Rat alpha _2u‑Globulin Biotinylated Antibody

alpha _2u-Globulin in Rat Liver.

a_2u-Globulin was detected in perfusion fixed frozen sections of rat liver using Mouse Anti-Rat a_2u-Globulin Biotinylated Monoclonal Antibody (Catalog # BAM586) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

alpha _2u‑Globulin in Rat Kidney.

alpha _2u‑Globulin was detected in immersion fixed paraffin-embedded sections of rat kidney using Mouse Anti-Rat alpha _2u‑Globulin Biotinylated Monoclonal Antibody (Catalog # BAM586) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.