Rat IL-13 Biotinylated Antibody

    
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  • Species Reactivity
    Rat
  • Specificity
    Detects rat IL-13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL‑4, recombinant human IL-13, rmIL-13, or recombinant rhesus macaque IL-13 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 296202
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant rat IL-13
    Thr19-His131
    Accession # P42203
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Rat IL‑13 (Catalog # 1945-RL)
    under non-reducing conditions only
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunocytochemistry
IL‑13 in Rat Splenocytes. IL‑13 was detected in immersion fixed rat splenocytes treated with PHA using Mouse Anti-Rat IL‑13 Biotinylated Monoclonal Antibody (Catalog # BAM1945) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature rat IL-13 shares 59%, 75%, and 60% amino acid sequence identity with human, mouse, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

  • References:
    1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
    2. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
    3. Lakkis, F.G and E.N. Cruet (1993) Biochem. Biophys. Res. Commun. 197:612.
    4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
    5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
    6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
    7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
    8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
    9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
    10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
    11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
    12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387
    13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun.360:464.
    14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
    15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
    16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
    17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
  • Long Name:
    Interleukin 13
  • Entrez Gene IDs:
    3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
  • Alternate Names:
    ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
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Isotype Controls
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Mouse IgG2B Biotinylated Isotype Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Staining Reagents
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Streptavidin-Phycoerythrin

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Streptavidin-Alkaline Phosphatase

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NorthernLights Streptavidin NL557

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NorthernLights Streptavidin NL493

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Avidin-Fluorescein

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Streptavidin-HRP

ELISA DY998 50  
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EvenCoat Streptavidin Coated Plates, 5 Pack

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