Rat Vanilloid R1/TRPV1 Antibody

(2 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat Vanilloid R1/TRPV1 in immunohistochemistry.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    KLH-coupled rat Vanilloid R1/TRPV1 synthetic peptide
    RASLDSEESESPPQENSC
    Accession # O35433
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Vanilloid R1/TRPV1 in Rat Dorsal Root Ganglion. Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Goat Anti-Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
Vanilloid R1/TRPV1 in Rat Brain. Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat brain using Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vanilloid R1/TRPV1
VR1, also known as TRPV1 (Transient Receptor Potential Vanilloid 1) and capsaicin receptor, is a 115 kDa integral membrane ion channel that has six transmembrane domains and contains intracellular N-and C-termini. It contributes to normal pain and temperature sensation and also has a “sensory-effector” function. By alternative splicing, at least three VR1 isoforms are known. The sequence of residues 4-21 of rat VR1 is 78% and 28% identical to that of mouse and human VR1, respectively.
  • Long Name:
    Vanilloid Receptor 1/Transient Receptor Potential Cation Channel 1
  • Entrez Gene IDs:
    7442 (Human); 83810 (Rat)
  • Alternate Names:
    Capsaicin Receptor; Osm-9-like TRP channel 1; OTRPC1; transient receptor potential cation channel subfamily V member 1; transient receptor potential cation channel, subfamily V, member 1; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; TRPV1; Vanilloid R1; Vanilloid receptor 1; vanilloid receptor subtype 1; VanilloidR1; VR1; VR1DKFZp434K0220
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Mediation of movement-induced breakthrough cancer pain by IB4 binding nociceptors in rats
    Authors: J Havelin, I Imbert, D Sukhtankar, B Remeniuk, I Pelletier, J Gentry, A Okun, T Tiutan, F Porreca, T King
    J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  2. Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit.
    Authors: Isensee J, Wenzel C, Buschow R, Weissmann R, Kuss A, Hucho T
    PLoS ONE, 2014;9(12):e115731.
    Species: Rat
    Sample Type: Whole Cells
    Application: IHC Frozen
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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