated in HCT116
    1:1000 dilution, band is strong and appear quickly. Higher dilution would be beneficial. Used to detect GAPDH (NB100-56875)
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Secondary abs of Rabbit HRP conjugated
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 02/03/2026
    GAPDH (2275-PC-100) detected with Rabbit-HRP
    Clear signal with 1:1000 dilution, lower concentration can be used.
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Rabbit IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lines
    Species: Human
    Verified Customer | Posted 08/05/2025
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Rabbit IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line and NIH-3T3 mouse embryonic fibroblast cell line
    Species: Human
    Verified Customer | Posted 04/12/2021
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Rabbit IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver cells
    Species: Mouse
    Verified Customer | Posted 10/27/2019
    1:1000 Dilution in 5% nonfat dry milk in TBST for 1 hr at RT.
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Rabbit IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Muscle tissue, Liver tissue, Skin tissue and human liver cells, Sample Amount: 15 ug
    Species: Pig and Human
    Verified Customer | Posted 08/14/2019
    Dot blot test: A) Human liver neat, B) Human liver 1:2 dilution, C) Human liver 1:5 dilution, D) Pig muscle 1:10 dilution, E) Pig liver 1:10 dilution, and F) Pig skin 1:10 dilution.
    Rabbit IgG Horseradish Peroxidase-conjugated Antibody HAF008
  • Rabbit IgG HRP-conjugated Antibody
    Name: Danlee Enzler
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 04/24/2018
  • Rabbit IgG HRP-conjugated Antibody
    Name: Adrie Jones
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Human
    Verified Customer | Posted 10/31/2017

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Protocols

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FAQs for Rabbit IgG Horseradish Peroxidase-conjugated Antibody

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  • Q: May I know the buffer used in this product?

    A: It is supplied as a 0.2um filtered solution in sodium phosphate and Proclin 300

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