Detects rat beta 2-Microglobulin in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant rat alpha 2-Macroglobulin is observed.
Monoclonal Mouse IgM Clone # 747502
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant rat beta 2-Microglobulin
Accession # P07151
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols
are available in the Technical Information section on our website.
Detection of Rat beta 2‑Microglobulin in Rat Splenocytes. Rat splenocytes were stained with Anti-Rat CD3 FITC-conjugated Monoclonal Antibody and either (A) Mouse IgM Isotype control or (B) Mouse Anti-Rat beta 2‑Microglobulin Monoclonal Antibody (Catalog # MAB3864) followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta 2-Microglobulin
beta 2-Microglobulin ( beta 2M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta 2M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta 2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat beta 2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.
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