hh Rat TNF-alpha Quantikine ELISA Kit RTA00: R&D Systems

Rat TNF-alpha Quantikine ELISA Kit

(93 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Specificity
    Natural and recombinant rat TNF-alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat TNF-alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat TNF-alpha accurately. Results obtained using natural rat TNF-alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat TNF-alpha.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean6523259363246656
Standard Deviation3.35.112.56.123.657.6
CV%5.12.22.19.79.68.8

Recovery

The recovery of rat TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 101 93-119
EDTA Plasma (n=5) 96 88-104
Heparin Plasma (n=4) 80 73-84
Serum (n=8) 93 83-105
Linearity
To assess the linearity of the assay, samples spiked with various concentrations of rat TNF-alpha were diluted with Calibrator Diluent and then assayed. Results from typical sample dilutions are shown.
Rat TNF-alpha Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TNF-alpha

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

    • Long Name
      Tumor Necrosis Factor alpha
    • Entrez Gene IDs
      7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit);
    • Alternate Names
      APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    93 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Pharmacologic targeting ERK1/2 attenuates the development and progression of hyperuricemic nephropathy in rats
      Authors: N Liu, L Xu, Y Shi, L Fang, H Gu, H Wang, X Ding, S Zhuang
      Oncotarget, 2017;8(20):33807-33826.
      Species: Rat
      Sample Type: Tissue Culture Supernates
    2. The Effects of Remifentanil on Expression of High Mobility Group Box 1 in Septic Rats
      Authors: KH Seo, JW Choi, HS Jung, H Yoo, JD Joo
      J. Korean Med. Sci, 2017;32(3):542-551.
      Species: Rat
      Sample Type: Serum
    3. Activated Microglia Desialylate and Phagocytose Cells via Neuraminidase, Galectin-3, and Mer Tyrosine Kinase
      Authors: K Nomura, A Vilalta, DH Allendorf, TC Hornik, GC Brown
      J. Immunol., 2017;0(0):.
      Species: Rat
      Sample Type: Cell Culture Supernates
    4. Sodium tanshinone IIA sulfonate improves inflammation, aortic endothelial cell apoptosis, disseminated intravascular coagulation and multiple organ damage in a rat heat stroke model
      Authors: F Chen, H Li, G Zhu, X Chen, Z Tang
      Mol Med Rep, 2017;0(0):.
      Species: Rat
      Sample Type: Serum
    5. Effects of TNF-? on penile structure alteration in rats with hyperprolactinemia
      Authors: ZL Wang, LY Yang, HH Chen, HH Lin, YT Tsai, WJ Huang
      PLoS ONE, 2017;12(8):e0181952.
      Species: Rat
      Sample Type: Serum
    6. Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis
      Authors: S Xie, G Li, L Qu, R Zhong, P Chen, Z Lu, J Zhou, X Guo, Z Li, A Ma, Y Qian, Y Zhu
      Evid Based Complement Alternat Med, 2017;2017(0):6958982.
      Species: Rat
      Sample Type: Cell Culture Supernates
    7. Oral Administration of Red Ginseng Extract Promotes Neurorestoration after Compressive Spinal Cord Injury in Rats
      Authors: P Zhu, K Samukawa, H Fujita, H Kato, M Sakanaka
      Evid Based Complement Alternat Med, 2017;2017(0):1265464.
      Species: Rat
      Sample Type: Tissue Homogenates
    8. Chronic Cognitive Dysfunction after Traumatic Brain Injury Is Improved with a Phosphodiesterase 4B Inhibitor
      J Neurosci, 2016;36(27):7095-108.
      Species: Rat
      Sample Type: Tissue Homogenates
    9. Melatonin attenuates the TLR4-mediated inflammatory response through MyD88- and TRIF-dependent signaling pathways in an in vivo model of ovarian cancer.
      Authors: Chuffa L, Fioruci-Fontanelli B, Mendes L, Ferreira Seiva F, Martinez M, Favaro W, Domeniconi R, Pinheiro P, Delazari Dos Santos L, Martinez F
      BMC Cancer, 2015;15(0):34.
      Species: Rat
      Sample Type: Tissue Culture Supernates
    10. Ameliorative effect of allopurinol on vascular complications of insulin resistance.
      Authors: El-Bassossy H, Elberry A, Azhar A, Ghareib S, Alahdal A
      J Diabetes Res, 2015;2015(0):178540.
      Species: Rat
      Sample Type: Serum
    11. Co-Administration of Cholesterol-Lowering Probiotics and Anthraquinone from Cassia obtusifolia L. Ameliorate Non-Alcoholic Fatty Liver.
      Authors: Mei L, Tang Y, Li M, Yang P, Liu Z, Yuan J, Zheng P
      PLoS ONE, 2015;10(9):e0138078.
      Species: Rat
      Sample Type: Serum
    12. IL-17A is implicated in lipopolysaccharide-induced neuroinflammation and cognitive impairment in aged rats via microglial activation.
      Authors: Sun J, Zhang S, Zhang X, Zhang X, Dong H, Qian Y
      J Neuroinflammation, 2015;12(0):165.
      Species: Rat
      Sample Type: Serum
    13. Xanthine oxidase inhibition alleviates the cardiac complications of insulin resistance: effect on low grade inflammation and the angiotensin system.
      Authors: El-Bassossy H, Watson M
      J Transl Med, 2015;13(0):82.
      Species: Rat
      Sample Type: Serum
    14. Non-invasive technology that improves cardiac function after experimental myocardial infarction: Whole Body Periodic Acceleration (pGz).
      Authors: Uryash A, Bassuk J, Kurlansky P, Altamirano F, Lopez J, Adams J
      PLoS ONE, 2015;10(3):e0121069.
      Species: Rat
      Sample Type: Tissue Homogenates
    15. The extent of irradiation-induced long-term visceral organ damage depends on cranial/brain exposure.
      Authors: Boittin F, Denis J, Mayol J, Martigne P, Raffin F, Coulon D, Grenier N, Drouet M, Herodin F
      PLoS ONE, 2015;10(4):e0122900.
      Species: Rat
      Sample Type: Plasma
    16. Rhamnogalacturonan from Acmella oleracea (L.) R.K. Jansen: gastroprotective and ulcer healing properties in rats.
      Authors: Maria-Ferreira D, da Silva L, Mendes D, Cabrini D, Nascimento A, Iacomini M, Cipriani T, Santos A, Werner M, Baggio C
      PLoS ONE, 2014;9(1):e84762.
      Species: Rat
      Sample Type: Tissue Culture Supernates
    17. Epidermal growth factor receptor inhibitor ameliorates excessive astrogliosis and improves the regeneration microenvironment and functional recovery in adult rats following spinal cord injury.
      Authors: Li Z, Li J, Wang L, Zhang J, Wu J, Mao X, Shi G, Wang Q, Wang F, Zou J
      J Neuroinflammation, 2014;11(0):71.
      Species: Rat
      Sample Type: Cell Culture Supernates
    18. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
      Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
      BMC Biotechnol, 2014;14(0):63.
      Species: Rat
      Sample Type: Serum
    19. Deoxyglucose prevents neurodegeneration in culture by eliminating microglia.
      Authors: Vilalta, Anna, Brown, Guy C
      J Neuroinflammation, 2014;11(0):58.
      Species: Rat
      Sample Type: Cell Culture Supernates
    20. Catalpol ameliorates sodium taurocholate-induced acute pancreatitis in rats via inhibiting activation of nuclear factor kappa B.
      Authors: Xiao W, Yin G, Fan Y, Qiu L, Cang X, Yu G, Hu Y, Xing M, Wu D, Wang X, Hu G, Wan R
      Int J Mol Sci, 2014;15(7):11957-72.
      Species: Rat
      Sample Type: Serum
    21. Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis.
      Authors: Liu C, Hong T, Shao M, Chen Z, Wang C
      Mol Med Rep, 2014;10(3):1323-8.
      Species: Rat
      Sample Type: Serum
    22. Dose responsive effects of subcutaneous pentosan polysulfate injection in mucopolysaccharidosis type VI rats and comparison to oral treatment.
      Authors: Frohbergh M, Ge Y, Meng F, Karabul N, Solyom A, Lai A, Iatridis J, Schuchman E, Simonaro C
      PLoS ONE, 2014;9(6):e100882.
      Species: Rat
      Sample Type: Serum
    23. Pentoxifylline alleviates cardiac ischemia and dysfunction following experimental angina in insulin resistance.
      Authors: Azhar A, El-Bassossy H
      PLoS ONE, 2014;9(5):e98281.
      Species: Rat
      Sample Type: Serum
    24. Preventive effects of probiotic bacteria Lactobacillus plantarum and dietary fiber in chemically-induced mammary carcinogenesis.
      Authors: Kassayova M, Bobrov N, Strojny L, Kiskova T, Mikes J, Demeckova V, Orendas P, Bojkova B, Pec M, Kubatka P, Bomba A
      Anticancer Res, 2014;34(9):4969-75.
      Species: Rat
      Sample Type: Serum
    25. A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways, with no additional protection from prolonged infusion.
      Cytokine, 2013;61(2):614-21.
      Species: Rat
      Sample Type: BALF
    26. Caspase inhibitors protect neurons by enabling selective necroptosis of inflamed microglia.
      Authors: Fricker M, Vilalta A, Tolkovsky A, Brown G
      J Biol Chem, 2013;288(13):9145-52.
      Species: Rat
      Sample Type: Cell Culture Supernates
    27. Cerebral ischemia is exacerbated by extracellular nicotinamide phosphoribosyltransferase via a non-enzymatic mechanism.
      Authors: Zhao B, Zhang M, Han X, Zhang X, Xing Q, Dong X, Shi Q, Huang P, Lu Y, Wei E, Xia Q, Zhang W, Tang C
      PLoS ONE, 2013;8(12):e85403.
      Species: Rat
      Sample Type: Cell Culture Supernates
    28. Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-alpha production in RBL-2H3 cells.
      Authors: Yoshioka Y, Ye Y, Okada K, Taniguchi K, Yoshida A, Sugaya K, Onose J, Koshino H, Takahashi S, Yajima A, Yajima S, Abe N
      PLoS ONE, 2013;8(12):e80931.
      Species: Rat
      Sample Type: Whole Cells
    29. Suppression of neuroinflammatory and apoptotic signaling cascade by curcumin alone and in combination with piperine in rat model of olfactory bulbectomy induced depression.
      Authors: Rinwa P, Kumar A, Garg S
      PLoS ONE, 2013;8(4):e61052.
      Species: Rat
      Sample Type: Tissue Homogenates
    30. Medium-chain fatty acid-sensing receptor, GPR84, is a proinflammatory receptor.
      Authors: Suzuki M, Takaishi S, Nagasaki M, Onozawa Y, Iino I, Maeda H, Komai T, Oda T
      J Biol Chem, 2013;288(15):10684-91.
      Species: Rat
      Sample Type: Serum
    31. Positional isomers of aspirin are equally potent in inhibiting colon cancer cell growth: differences in mode of cyclooxygenase inhibition.
      Authors: Kodela R, Chattopadhyay M, Goswami S, Gan Z, Rao P, Nia K, Velazquez-Martinez C, Kashfi K
      J Pharmacol Exp Ther, 2013;345(1):85-94.
      Species: Rat
      Sample Type: Plasma
    32. Mechanical ventilation enhances HMGB1 expression in an LPS-induced lung injury model.
      Authors: Ding N, Wang F, Xiao H, Xu L, She S
      PLoS ONE, 2013;8(9):e74633.
      Species: Rat
      Sample Type: BALF
    33. The effects of low-dose ionizing radiation in the activated rat basophilic leukemia (RBL-2H3) mast cells.
      Authors: Joo H, Nam S, Yang K, Kim C, Jin Y, Kim J
      J Biol Chem, 2012;287(33):27789-95.
      Species: Rat
      Sample Type: Cell Culture Supernates
    34. Anti-inflammatory effects of FTY720 do not prevent neuronal cell loss in a rat model of optic neuritis.
      Authors: Rau CR, Hein K, Sattler MB, Kretzschmar B, Hillgruber C, McRae BL, Diem R, Bahr M
      Am. J. Pathol., 2012;178(4):1770-81.
      Species: Rat
      Sample Type: Tissue Homogenates
    35. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
      J Neuroinflammation, 2012;9(0):181.
      Species: Rat
      Sample Type: Tissue Homogenates
    36. Glial tumor necrosis factor alpha (TNF-alpha) generates metaplastic inhibition of spinal learning.
      Authors: Huie JR, Baumbauer KM, Lee KH, Bresnahan JC, Beattie MS, Ferguson AR, Grau JW
      PLoS ONE, 2012;7(6):e39751.
      Species: Rat
      Sample Type: Tissue Homogenates
    37. Vipera aspis venom reduces lethality and down-regulates tumor necrosis factor-alpha in a rat model of LPS-induced sepsis.
      Authors: Frolkis I, Klein Y, Locker C, Adi N, Dahan E, Uretzsky G, Shapira I, Sorkine P
      Cytokine, 2010;49(3):319-24.
      Species: Rat
      Sample Type: Serum
    38. Systemic effects of ulna loading in male rats during functional adaptation.
      Authors: Sample SJ, Collins RJ, Wilson AP, Racette MA, Behan M, Markel MD, Kalscheur VL, Hao Z, Muir P
      J. Bone Miner. Res., 2010;25(9):2016-28.
      Species: Rat
      Sample Type: Plasma
    39. House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
      Authors: De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA
      Eur. Respir. J., 2010;35(6):1377-87.
      Species: Rat
      Sample Type: BALF
    40. Hypercapnic acidosis attenuates shock and lung injury in early and prolonged systemic sepsis.
      Authors: Costello J, Higgins B, Contreras M, Chonghaile MN, Hassett P, O'Toole D, Laffey JG
      Crit. Care Med., 2009;37(8):2412-20.
      Species: Rat
      Sample Type: BALF
    41. In vivo Angiotensin II AT1 receptor blockade selectively inhibits LPS-induced innate immune response and ACTH release in rat pituitary gland.
      Authors: Sanchez-Lemus E, Benicky J, Pavel J, Saavedra JM
      Brain Behav. Immun., 2009;23(7):945-57.
    42. Role of sensory innervation in the rat pulmonary neutrophil recruitment induced by staphylococcal enterotoxins type A and B.
      Authors: Desouza IA, Camargo EA, Mariano NS, Optiz-Neto JB, Resende JS, Mello GC, Costa SK, De Nucci G, Antunes E
      Eur. J. Pharmacol., 2009;613(1):128-34.
      Species: Rat
      Sample Type: Cell Culture Supernates
    43. 4-thiouridine induces dose-dependent reduction of oedema, leucocyte influx and tumour necrosis factor in lung inflammation.
      Authors: Evaldsson C, Ryden I, Rosen A, Uppugunduri S
      Clin. Exp. Immunol., 2009;155(2):330-8.
      Species: Rat
      Sample Type: BALF
    44. Effects of an antagonist of the gastrin-releasing peptide receptor in an animal model of uveitis.
      Authors: Pereira DV, Steckert AV, Mina F, Petronilho F, Roesler R, Schwartsmann G, Ritter C, Dal-Pizzol F
      Invest. Ophthalmol. Vis. Sci., 2009;50(11):5300-3.
      Species: Rat
      Sample Type: Aqueous humor
    45. Tumor necrosis factor-alpha participates in apoptosis in the limbic system after myocardial infarction.
      Authors: Kaloustian S, Bah TM, Rondeau I, Mathieu S, Lada-Moldovan L, Ryvlin P, Godbout R, Rousseau G
      Apoptosis, 2009;0(0):.
      Species: Rat
      Sample Type: Plasma
    46. Protease-activated receptor 2 blocking peptide counteracts endotoxin-induced inflammation and coagulation and ameliorates renal fibrin deposition in a rat model of acute renal failure.
      Authors: Jesmin S, Gando S, Zaedi S, Prodhan SH, Sawamura A, Miyauchi T, Hiroe M, Yamaguchi N
      Shock, 2009;32(6):626-32.
      Species: Rat
      Sample Type: Plasma
    47. Mechanism of hepatoprotection in proestrus female rats following trauma-hemorrhage: heme oxygenase-1-derived normalization of hepatic inflammatory responses.
      Authors: Yang S, Hu S, Chen J, Choudhry MA, Rue LW, Bland KI, Chaudry IH
      J. Leukoc. Biol., 2009;85(6):1015-26.
      Species: Rat
      Sample Type: Serum
    48. Effects of the immunomodulator, VGX-1027, in endotoxin-induced uveitis in Lewis rats.
      Authors: Mangano K, Sardesai NY, Quattrocchi C, Mazzon E, Cuzzocrea S, Bendtzen K, Meroni PL, Kim JJ, Nicoletti F
      Br. J. Pharmacol., 2008;155(5):722-30.
      Species: Rat
      Sample Type: Aqueous humor
    49. Role of central beta-adrenergic receptors in regulating proinflammatory cytokine responses to a peripheral bacterial challenge.
      Authors: Johnson JD, Cortez V, Kennedy SL, Foley TE, Hanson H, Fleshner M
      Brain Behav. Immun., 2008;22(7):1078-86.
      Species: Rat
      Sample Type: Plasma
    50. Antithrombin inhibits bronchoalveolar activation of coagulation and limits lung injury during Streptococcus pneumoniae pneumonia in rats.
      Authors: Choi G, Hofstra JJ, Roelofs JJ, Rijneveld AW, Bresser P, van der Zee JS, Florquin S, van der Poll T, Levi M, Schultz MJ
      Crit. Care Med., 2008;36(1):204-10.
      Species: Rat
      Sample Type: BALF
    51. Substance P acts via the neurokinin receptor 1 to elicit bronchoconstriction, oxidative stress, and upregulated ICAM-1 expression after oil smoke exposure.
      Authors: Li PC, Chen WC, Chang LC, Lin SC
      Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(5):L912-20.
      Species: Rat
      Sample Type: BALF
    52. Triamcinolone acetonide protects the rat retina from STZ-induced acute inflammation and early vascular leakage.
      Authors: Kim YH, Choi MY, Kim YS, Park CH, Lee JH, Chung IY, Yoo JM, Choi WS, Cho GJ, Kang SS
      Life Sci., 2007;81(14):1167-73.
      Species: Rat
      Sample Type: Tissue Homogenates
    53. Anti-rat soluble IL-6 receptor antibody down-regulates cardiac IL-6 and improves cardiac function following trauma-hemorrhage.
      Authors: Yang S, Hu S, Choudhry MA, Rue LW, Bland KI, Chaudry IH
      J. Mol. Cell. Cardiol., 2007;42(3):620-30.
      Species: Rat
      Sample Type: Plasma
    54. Interleukin-6 mediates low-threshold mechanical allodynia induced by intrathecal HIV-1 envelope glycoprotein gp120.
      Authors: Schoeniger-Skinner DK, Ledeboer A, Frank MG, Milligan ED, Poole S, Martin D, Maier SF, Watkins LR
      Brain Behav. Immun., 2007;21(5):660-7.
      Species: Rat
      Sample Type: CSF
    55. Role of sex steroids in modulating tumor necrosis factor alpha-induced changes in vascular function and blood pressure.
      Authors: LaMarca BD, Chandler DL, Grubbs L, Bain J, McLemore GR, Granger JP, Ryan MJ
      Am. J. Hypertens., 2007;20(11):1216-21.
      Species: Rat
      Sample Type: Serum
    56. Changes of gene expression of iron regulatory proteins during turpentine oil-induced acute-phase response in the rat.
      Authors: Sheikh N, Dudas J, Ramadori G
      Lab. Invest., 2007;87(7):713-25.
      Species: Rat
      Sample Type: Serum
    57. Prothymosin alpha lacking the nuclear localization signal as an effective gene therapeutic strategy in collagen-induced arthritis.
      Authors: Shiau AL, Chen SY, Chang MY
      J. Immunol., 2007;178(7):4688-94.
      Species: Rat
      Sample Type: Tissue Homogenates
    58. Expression of the mu opioid receptor in the human immunodeficiency virus type 1 transgenic rat model.
      Authors: Chang SL, Beltran JA, Swarup S
      J. Virol., 2007;81(16):8406-11.
      Species: Rat
      Sample Type: Serum
    59. Bacterial translocation is downregulated by anti-TNF-alpha monoclonal antibody administration in rats with cirrhosis and ascites.
      Authors: Frances R, Chiva M, Sanchez E, Gonzalez-Navajas JM, Llovet T, Zapater P, Soriano G, Munoz C, Balanzo J, Perez-Mateo M, Song XY, Guarner C, Such J
      J. Hepatol., 2007;46(5):797-803.
      Species: Rat
      Sample Type: Serum
    60. Cell-based angiopoietin-1 gene therapy for acute lung injury.
      Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
      Am. J. Respir. Crit. Care Med., 2007;175(10):1014-26.
      Species: Rat
      Sample Type: BALF
    61. Natural anticoagulants limit lipopolysaccharide-induced pulmonary coagulation but not inflammation.
      Authors: Choi G, Vlaar AP, Schouten M, Van't Veer C, van der Poll T, Levi M, Schultz MJ
      Eur. Respir. J., 2007;30(3):423-8.
      Species: Rat
      Sample Type: Tissue Homogenates
    62. Comprehensive gene expression profiling of rat lung reveals distinct acute and chronic responses to cigarette smoke inhalation.
      Authors: Stevenson CS, Docx C, Webster R, Battram C, Hynx D, Giddings J, Cooper PR, Chakravarty P, Rahman I, Marwick JA, Kirkham PA, Charman C, Richardson DL, Nirmala NR, Whittaker P, Butler K
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(5):L1183-93.
      Species: Rat
      Sample Type: BALF
    63. 5-aminosalicylic acid improves indomethacin-induced enteropathy by inhibiting iNOS transcription in rats.
      Authors: Nandi J, Saud B, Zinkievich JM, Palma DT, Levine RA
      Dig. Dis. Sci., 2007;53(1):123-32.
      Species: Rat
      Sample Type: Serum
    64. The arthritis severity quantitative trait loci Cia4 and Cia6 regulate neutrophil migration into inflammatory sites and levels of TNF-alpha and nitric oxide.
      Authors: Laragione T, Yarlett NC, Brenner M, Mello A, Sherry B, Miller EJ, Metz CN, Gulko PS
      J. Immunol., 2007;178(4):2344-51.
      Species: Rat
      Sample Type: Air Pouch Exudate
    65. In the adult mesenchymal stem cell population, source gender is a biologically relevant aspect of protective power.
      Authors: Crisostomo PR, Markel TA, Wang M, Lahm T, Lillemoe KD, Meldrum DR
      Surgery, 2007;142(2):215-21.
      Species: Rat
      Sample Type: Cell Culture Supernates
    66. Resuscitation from experimental heatstroke by estrogen therapy.
      Authors: Chen SH, Chang FM, Niu KC, Lin MY, Lin MT
      Crit. Care Med., 2006;34(4):1113-8.
      Species: Rat
      Sample Type: Serum
    67. Glucosamine administration during resuscitation improves organ function after trauma hemorrhage.
      Authors: Yang S, Zou LY, Bounelis P, Chaudry I, Chatham JC, Marchase RB
      Shock, 2006;25(6):600-7.
      Species: Rat
      Sample Type: Serum
    68. Effects of gabexate mesilate on serum inflammatory cytokines in rats with acute necrotizing pancreatitis.
      Authors: Chen CC, Wang SS, Tsay SH, Lee FY, Lu RH, Chang FY, Lee SD
      Cytokine, 2006;33(2):95-9.
      Species: Rat
      Sample Type: Serum
    69. Doxycycline reduces mortality and injury to the brain and cochlea in experimental pneumococcal meningitis.
      Authors: Meli DN, Coimbra RS, Erhart DG, Loquet G, Bellac CL, Tauber MG, Neumann U, Leib SL
      Infect. Immun., 2006;74(7):3890-6.
      Species: Rat
      Sample Type: CSF
    70. P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins.
      Authors: Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P
      J. Biol. Chem., 2006;281(41):30684-96.
      Species: Rat
      Sample Type: Cell Culture Supernates
    71. Anti-inflammatory effects of inhaled nitric oxide are optimized at lower oxygen concentration in experimental Klebsiella pneumoniae pneumonia.
      Authors: Sun Z, Sun B, Wang X, Wang W, Zhu L
      Inflamm. Res., 2006;55(10):430-40.
      Species: Rat
      Sample Type: Tissue Homogenates
    72. Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure.
      Authors: Holly MK, Dear JW, Hu X, Schechter AN, Gladwin MT, Hewitt SM, Yuen PS, Star RA
      Kidney Int., 2006;70(3):496-506.
      Species: Rat
      Sample Type: Serum
    73. Effects of pyrrolidine dithiocarbamate, an NF-kappaB inhibitor, on cytokine expression and ocular inflammation in experimental autoimmune anterior uveitis.
      Authors: Fang IM, Yang CH, Lin CP, Yang CM, Chen MS
      J Ocul Pharmacol Ther, 2005;21(2):95-106.
      Species: Rat
      Sample Type: Aqueous humor
    74. Developmental changes induced by graded prenatal systemic hypoxic-ischemic insults in rats.
      Authors: Robinson S, Petelenz K, Li Q, Cohen ML, Dechant A, Tabrizi N, Bucek M, Lust D, Miller RH
      Neurobiol. Dis., 2005;18(3):568-81.
      Species: Rat
      Sample Type: Tissue Homogenates
    75. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
      Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
      Crit. Care Med., 2005;33(10):2317-22.
      Species: Rat
      Sample Type: BALF
    76. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
      Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
      J. Immunol., 2005;174(4):2327-35.
      Species: Rat
      Sample Type: Cell Culture Supernates
    77. Prophylactic adenovirus-mediated human kallistatin gene therapy suppresses rat arthritis by inhibiting angiogenesis and inflammation.
      Authors: Wang CR, Chen SY, Wu CL, Liu MF, Jin YT, Chao L, Chao J
      Arthritis Rheum., 2005;52(4):1319-24.
      Species: Rat
      Sample Type: Tissue Homogenates
    78. A method for measuring multiple cytokines from small samples.
      Authors: O&amp;amp;apos;Connor KA
      Brain Behav. Immun., 2004;18(3):274-80.
      Species: Rat
      Sample Type: CSF
    79. Ameliorating effects of the immunomodulator 3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole in an experimental model of colitis in the rat.
      Authors: Stasi MA, Ruggiero V, Ursillo A, Taurelli R, Agliano M, Weber E, Lorenzoni P, Sorrentino V, Pacifici L, Carminati P
      Eur. J. Pharmacol., 2004;494(2):263-72.
      Species: Rat
      Sample Type: Tissue Homogenates
    80. Tumor necrosis factor alpha expression and protein levels after fluid percussion injury in rats: the effect of injury severity and brain temperature.
      Authors: Vitarbo EA, Chatzipanteli K, Kinoshita K, Truettner JS, Alonso OF, Dietrich WD
      Neurosurgery, 2004;55(2):416-24; discu.
      Species: Rat
      Sample Type: Serum
    81. Oral (gavage), in utero and post-natal exposure of Sprague-Dawley rats to low doses of tributyltin chloride. Part II: effects on the immune system.
      Authors: Tryphonas H, Cooke G, Caldwell D, Bondy G, Parenteau M, Hayward S, Pulido O
      Food Chem. Toxicol., 2004;42(2):221-35.
      Species: Rat
      Sample Type: Serum
    82. Gene transfer of tumor necrosis factor inhibitor improves the function of lung allografts.
      Authors: Tagawa T, Kozower BD, Kanaan SA, Daddi N, Muraoka M, Oka T, Ritter JH, Patterson GA
      J. Thorac. Cardiovasc. Surg., 2004;127(6):1558-63.
      Species: Rat
      Sample Type: Tissue Homogenates
    83. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
      Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
      Endocrinology, 2004;145(9):4084-93.
      Species: Rat
      Sample Type: Tissue Homogenates
    84. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
      Authors: Schubert T, Horch RE
      Ann. Surg., 2004;240(2):340-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    85. Alveolar macrophage cytotoxicity for normal lung fibroblasts is mediated by nitric oxide release.
      Authors: Morgan DL, Shines CJ
      Toxicol In Vitro, 2004;18(1):139-46.
      Species: Rat
      Sample Type: Cell Culture Supernates
    86. Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
      Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H
      J. Physiol. (Lond.), 2004;556(0):193-202.
      Species: Rat
      Sample Type: Interstitial Fluid
    87. Anti-inflammatory effects of alpha-melanocyte-stimulating hormone against rat endotoxin-induced uveitis and the time course of inflammatory agents in aqueous humor.
      Authors: Nishida T, Miyata S, Itoh Y, Mizuki N, Ohgami K, Shiratori K, Ilieva IB, Ohno S, Taylor AW
      Int. Immunopharmacol., 2004;4(8):1059-66.
      Species: Rat
      Sample Type: Aqueous humor
    88. HIV-1 gp120 stimulates proinflammatory cytokine-mediated pain facilitation via activation of nitric oxide synthase-I (nNOS).
      Authors: Holguin A, O&amp;amp;apos;Connor KA, Biedenkapp J, Campisi J, Wieseler-Frank J, Milligan ED, Hansen MK, Spataro L, Maksimova E, Bravmann C, Martin D, Fleshner M, Maier SF, Watkins LR
      Pain, 2004;110(3):517-30.
      Species: Rat
      Sample Type: CSF
    89. Differential expression of cytokine genes and inducible nitric oxide synthase induced by opacity phenotype variants of Streptococcus pneumoniae during acute otitis media in the rat.
      Authors: Long JP, Tong HH, Shannon PA, DeMaria TF
      Infect. Immun., 2003;71(10):5531-40.
      Species: Rat
      Sample Type: Tissue Secretion
    90. Recipient intramuscular cotransfection of naked plasmid transforming growth factor beta1 and interleukin 10 ameliorates lung graft ischemia-reperfusion injury.
      Authors: D&amp;amp;apos;Ovidio F
      J. Thorac. Cardiovasc. Surg., 2002;124(2):259-69.
      Species: Rat
      Sample Type: BALF
    91. Differential effects of ebselen on neutrophil recruitment, chemokine, and inflammatory mediator expression in a rat model of lipopolysaccharide-induced pulmonary inflammation.
      Authors: Haddad el-B, McCluskie K, Birrell MA, Dabrowski D, Pecoraro M, Underwood S, Chen B, De Sanctis GT, Webber SE, Foster ML, Belvisi MG
      J. Immunol., 2002;169(2):974-82.
      Species: Rat
      Sample Type: BALF
    92. Iron intake increases infarct volume after permanent middle cerebral artery occlusion in rats.
      Authors: Castellanos M, Puig N, Carbonell T, Castillo J, Martinez J, Rama R, Davalos A
      Brain Res., 2002;952(1):1-6.
    93. Gene transfer of transforming growth factor-beta1 to the rat peritoneum: effects on membrane function.
      Authors: Margetts PJ, Kolb M, Galt T, Hoff CM, Shockley TR, Gauldie J
      J. Am. Soc. Nephrol., 2001;12(10):2029-39.
      Species: Rat
      Sample Type: Peritoneal Fluid
    Expand to show all 93 Citations
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Average Rating: 5 (Based on 1 review)

    Have you used Rat TNF-alpha Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Read what people are saying who have used Rat TNF-alpha Quantikine ELISA Kit.

    Filter by Ratings
    Excellent
    (1)
    All Ratings
    Filter by Applications
    All Applications
    Filter by Species
    All Species

    Images Ratings Applications Species Reviewed By Date Details
      Excellent
      Anonymous 10/20/2017
    Show

    Summary

    Sample TestedHeparin Plasma

    Order Details

    Contact Information

    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed

    Feedback

    Customer Information