Detects rat CXCL5/LIX in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse GCP-2 is observed and less than 5% cross‑reactivity with recombinant human GCP-2 is observed and no cross reactivity with _____________ is observed.
Polyclonal Goat IgG
E. coli-derived recombinant rat LIX Ala38-Gln130 Accession # P97885.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize CXCL5/LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.2 µg/mL Recombinant Rat CXCL5/LIX.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL5/LIX and Neutralization by Rat CXCL5/LIX Antibody. Recombinant Rat CXCL5/LIX (Catalog # 543-RL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL5/LIX (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL5/LIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF543). The ND50 is typically 0.3-1.5 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LPS-induced CXC chemokine (LIX) is a member of the murine chemokine family. It is 132 amino acids (aa) in length with a predicted molecular weight of 14 kDa prior to cleavage of the N-terminal signal peptide (aa 1–40). LIX has no true human ortholog but is closely related to human CXCL5/ENA-78 and CXCL6/GCP-2. It is widely expressed, and is secreted by multiple cell types including fibroblasts, thymic epithelium, platelets, vascular endothelium, and hepatocytes. LIX acts as a proinflammatory chemokine and is chemotactic for neutrophils.
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