Detection of Rat alpha 1‑Microglobulin by Western Blot.
Western blot shows rat plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat alpha 1‑Microglobulin Monoclonal Antibody (Catalog # MAB7720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for alpha 1‑Microglobulin at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat alpha 1‑Microglobulin by Simple WesternTM.
Simple Western lane view shows rat plasma, loaded at 0.5 mg/mL. A specific band was detected for alpha 1‑Microglobulin at approximately 37 kDa (as indicated) using 20 µg/mL of Mouse Anti-Rat alpha 1‑Microglobulin Monoclonal Antibody (Catalog # MAB7720). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Microglobulin
Rat alpha 1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted 25-26 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. alpha 1‑Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. alpha 1‑Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Rat alpha 1‑Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa alpha 1‑Microglobulin protein (aa 20-202), and a C-terminal serine protease inhibitor termed bikunin (aa 205-349). alpha 1‑Microglobulin possesses one lipocalin domain (aa 41-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 25 kDa alpha 1‑Microglobulin and 28 kDa bikunin molecule, the 55-65 kDa AMBP precursor can also be released intact. In human, alpha 1‑Microglobulin will undergo extracellular processing, generating an isoform that is missing the C-terminal four amino acids. There are four potential isoform variants. One utilizes an alternative start site at Met181, a second contains an Asn substitution for aa 112-228, a third possesses an 11 aa substitution for aa 1-141, and a fourth shows a 17 aa substitution for aa 201-349. Over aa 20-202, rat alpha 1‑Microglobulin shares 76% and 86% aa sequence identity with human and mouse alpha 1‑Microglobulin, respectively
Entrez Gene IDs:
259 (Human); 11699 (Mouse); 25377 (Rat)
A1M; alpha 1Microglobulin; alpha-1-microglobulin/bikunin precursor; AMBP; bikunin; EDC1; HCP; HCPcomplex-forming glycoprotein heterogeneous in charge; HI30; IATIL; inter-alpha-trypsin inhibitor light chain; ITI; ITIL; ITILC; ITILgrowth-inhibiting protein 19; protein AMBP; protein HC; trypstatin; uristatin; uronic-acid-rich protein; UTI
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