Rat IL-1Rrp2/IL-1R6 Antibody

Catalog # Availability Size / Price Qty
MAB573
MAB573-SP
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Rat IL-1Rrp2/IL-1R6 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL‑1 Rrp2/IL‑1 R6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) IL-1 Rrp2 is observed and no cross-reactivity with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, rhIL-1 RAcP, rmIL-1 R5, rmIL‑1 R7, and rmIL-1 RAPL1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 131011
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat IL‑1 Rrp2/IL‑1 R6
Gly22-Tyr340
Accession # Q62929
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat IL‑1 Rrp2/IL‑1 R6 Fc Chimera (Catalog # 573-RP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1Rrp2/IL-1R6

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL‑18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll‑like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Rat IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 319 aa extracellular domain. It shares 84% and 67% amino acid sequence identity with mouse and human IL‑1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL‑1 Rrp2 has been shown to mediate the activation of the transcription factor NF‑kB by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1e), with IL‑1 RAcP as a co-factor (3, 4). Response to IL-1 F9 is specifically antagonized by IL-1 F5 (also known as IL-1d), an IL-1 family ligand that is most closely related to IL‑1ra (3). IL-1 Rrp2, IL-1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).

References
  1. Boraschi, D. & A. Tagliabue (2006) Vitam. Horm. 74:229.
  2. Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
  3. Debets, R. et al. (2001) J. Immunol. 167:1440.
  4. Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 1 Receptor-like 2
Entrez Gene IDs
8808 (Human); 107527 (Mouse); 171106 (Rat)
Alternate Names
IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2; IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2; interleukin 1 receptor-like 2; interleukin-1 receptor-like 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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