Rat MuSK Antibody

(7 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat MuSK in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat MuSK
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat MuSK
  • Neutralization
    Measured by its ability to neutralize MuSK-dependent acetylcholine receptor clustering on myotubes differentiated from the C2C12 mouse myoblast cell line [Ferns, M.J. et al. (1993) Neuron 11:491]. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 16 ng/mL Recombinant Rat Agrin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Acetylcholine Receptor Clustering Mediated by MuSK and Neutralization by Rat MuSK Antibody. Recombinant Rat Agrin (Catalog # 550-AG) induces MuSK-dependent acetylcholine receptor clustering on myotubes differentiated from the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Acetylcholine Receptor Clustering elicited by Recombinant Rat Agrin (16 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat MuSK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF562). The ND50 is typically 0.5-2 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MuSK
MuSK (Muscle-Specific Kinase) is a receptor tyrosine kinase expressed in muscle that regulates the formation of the neuromuscular junction. It binds the heparin sulfate proteoglycan Agrin to promote Acetylcholine Receptor clustering.
  • Long Name:
    Muscle-specific Receptor Tyrosine Kinase
  • Entrez Gene IDs:
    4593 (Human); 81725 (Rat)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; MGC126323; MGC126324; muscle, skeletal, receptor tyrosine kinase; MuSK; skeletal receptor tyrosine-protein kinase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. DOK7 gene therapy enhances motor activity and life span in ALS model mice
    Authors: S Miyoshi, T Tezuka, S Arimura, T Tomono, T Okada, Y Yamanashi
    EMBO Mol Med, 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. MuSK myasthenia gravis IgG4 disrupts the interaction of LRP4 with MuSK but both IgG4 and IgG1-3 can disperse preformed agrin-independent AChR clusters.
    Authors: Koneczny, Inga, Cossins, Judith, Waters, Patrick, Beeson, David, Vincent, Angela
    PLoS ONE, 2013;8(11):e80695.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  3. Immunocapture and identification of cell membrane protein antigenic targets of serum autoantibodies.
    Authors: Littleton E, Dreger M, Palace J
    Mol. Cell Proteomics, 2009;8(7):1688-96.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Mutations causing DOK7 congenital myasthenia ablate functional motifs in Dok-7.
    Authors: Hamuro J, Higuchi O, Okada K, Ueno M, Iemura S, Natsume T, Spearman H, Beeson D, Yamanashi Y
    J. Biol. Chem., 2007;283(9):5518-24.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  5. MuSK expressed in the brain mediates cholinergic responses, synaptic plasticity, and memory formation.
    Authors: Garcia-Osta A, Tsokas P, Pollonini G, Landau EM, Blitzer R, Alberini CM
    J. Neurosci., 2006;26(30):7919-32.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. 14-3-3 gamma associates with muscle specific kinase and regulates synaptic gene transcription at vertebrate neuromuscular synapse.
    Authors: Strochlic L, Cartaud A, Mejat A, Grailhe R, Schaeffer L, Changeux JP, Cartaud J
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(52):18189-94.
    Species: Rat
    Sample Type: Cell Lysates
    Application: IP
  7. Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction.
    Authors: Finn AJ, Feng G, Pendergast AM
    Nat. Neurosci., 2003;6(7):717-23.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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