Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 771803
View all alpha 1-Microglobulin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat alpha 1‑Microglobulin
Asp20-Ala202
Accession # Q64240

Specificity

Detects rat alpha 1‑Microglobulin in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human alpha 1-Microglobulin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rat alpha 1‑Microglobulin Antibody

Detection of Rat a1-Microglobulin antibody by Western Blot.

Detection of Rat alpha 1‑Microglobulin by Western Blot.

Western blot shows rat plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat a1-Microglobulin Monoclonal Antibody (Catalog # MAB7720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for a1-Microglobulin at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Rat a1-Microglobulin antibody by Simple WesternTM.

Detection of Rat alpha 1‑Microglobulin by Simple WesternTM.

Simple Western lane view shows rat plasma, loaded at 0.5 mg/mL. A specific band was detected for alpha 1‑Microglobulin at approximately 37 kDa (as indicated) using 20 µg/mL of Mouse Anti-Rat alpha 1‑Microglobulin Monoclonal Antibody (Catalog # MAB7720). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Rat alpha 1‑Microglobulin Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Rat plasma

Western Blot

2 µg/mL
Sample: Rat plasma

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha 1-Microglobulin

Rat alpha 1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted 25-26 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. alpha 1‑Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. alpha 1‑Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Rat alpha 1‑Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa alpha 1‑Microglobulin protein (aa 20-202), and a C-terminal serine protease inhibitor termed bikunin (aa 205-349). alpha 1‑Microglobulin possesses one lipocalin domain (aa 41-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 25 kDa alpha 1‑Microglobulin and 28 kDa bikunin molecule, the 55-65 kDa AMBP precursor can also be released intact. In human, alpha 1‑Microglobulin will undergo extracellular processing, generating an isoform that is missing the C-terminal four amino acids. There are four potential isoform variants. One utilizes an alternative start site at Met181, a second contains an Asn substitution for aa 112-228, a third possesses an 11 aa substitution for aa 1-141, and a fourth shows a 17 aa substitution for aa 201-349. Over aa 20-202, rat alpha 1‑Microglobulin shares 76% and 86% aa sequence identity with human and mouse alpha 1‑Microglobulin, respectively

Alternate Names

A1M, alpha 1Microglobulin, AMBP, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI

Entrez Gene IDs

259 (Human); 11699 (Mouse); 25377 (Rat)

Gene Symbol

AMBP

UniProt

Q64240

Additional alpha 1-Microglobulin Products

Product Documents for Rat alpha 1‑Microglobulin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat alpha 1‑Microglobulin Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Inflammatory Mediators

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