Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 129736
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Product Specifications

Immunogen

Rat urine-derived alpha 2u-Globulin

Specificity

Detects rat alpha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rat alpha 2u‑Globulin Antibody

a2u-Globulin antibody in Rat Kidney by Immunohistochemistry (IHC-Fr).

alpha 2u‑Globulin in Rat Kidney.

a2u-Globulin was detected in perfusion fixed frozen sections of rat kidney using Mouse Anti-Rat a2u-Globulin Monoclonal Antibody (Catalog # MAB586) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat alpha 2u‑Globulin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat kidney

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha 2u-Globulin

alpha -2u Globulin, also known as PGCL1, is a member of a highly homologous multigene family of more than 20 members that show 87%‑98% amino acid identity. It is expressed at highest levels in male liver and coagulate glands.

Alternate Names

AUG, PGCL1

Additional alpha 2u-Globulin Products

Product Documents for Rat alpha 2u‑Globulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat alpha 2u‑Globulin Antibody

For research use only

Related Research Areas

Citations for Rat alpha 2u‑Globulin Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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