Rat Vanilloid R1/TRPV1 Antibody Summary
Accession # O35433
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Vanilloid R1/TRPV1 in Rat Dorsal Root Ganglion. Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Goat Anti-Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Vanilloid R1/TRPV1 in Rat Brain. Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat brain using Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vanilloid R1/TRPV1
VR1, also known as TRPV1 (Transient Receptor Potential Vanilloid 1) and capsaicin receptor, is a 115 kDa integral membrane ion channel that has six transmembrane domains and contains intracellular N-and C-termini. It contributes to normal pain and temperature sensation and also has a “sensory-effector” function. By alternative splicing, at least three VR1 isoforms are known. The sequence of residues 4-21 of rat VR1 is 78% and 28% identical to that of mouse and human VR1, respectively.
Citations for Rat Vanilloid R1/TRPV1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Mediation of movement-induced breakthrough cancer pain by IB4 binding nociceptors in rats
Authors: J Havelin, I Imbert, D Sukhtankar, B Remeniuk, I Pelletier, J Gentry, A Okun, T Tiutan, F Porreca, T King
J. Neurosci., 2017;0(0):.
Sample Types: Whole Tissue
Applications: IHC OCT-embedded
Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit.
Authors: Isensee J, Wenzel C, Buschow R, Weissmann R, Kuss A, Hucho T
PLoS ONE, 2014;9(12):e115731.
Sample Types: Whole Cells
Applications: IHC Frozen
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