Rat beta 2-Microglobulin Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat beta 2-Microglobulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat alpha 2-Macroglobulin is observed.
  • Source
    Monoclonal Mouse IgM Clone # 747502
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat beta 2-Microglobulin
    Ile21-Met119
    Accession # P07151
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat beta 2‑Microglobulin in Rat Splenocytes. Rat splenocytes were stained with Anti-Rat CD3 FITC-conjugated Monoclonal Antibody and either (A) Mouse IgM Isotype control or (B) Mouse Anti-Rat beta 2‑Microglobulin Monoclonal Antibody (Catalog # MAB3864) followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta 2-Microglobulin

beta 2-Microglobulin ( beta 2M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta 2M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta 2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat  beta 2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.

  • Entrez Gene IDs:
    567 (Human); 12010 (Mouse); 24223 (Rat)
  • Alternate Names:
    B2M; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
Related Research Areas
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