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Rat Vanilloid R1/TRPV1 Antibody

AF3066 3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3066
AF3066-SP
Vanilloid R1/TRPV1 in Rat Brain.
2 Images
Product Details
Citations (3)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Vanilloid R1/TRPV1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Vanilloid R1/TRPV1 in immunohistochemistry.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled rat Vanilloid R1/TRPV1 synthetic peptide
RASLDSEESESPPQENSC
Accession # O35433
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Vanilloid R1/TRPV1 antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

Vanilloid R1/TRPV1 in Rat Brain. Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat brain using Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Vanilloid R1/TRPV1 antibody in Rat Dorsal Root Ganglion by Immunohistochemistry (IHC-Fr). View Larger

Vanilloid R1/TRPV1 in Rat Dorsal Root Ganglion. Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Goat Anti-Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vanilloid R1/TRPV1

VR1, also known as TRPV1 (Transient Receptor Potential Vanilloid 1) and capsaicin receptor, is a 115 kDa integral membrane ion channel that has six transmembrane domains and contains intracellular N-and C-termini. It contributes to normal pain and temperature sensation and also has a “sensory-effector” function. By alternative splicing, at least three VR1 isoforms are known. The sequence of residues 4-21 of rat VR1 is 78% and 28% identical to that of mouse and human VR1, respectively.

Long Name
Vanilloid Receptor 1/Transient Receptor Potential Cation Channel 1
Entrez Gene IDs
7442 (Human); 193034 (Mouse); 83810 (Rat)
Alternate Names
Capsaicin Receptor; Osm-9-like TRP channel 1; OTRPC1; transient receptor potential cation channel subfamily V member 1; transient receptor potential cation channel, subfamily V, member 1; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; TRPV1; Vanilloid R1; Vanilloid receptor 1; vanilloid receptor subtype 1; VanilloidR1; VR1; VR1DKFZp434K0220

Product Datasheets

Product Datasheet
COA
SDS

Citations for Rat Vanilloid R1/TRPV1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Transient receptor potential melastatin-3 in the rat sensory ganglia of the trigeminal, glossopharyngeal and vagus nerves
    Authors: T Yajima, T Sato, K Shimazaki, H Ichikawa
    J. Chem. Neuroanat., 2019;96(0):116-125.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Mediation of movement-induced breakthrough cancer pain by IB4 binding nociceptors in rats
    Authors: J Havelin, I Imbert, D Sukhtankar, B Remeniuk, I Pelletier, J Gentry, A Okun, T Tiutan, F Porreca, T King
    J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit.
    Authors: Isensee J, Wenzel C, Buschow R, Weissmann R, Kuss A, Hucho T
    PLoS ONE, 2014;9(12):e115731.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC-Fr

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