Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 559706
View all RTN1-A/NSP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat RTN1-A/NSP
Met73-Ser502
Accession # Q64548

Specificity

Detects rat RTN1-A/NSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat RTN-A2B, recombinant human (rh) RTN1-A, and rhRTN3-A1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Rat RTN1‑A/NSP Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cerebellum)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RTN1-A/NSP

Reticulon-1 (RTN1-A/RTN1) is a 135 kDa multi-pass membrane protein localized to the endoplasmic reticulum of neurons. RTN1-A belongs to no known family of proteins. Rat RTN1-A is 777 amino acids (aa) in length and contains two transmembrane segments and a Reticulon domain (aa 592-759). There are also multiple potential sites for phosphorylation. Alternative splicing produces a second isoform known as RTN1-S, which is missing residues corresponding to aa 1-569 in RTN1-A, and has a 20 aa substitution for residues corresponding to aa 570-589. Rat RTN1-A is 82% aa identical to human RTN1-A

Long Name

Reticulon 1A/Neuroendocrine-specific Protein

Alternate Names

NSP-A, RTN1, RTN1A

Entrez Gene IDs

6252 (Human); 116644 (Rat)

Gene Symbol

RTN1

UniProt

Q64548

Additional RTN1-A/NSP Products

Product Documents for Rat RTN1‑A/NSP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat RTN1‑A/NSP Antibody

For research use only

Related Research Areas

Neuronal Markers

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Protocols

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