Rabbit IgG HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
HAF008
Detection of RabbitIgG Primary Antibody by Western Blot
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Product Details
Citations (35)
FAQs
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Reviews (2)

Rabbit IgG HRP-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Label
HRP (Horseradish Peroxidase)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of RabbitIgG Primary Antibody by Western Blot View Larger

Detection of RabbitIgG Primary Antibody by Western Blot Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60Polyclonal Antibody
(Catalog # AF1800) using a 1:1000 dilution ofHRP-conjugated Anti-Rabbit IgG Secondary Antibody
(Catalog # HAF008).  This experiment was conducted under reducingconditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Rabbit IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells After Long-Term Exposure to Simulated Microgravity
    Authors: A Dittrich, D Grimm, J Sahana, J Bauer, M Krüger, M Infanger, NE Magnusson
    Cell. Physiol. Biochem., 2018;45(2):429-445.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Low-dose paclitaxel downregulates MYC proto-oncogene bHLH transcription factor expression in colorectal carcinoma cells
    Authors: W Li, W Zhu, C Lv, H Qu, K Xu, H Li, H Li, Y Du, G Liu, Y Wang, HJ Wei, HY Zhao
    Oncol Lett, 2018;15(2):1881-1887.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
    Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
    PLoS ONE, 2018;13(5):e0195427.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  4. Antioxidant nitroxides protect hepatic cells from oxidative stress-induced cell death
    Authors: S Shinto, Y Matsuoka, M Yamato, KI Yamada
    J Clin Biochem Nutr, 2018;62(2):132-138.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  5. Development of matrix metalloproteinase-targeted probes for lung inflammation detection with positron emission tomography
    Authors: N Kondo, T Temma, K Aita, S Shimochi, K Koshino, M Senda, H Iida
    Sci Rep, 2018;8(1):1347.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  6. The Role of Diacylglycerol Acyltransferase (DGAT) 1 and 2 in Cardiac Metabolism and Function
    Authors: ND Roe, MK Handzlik, T Li, R Tian
    Sci Rep, 2018;8(1):4983.
    Applications: WB
  7. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
    Authors: KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: WB
  8. Variation in the early host-pathogen interaction of bovine macrophages with divergent UK Mycobacterium bovis strains
    Authors: K Jensen, IJ Gallagher, N Johnston, M Welsh, R Skuce, JL Williams, EJ Glass
    Infect. Immun., 2017;0(0):.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: WB
  9. Polymorphism of Receptor-Type Tyrosine-Protein Phosphatase Delta gene in the development of non-alcoholic fatty liver disease
    Authors: S Nakajima, H Tanaka, K Sawada, H Hayashi, T Hasebe, M Abe, C Hasebe, M Fujiya, T Okumura
    J. Gastroenterol. Hepatol., 2017;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: IHC
  10. Molluscum Contagiosum Virus MC159 abrogates cIAP1-NEMO interactions and inhibits NEMO polyubiquitination
    Authors: S Biswas, JL Shisler
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: WB
  11. Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells
    Authors: CT Yang, R Ma, RA Axton, M Jackson, AH Taylor, A Fidanza, L Marenah, J Frayne, JC Mountford, LM Forrester
    Stem Cells, 2017;35(4):886-897.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  12. Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract
    Authors: P Bokiniec, S Shahbazian, SJ McDougall, BA Berning, D Cheng, IJ Llewellyn-, PG Burke, S McMullan, M Mühlenhoff, H Hildebrand, F Braet, M Connor, NH Packer, AK Goodchild
    J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Functional Assay
  13. Antioxidant Treatment Induces Hyperactivation of the HPA Axis by Upregulating ACTH Receptor in the Adrenal and Downregulating Glucocorticoid Receptors in the Pituitary
    Authors: JP Prevatto, RC Torres, BL Diaz, PMRE Silva, MA Martins, VF Carvalho
    Oxid Med Cell Longev, 2017;2017(0):4156361.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  14. Antiangiogenic effects of catalpol on rat corneal�neovascularization
    Authors: Y Han, M Shen, LY Tang, G Tan, QC Yang, L Ye, LH Ye, N Jiang, GP Gao, Y Shao
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: WB
  15. Impaired regeneration in calpain-3 null muscle is associated with perturbations in mTORC1 signaling and defective mitochondrial biogenesis
    Authors: ME Yalvac, J Amornvit, C Braganza, L Chen, SA Hussain, KM Shontz, CL Montgomery, KM Flanigan, S Lewis, Z Sahenk
    Skelet Muscle, 2017;7(1):27.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target
    Sci Rep, 2016;6(0):37360.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  17. The GTPase ARF6 Controls ROS Production to Mediate Angiotensin II-Induced Vascular Smooth Muscle Cell Proliferation.
    Authors: Bourmoum M, Charles R, Claing A
    PLoS ONE, 2016;11(1):e0148097.
  18. Evolutionary Consequence of a Trade-Off between Growth and Maintenance along with Ribosomal Damages.
    Authors: Ying B, Honda T, Tsuru S, Seno S, Matsuda H, Kazuta Y, Yomo T
    PLoS ONE, 2015;10(8):e0135639.
    Species: Bacteria - E. Coli
    Sample Types: Cell Lysates
    Applications: WB
  19. Symmorphosis through dietary regulation: a combinatorial role for proteolysis, autophagy and protein synthesis in normalising muscle metabolism and function of hypertrophic mice after acute starvation.
    Authors: Collins-Hooper H, Sartori R, Giallourou N, Matsakas A, Mitchell R, Makarenkova H, Flasskamp H, Macharia R, Ray S, Swann J, Sandri M, Patel K
    PLoS ONE, 2015;10(3):e0120524.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  20. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
    Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
    J Clin Invest, 2015;125(3):1243-54.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Types: Cell Lysates
    Applications: WB
  21. Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and gammadeltaT Cells.
    Authors: Shimizu K, Shinga J, Yamasaki S, Kawamura M, Dorrie J, Schaft N, Sato Y, Iyoda T, Fujii S
    PLoS ONE, 2015;10(6):e0131477.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  22. Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
    Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
    PLoS ONE, 2015;10(4):e0121103.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  23. MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death.
    Authors: Chaudhuri A, Kabaria S, Choi D, Mouradian M, Junn E
    J Biol Chem, 2015;290(19):12425-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  24. Propionibacterium acnes Induces IL-1beta secretion via the NLRP3 inflammasome in human monocytes.
    Authors: Qin M, Pirouz A, Kim M, Krutzik S, Garban H, Kim J
    J Invest Dermatol, 2014;134(2):381-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  25. Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.
    Authors: Wang J, Jenkins S, Lamartiniere C
    BMC Cancer, 2014;14(0):379.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB
  26. Inflammation enhances IL-2 driven differentiation of cytolytic CD4 T cells.
    Authors: Workman A, Jacobs A, Vogel A, Condon S, Brown D
    PLoS ONE, 2014;9(2):e89010.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  27. MicroRNA-7 protects against 1-methyl-4-phenylpyridinium-induced cell death by targeting RelA.
    Authors: Choi D, Chae Y, Kabaria S, Chaudhuri A, Jain M, Li H, Mouradian M, Junn E
    J Neurosci, 2014;34(38):12725-37.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  28. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  29. Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin.
    Authors: Sangokoya, Carolyn, Doss, Jennifer, Chi, Jen-Tsan
    PLoS Genet, 2013;9(4):e1003408.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  30. A fragment of the LG3 peptide of endorepellin is present in the urine of physically active mining workers: a potential marker of physical activity.
    Authors: Parker TJ, Sampson DL, Broszczak D
    PLoS ONE, 2012;7(3):e33714.
    Species: Human
    Sample Types: Urine
    Applications: WB
  31. BMP-3 promotes mesenchymal stem cell proliferation through the TGF-beta/activin signaling pathway.
    Authors: Stewart A, Guan H, Yang K
    J. Cell. Physiol., 2010;223(3):658-66.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  32. The major outer membrane protein of a periodontopathogen induces IFN-beta and IFN-stimulated genes in monocytes via lipid raft and TANK-binding kinase 1/IFN regulatory factor-3.
    Authors: Lee SH, Kim JS, Jun HK
    J. Immunol., 2009;182(9):5823-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  33. Long-term high glucose concentration influences Akt, ERK1/2, and PTP1B protein expression in human aortic smooth muscle cells.
    Authors: Popov D, Nemecz M, Dumitrescu M, Georgescu A, Bohmer FD
    Biochem. Biophys. Res. Commun., 2009;388(1):51-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  34. Hyperoxia modulates TGF-beta/BMP signaling in a mouse model of bronchopulmonary dysplasia.
    Authors: Alejandre-Alcazar MA, Kwapiszewska G, Reiss I, Amarie OV, Marsh LM, Sevilla-Perez J, Wygrecka M, Eul B, Kobrich S, Hesse M, Schermuly RT, Seeger W, Eickelberg O, Morty RE
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(2):L537-49.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  35. The ERK1/2 signaling pathway regulates 11beta-hydroxysteroid dehydrogenase type 2 expression in human trophoblast cells through a transcriptional mechanism.
    Authors: Guan H, Sun K, Yang K
    Biol Reprod, 0;89(4):92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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Reviews for Rabbit IgG HRP-conjugated Antibody

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Rabbit IgG HRP-conjugated Antibody
By Anonymous on 04/24/2018
Application: IHC Sample Tested: Spleen tissue Species: Mouse

Rabbit IgG HRP-conjugated Antibody
By Anonymous on 10/31/2017
Application: ELISA Sample Tested: Human recombinant antibody Species: Human