Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Human, Mouse, Rat, Transgenic Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant rat Reg3B
Glu27-Gly175
Accession # P25031

Specificity

Detects rat Reg3B in direct ELISAs and Western blots. In direct ELISAs, approxmately 75% cross-reactivity with recombinant mouse (rm) Reg3B, and approximately 20% cross-reactivity with rmReg3G is observed, and less than 2% cross-reactivity with recombinant human (rh) Reg3A, rmReg1, rmReg2, rhReg4, rhReg1A, rmReg3A, and rmReg3D is observed. In Western blots, approximately 40% cross-reactivity with rmReg3B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Rat Reg3B Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat Reg3B

Reviewed Applications

Read 2 reviews rated 4 using AF1996 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Reg3B

Rat Reg3B, also named pancreatitis-associated protein 1, Regenerating islet-derived protein 3-beta and peptide 23, belongs to the Regenerating gene family (Reg), within the superfamily of C-type lectins. It has been classified as a member of the type III subclass of the Reg family. Rat Reg3B has anti-apoptotic effects on pancreatic acinar cells and is a novel motor and sensory neuron survival factor.

Long Name

Regenerating Islet-derived IIIb

Alternate Names

PAP1

Entrez Gene IDs

18489 (Mouse); 24618 (Rat)

Gene Symbol

REG3B

UniProt

Additional Reg3B Products

Product Documents for Rat Reg3B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Reg3B Antibody

For research use only

Related Research Areas

Citations for Rat Reg3B Antibody

Customer Reviews for Rat Reg3B Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 2 of 2 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21410322
    Species: Mouse
    Verified Customer | Posted 01/06/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23147030
    Species: Mouse
    Verified Customer | Posted 01/06/2015

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