Rat Vanilloid R1/TRPV1 Antibody

Catalog #: AF3066
4 Citations Datasheet / COA / SDS

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AF3066
AF3066-SP

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Vanilloid R1/TRPV1 in Rat Dorsal Root Ganglion.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Vanilloid R1/TRPV1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Vanilloid R1/TRPV1 in immunohistochemistry.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

KLH-coupled rat Vanilloid R1/TRPV1 synthetic peptide
RASLDSEESESPPQENSC
Accession # O35433

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Vanilloid R1/TRPV1 in Rat Dorsal Root Ganglion. Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Goat Anti-Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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Vanilloid R1/TRPV1 in Rat Brain. Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat brain using Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vanilloid R1/TRPV1

VR1, also known as TRPV1 (Transient Receptor Potential Vanilloid 1) and capsaicin receptor, is a 115 kDa integral membrane ion channel that has six transmembrane domains and contains intracellular N-and C-termini. It contributes to normal pain and temperature sensation and also has a “sensory-effector” function. By alternative splicing, at least three VR1 isoforms are known. The sequence of residues 4-21 of rat VR1 is 78% and 28% identical to that of mouse and human VR1, respectively.

Long Name

Vanilloid Receptor 1/Transient Receptor Potential Cation Channel 1

Entrez Gene IDs

7442 (Human); 193034 (Mouse); 83810 (Rat)

Alternate Names

Capsaicin Receptor; Osm-9-like TRP channel 1; OTRPC1; transient receptor potential cation channel subfamily V member 1; transient receptor potential cation channel, subfamily V, member 1; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; TRPV1; Vanilloid R1; Vanilloid receptor 1; vanilloid receptor subtype 1; VanilloidR1; VR1; VR1DKFZp434K0220

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Rat Vanilloid R1/TRPV1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Transient receptor potential melastatin-7 in the rat dorsal root ganglion
Authors: T Yajima, T Sato, H Hosokawa, T Kondo, H Ichikawa
Journal of chemical neuroanatomy, 2022;125(0):102163.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Transient receptor potential melastatin-3 in the rat sensory ganglia of the trigeminal, glossopharyngeal and vagus nerves
Authors: T Yajima, T Sato, K Shimazaki, H Ichikawa
J. Chem. Neuroanat., 2019;96(0):116-125.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Mediation of movement-induced breakthrough cancer pain by IB4 binding nociceptors in rats
Authors: J Havelin, I Imbert, D Sukhtankar, B Remeniuk, I Pelletier, J Gentry, A Okun, T Tiutan, F Porreca, T King
J. Neurosci., 2017;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit.
Authors: Isensee J, Wenzel C, Buschow R, Weissmann R, Kuss A, Hucho T
PLoS ONE, 2014;9(12):e115731.
Species: Rat
Sample Types: Whole Cells
Applications: IHC-Fr