Rat Oncomodulin Antibody

Catalog # Availability Size / Price Qty
Detection of Rat Oncomodulin by Western Blot.
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Product Details
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Rat Oncomodulin Antibody Summary

Species Reactivity
Detects rat Oncomodulin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Oncomodulin is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant rat Oncomodulin
Accession # P02631
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat Oncomodulin antibody by Western Blot. View Larger

Detection of Rat Oncomodulin by Western Blot. Western blot shows lysates of rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Rat Oncomodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6345) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Oncomodulin at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oncomodulin

Oncomodulin (OM; also parvalbumin beta) is a 12 - 14 kDa member of the parvalbumin family of Ca++binding proteins. It is expressed in early embryonic cells, placenta, and in tumors. OM was originally thought to have expression restricted to neoplastic tissues, early embryonic cells and certain tumor cell lines. Recent research shows that oncomodulin is also expressed and secreted by macrophages where, in the retina, it binds to retinal ganglion cells (RGCs) and functions to promote axon regenerationin early embryonic cells, placenta, and in tumors. OM is both cytoplasmic, and secreted. Rat OM is 109 amino acids (aa) in length. It contains a vestigial Ca++binding site (aa 733) and two EF hand domains, the latter of which contains one highaffinity Ca++binding site (aa 78108). Relative to parvalbumin alpha, OM has a lower pI (<4.8), a higher affinity for Ca++, and they share only 50% aa identity. Full length rat OM shares 95% and 89% aa identity with mouse and human OM, respectively.

Entrez Gene IDs
654231 (Human); 18261 (Mouse); 25503 (Rat)
Alternate Names
OCM; OCM1; OCMN; OM; ONCM; Oncomodulin; Parvalbumin beta

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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