Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Human, Mouse, Rat, Transgenic Rat
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot, In vivo assay
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant rat Reg3B
Glu27-Gly175
Accession # P25031
Glu27-Gly175
Accession # P25031
Specificity
Detects rat Reg3B in direct ELISAs and Western blots. In direct ELISAs, approxmately 75% cross-reactivity with recombinant mouse (rm) Reg3B, and approximately 20% cross-reactivity with rmReg3G is observed, and less than 2% cross-reactivity with recombinant human (rh) Reg3A, rmReg1, rmReg2, rhReg4, rhReg1A, rmReg3A, and rmReg3D is observed. In Western blots, approximately 40% cross-reactivity with rmReg3B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Rat Reg3B Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Rat Reg3B
Sample: Recombinant Rat Reg3B
Reviewed Applications
Read 2 reviews rated 4 using AF1996 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Reg3B
Long Name
Regenerating Islet-derived IIIb
Alternate Names
PAP1
Gene Symbol
REG3B
UniProt
Additional Reg3B Products
Product Documents for Rat Reg3B Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Rat Reg3B Antibody
For research use only
Related Research Areas
Citations for Rat Reg3B Antibody
Customer Reviews for Rat Reg3B Antibody (2)
4 out of 5
2 Customer Ratings
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Application: Western BlotSample Tested: See PMID 21410322Species: MouseVerified Customer | Posted 01/06/2015
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Application: Western BlotSample Tested: See PMID 23147030Species: MouseVerified Customer | Posted 01/06/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars