Recombinant Human BDNF Protein

100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined
  (94 citations)
(6 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (94)
FAQs
Reviews
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL.
    The specific activity of Recombinant Human/Mouse/Rat/Canine/Equine BDNF is approximately 1.3 x 103 units/μg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534).
    Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) is coated at 1 μg/mL, Recombinant Human BDNF binds with an apparent Kd <1 nM.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived human BDNF protein
    His129-Arg247
  • Accession #
  • N-terminal Sequence
    Analysis
    His129
  • Predicted Molecular Mass
    13.5 kDa
  • SDS-PAGE
    13-14 kDa, reducing conditions
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
248-BD
 
248-BD/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human BDNF (Catalog #
248‑BD) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL.
1 μg/lane of Recombinant Human BDNF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins (1, 2). All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor (3).

  • References:
    1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
    2. Snider, W.D. (1994) Cell 77:627.
    3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
  • Long Name:
    Brain-derived Neurotrophic Factor
  • Entrez Gene IDs:
    627 (Human); 12064 (Mouse); 24225 (Rat)
  • Alternate Names:
    Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

94 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. DNA topoisomerase II? stimulates neurite outgrowth in neural differentiated human mesenchymal stem cells through regulation of Rho-GTPases (RhoA/Rock2 pathway) and Nurr1 expression
    Authors: M Zaim, S Isik
    Stem Cell Res Ther, 2018;9(1):114.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Dissecting the Functional Consequences of De Novo DNA Methylation Dynamics in Human Motor Neuron Differentiation and Physiology
    Authors: MJ Ziller, JA Ortega, KA Quinlan, DP Santos, H Gu, EJ Martin, C Galonska, R Pop, S Maidl, A Di Pardo, M Huang, HY Meltzer, A Gnirke, CJ Heckman, A Meissner, E Kiskinis
    Cell Stem Cell, 2018;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Integrative proteomic and transcriptomic analysis provides evidence for TrkB (NTRK2) as a therapeutic target in combination with tyrosine kinase inhibitors for non-small cell lung cancer
    Authors: DR Gomez, LA Byers, M Nilsson, L Diao, J Wang, L Li, P Tong, M Hofstad, B Saigal, I Wistuba, N Kalhor, S Swisher, Y Fan, WK Hong, M Suraokar, C Behrens, C Moran, JV Heymach
    Oncotarget, 2018;9(18):14268-14284.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Subcellular localisation modulates ubiquitylation and degradation of Ascl1
    Authors: S Gillotin, JD Davies, A Philpott
    Sci Rep, 2018;8(1):4625.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells
    Authors: G Inana, C Murat, W An, X Yao, IR Harris, J Cao
    J Transl Med, 2018;16(1):63.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease
    Authors: M Chumarina, C Azevedo, J Bigarreau, C Vignon, KS Kim, JY Li, L Roybon
    Stem Cell Res, 2017;19(0):17-20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
    Authors: X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky
    PLoS ONE, 2017;12(4):e0174074.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Overexpression of matrix metalloproteinase-9 (MMP-9) rescues insulin-mediated impairment in the 5XFAD model of Alzheimer's disease
    Authors: A Kaminari, N Giannakas, A Tzinia, EC Tsilibary
    Sci Rep, 2017;7(1):683.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Presynaptic morphology and vesicular composition determine vesicle dynamics in mouse central synapses
    Authors: L Guillaud, D Dimitrov, T Takahashi
    Elife, 2017;6(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
    Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Differentiation of nestin?negative human hair follicle outer root sheath cells into neurons in�vitro
    Authors: W Wu, XL Wu, YQ Ji, Z Gao
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. EndophilinAs regulate endosomal sorting of BDNF-TrkB to mediate survival signaling in hippocampal neurons
    Authors: K Burk, JD Murdoch, S Freytag, M Koenig, V Bharat, R Markworth, S Burkhardt, A Fischer, C Dean
    Sci Rep, 2017;7(1):2149.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke
    Authors: KY Tseng, JE Anttila, K Khodosevic, RK Tuominen, M Lindahl, A Domanskyi, M Airavaara
    Mol. Ther., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Alternative NHEJ pathway proteins as components of MYCN oncogenic activity in human neural crest stem cell differentiation: implications for neuroblastoma initiation
    Authors: EA Newman, S Chukkapall, D Bashllari, TT Thomas, RA Van Noord, ER Lawlor, MJ Hoenerhoff, AW Opipari, VP Opipari
    Cell Death Dis, 2017;8(12):3208.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Common genetic variation drives molecular heterogeneity in human iPSCs
    Authors: H Kilpinen, A Goncalves, A Leha, V Afzal, K Alasoo, S Ashford, S Bala, D Bensaddek, FP Casale, OJ Culley, P Danecek, A Faulconbri, PW Harrison, A Kathuria, D McCarthy, SA McCarthy, R Meleckyte, Y Memari, N Moens, F Soares, A Mann, I Streeter, CA Agu, A Alderton, R Nelson, S Harper, M Patel, A White, SR Patel, L Clarke, R Halai, CM Kirton, A Kolb-Kokoc, P Beales, E Birney, D Danovi, AI Lamond, WH Ouwehand, L Vallier, FM Watt, R Durbin, O Stegle, DJ Gaffney
    Nature, 2017;546(7658):370-375.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Whole genome microarray analysis of neural progenitor C17.2 cells during differentiation and validation of 30 neural mRNA biomarkers for estimation of developmental neurotoxicity
    Authors: K Attoff, A Gliga, J Lundqvist, U Norinder, A Forsby
    PLoS ONE, 2017;12(12):e0190066.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Neurotoxic reactive astrocytes are induced by activated microglia
    Authors: SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres
    Nature, 2017;541(7638):481-487.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells
    Authors: T Yokoi, T Tanaka, E Matsuzaka, F Tamalu, SI Watanabe, S Nishina, N Azuma
    Sci Rep, 2017;7(1):16757.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Altered Differentiation Potential of Gaucher's Disease iPSC Neuronal Progenitors due to Wnt/?-Catenin Downregulation
    Authors: O Awad, LM Panicker, RM Deranieh, MP Srikanth, RA Brown, A Voit, T Peesay, TS Park, ET Zambidis, RA Feldman
    Stem Cell Reports, 2017;9(6):1853-1867.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Axonal Type III Nrg1 Controls Glutamate Synapse Formation and GluA2 Trafficking in Hippocampal-Accumbens Connections
    Authors: C Zhong, W Akmentin, C Du, LW Role, DA Talmage
    eNeuro, 2017;4(1):.
    Species: Mouse
    Sample Type: Complex Sample Type
    Application: Bioassay
  21. Differential gene expression profiles in neurons generated from lymphoblastoid B-cell line-derived iPS cells from monozygotic twin cases with treatment-resistant schizophrenia and discordant responses to clozapine
    Authors: T Nakazawa, M Kikuchi, M Ishikawa, H Yamamori, K Nagayasu, T Matsumoto, M Fujimoto, Y Yasuda, M Fujiwara, S Okada, K Matsumura, A Kasai, A Hayata-Tak, N Shintani, S Numata, K Takuma, W Akamatsu, H Okano, A Nakaya, H Hashimoto, R Hashimoto
    Schizophr. Res., 2017;181(0):75-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Three-tier regulation of cell number plasticity by neurotrophins and Tolls in Drosophila
    Authors: I Foldi, N Anthoney, N Harrison, M Gangloff, B Verstak, MP Nallasivan, S AlAhmed, B Zhu, M Phizacklea, M Losada-Per, M Moreira, NJ Gay, A Hidalgo
    J. Cell Biol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins
    Authors: SP Jenkinson, D Grandgirar, M Heidemann, A Tscherter, MA Avondet, SL Leib
    Front Pharmacol, 2017;8(0):73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells
    Authors: Y Qi, XJ Zhang, N Renier, Z Wu, T Atkin, Z Sun, MZ Ozair, J Tchieu, B Zimmer, F Fattahi, Y Ganat, R Azevedo, N Zeltner, AH Brivanlou, M Karayiorgo, J Gogos, M Tomishima, M Tessier-La, SH Shi, L Studer
    Nat. Biotechnol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016;6(0):31063.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia
    Authors: N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer
    Nat. Med., 2016;22(12):1421-1427.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Induced pluripotent stem cells (iPSC) created from skin fibroblasts of patients with Prader-Willi syndrome (PWS) retain the molecular signature of PWS
    Stem Cell Res, 2016;17(3):526-530.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs)
    Authors: Divya Varun
    Acta Biomater, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Sustained synchronized neuronal network activity in a human astrocyte co-culture system
    Sci Rep, 2016;6(0):36529.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. Neurotrophin-3 Regulates Synapse Development by Modulating TrkC-PTP? Synaptic Adhesion and Intracellular Signaling Pathways
    J Neurosci, 2016;36(17):4816-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Dopamine Receptor Antagonists Enhance Proliferation and Neurogenesis of Midbrain Lmx1a-expressing Progenitors
    Authors: Eva Hedlund
    Sci Rep, 2016;6(0):26448.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  32. Hypomorphism of Fto and Rpgrip1l causes obesity in mice
    Authors: G Stratigopo, LC Burnett, R Rausch, R Gill, DB Penn, AA Skowronski, CA LeDuc, AJ Lanzano, P Zhang, DR Storm, D Egli, RL Leibel
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  33. Effects of Feeder Cells on Dopaminergic Differentiation of Human Embryonic Stem Cells
    Authors: Z Zhao, Y Ma, Z Chen, Q Liu, Q Li, D Kong, K Yuan, L Hu, T Wang, X Chen, Y Peng, W Jiang, Y Yu, X Liu
    Front Cell Neurosci, 2016;10(0):291.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells
    Sci Rep, 2016;6(0):32532.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. Increased susceptibility to a? toxicity in neuronal cultures derived from familial Azheimer's disease (PSEN1-A246E) induced pluripotent stem cells
    Authors: Enrique Armijo
    Neurosci. Lett, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.
    Authors: Lim M, Shin M, Lee S, Minn Y, Hoh J, Cho Y, Kim D, Lee S, Kim C, Park C
    PLoS ONE, 2015;10(9):e0138460.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  37. Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability.
    Authors: Devlin, Anna-Cla, Burr, Karen, Borooah, Shyamang, Foster, Joshua D, Cleary, Elaine M, Geti, Imbisaat, Vallier, Ludovic, Shaw, Christop, Chandran, Siddhart, Miles, Gareth B
    Nat Commun, 2015;6(0):5999.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  38. Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in Human Pulmonary Artery Smooth Muscle.
    Authors: Hartman W, Helan M, Smelter D, Sathish V, Thompson M, Pabelick C, Johnson B, Prakash Y
    PLoS ONE, 2015;10(7):e0129489.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  39. Neuropeptide Y receptor Y5 as an inducible pro-survival factor in neuroblastoma: implications for tumor chemoresistance.
    Authors: Czarnecka M, Trinh E, Lu C, Kuan-Celarier A, Galli S, Hong S, Tilan J, Talisman N, Izycka-Swieszewska E, Tsuei J, Yang C, Martin S, Horton M, Christian D, Everhart L, Maheswaran I, Kitlinska J
    Oncogene, 2015;34(24):3131-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  40. Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinson's disease.
    Authors: Laguna A, Schintu N, Nobre A, Alvarsson A, Volakakis N, Jacobsen J, Gomez-Galan M, Sopova E, Joodmardi E, Yoshitake T, Deng Q, Kehr J, Ericson J, Svenningsson P, Shupliakov O, Perlmann T
    Nat Neurosci, 2015;18(6):826-35.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  41. Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells.
    Authors: Merkle F, Maroof A, Wataya T, Sasai Y, Studer L, Eggan K, Schier A
    Development, 2015;142(4):633-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  42. Neuroprotective effects of oligodendrocyte progenitor cell transplantation in premature rat brain following hypoxic-ischemic injury.
    Authors: Chen, Long-Xia, Ma, Si-Min, Zhang, Peng, Fan, Zi-Chuan, Xiong, Man, Cheng, Guo-Qian, Yang, Yi, Qiu, Zi-Long, Zhou, Wen-Hao, Li, Jin
    PLoS ONE, 2015;10(3):e0115997.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  43. Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts.
    Authors: Wainger B, Buttermore E, Oliveira J, Mellin C, Lee S, Saber W, Wang A, Ichida J, Chiu I, Barrett L, Huebner E, Bilgin C, Tsujimoto N, Brenneis C, Kapur K, Rubin L, Eggan K, Woolf C
    Nat Neurosci, 2015;18(1):17-24.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  44. Modeling the Early Phenotype at the Neuromuscular Junction of Spinal Muscular Atrophy Using Patient-Derived iPSCs.
    Authors: Yoshida M, Kitaoka S, Egawa N, Yamane M, Ikeda R, Tsukita K, Amano N, Watanabe A, Morimoto M, Takahashi J, Hosoi H, Nakahata T, Inoue H, Saito M
    Stem Cell Reports, 2015;4(4):561-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  45. Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells.
    Authors: Tanaka, Taku, Yokoi, Tadashi, Tamalu, Fuminobu, Watanabe, Shu-Ichi, Nishina, Sachiko, Azuma, Noriyuki
    Sci Rep, 2015;5(0):8344.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  46. Generation of Dopamine Neurons from Rodent Fibroblasts through the Expandable Neural Precursor Cell Stage.
    Authors: Lim M, Chang M, Kim S, Yi S, Suh-Kim H, Jung S, Kim M, Kim J, Lee Y, Lee S, Kim D, Lee S, Park C
    J Biol Chem, 2015;290(28):17401-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  47. Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling.
    Authors: Chang, Ki-Young, Woo, Doyeon, Jung, Hyunjin, Lee, Sangkyu, Kim, Sungsoo, Won, Joungha, Kyung, Taeyoon, Park, Hyerim, Kim, Nury, Yang, Hee Won, Park, Jae-Yong, Hwang, Eun Mi, Kim, Daesoo, Heo, Won Do
    Nat Commun, 2014;5(0):4057.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  48. Hybrid multiphoton volumetric functional imaging of large-scale bioengineered neuronal networks.
    Authors: Dana, Hod, Marom, Anat, Paluch, Shir, Dvorkin, Roman, Brosh, Inbar, Shoham, Shy
    Nat Commun, 2014;5(0):3997.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  49. Highly efficient generation of induced neurons from human fibroblasts that survive transplantation into the adult rat brain.
    Authors: Pereira, Maria, Pfisterer, Ulrich, Rylander, Daniella, Torper, Olof, Lau, Shong, Lundblad, Martin, Grealish, Shane, Parmar, Malin
    Sci Rep, 2014;4(0):6330.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  50. Reprogramming non-human primate somatic cells into functional neuronal cells by defined factors.
    Authors: Zhou, Zhi, Kohda, Kazuhisa, Ibata, Keiji, Kohyama, Jun, Akamatsu, Wado, Yuzaki, Michisuk, Okano, Hirotaka, Sasaki, Erika, Okano, Hideyuki
    Mol Brain, 2014;7(0):24.
    Species: Primate - Callitrix jacchus (Common Marmoset)
    Sample Type: Whole Cells
    Application: Bioassay
  51. Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells.
    Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris
    PLoS ONE, 2014;9(12):e116114.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  52. Infralimbic BDNF/TrkB enhancement of GluN2B currents facilitates extinction of a cocaine-conditioned place preference.
    Authors: Otis J, Fitzgerald M, Mueller D
    J Neurosci, 2014;34(17):6057-64.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  53. Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors.
    Authors: Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G
    EMBO J, 2014;33(14):1582-98.
    Species: N/A
    Sample Type: Protein
    Application: WB Standard
  54. Long-term culture of rat hippocampal neurons at low density in serum-free medium: combination of the sandwich culture technique with the three-dimensional nanofibrous hydrogel PuraMatrix.
    Authors: Kaneko A, Sankai Y
    PLoS ONE, 2014;9(7):e102703.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Bioassay
  55. Local translation and retrograde axonal transport of CREB regulates IL-6-induced nociceptive plasticity.
    Authors: Melemedjian O, Tillu D, Moy J, Asiedu M, Mandell E, Ghosh S, Dussor G, Price T
    Mol Pain, 2014;10(0):45.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  56. A time course analysis of the electrophysiological properties of neurons differentiated from human induced pluripotent stem cells (iPSCs).
    Authors: Pre D, Nestor M, Sproul A, Jacob S, Koppensteiner P, Chinchalongporn V, Zimmer M, Yamamoto A, Noggle S, Arancio O
    PLoS ONE, 2014;9(7):e103418.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  57. Constitutively active TrkB confers an aggressive transformed phenotype to a neural crest-derived cell line.
    Authors: Dewitt J, Ochoa V, Urschitz J, Elston M, Moisyadi S, Nishi R
    Oncogene, 2014;33(8):977-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  58. Self-assembling peptide nanofiber scaffolds enhance dopaminergic differentiation of mouse pluripotent stem cells in 3-dimensional culture.
    Authors: Ni, Na, Hu, Yaohua, Ren, Huixia, Luo, Chuanmin, Li, Peng, Wan, Jian-Bo, Su, Huanxing
    PLoS ONE, 2013;8(12):e84504.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  59. Inhibition of PIKfyve by YM-201636 dysregulates autophagy and leads to apoptosis-independent neuronal cell death.
    Authors: Martin, Sally, Harper, Callista, May, Linda M, Coulson, Elizabet, Meunier, Frederic, Osborne, Shona L
    PLoS ONE, 2013;8(3):e60152.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  60. A small molecule screen in stem-cell-derived motor neurons identifies a kinase inhibitor as a candidate therapeutic for ALS.
    Authors: Yang Y, Gupta S, Kim K, Powers B, Cerqueira A, Wainger B, Ngo H, Rosowski K, Schein P, Ackeifi C, Arvanites A, Davidow L, Woolf C, Rubin L
    Cell Stem Cell, 2013;12(6):713-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  61. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  62. Enrichment and characterization of human dermal stem/progenitor cells by intracellular granularity.
    Authors: Shim, Joong Hy, Lee, Tae Ryon, Shin, Dong Woo
    Stem Cells Dev, 2013;22(8):1264-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  63. Nicotine elicits prolonged calcium signaling along ventral hippocampal axons.
    Authors: Zhong C, Talmage D, Role L
    PLoS ONE, 2013;8(12):e82719.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  64. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013;31(5):426-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  65. ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75NTR-dependent mechanism.
    Authors: Howard, Laura, Wyatt, Sean, Nagappan, Guhan, Davies, Alun M
    Development, 2013;140(10):2108-17.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  66. Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits.
    Authors: Liu Y, Weick J, Liu H, Krencik R, Zhang X, Ma L, Zhou G, Ayala M, Zhang S
    Nat Biotechnol, 2013;31(5):440-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  67. MicroRNA-based promotion of human neuronal differentiation and subtype specification.
    Authors: Stappert L, Borghese L, Roese-Koerner B, Weinhold S, Koch P, Terstegge S, Uhrberg M, Wernet P, Brustle O
    PLoS ONE, 2013;8(3):e59011.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  68. NeuroD1 regulates survival and migration of neuroendocrine lung carcinomas via signaling molecules TrkB and NCAM.
    Authors: Osborne, Jihan K, Larsen, Jill E, Shields, Misty D, Gonzales, Joshua X, Shames, David S, Sato, Mitsuo, Kulkarni, Ashwinik, Wistuba, Ignacio, Girard, Luc, Minna, John D, Cobb, Melanie
    Proc Natl Acad Sci U S A, 2013;110(16):6524-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  69. A human Dravet syndrome model from patient induced pluripotent stem cells.
    Authors: Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, Imaizumi Y, Zhang B, Nabeshima K, Mori M, Katsurabayashi S, Shirasaka Y, Okano H, Hirose S
    Mol Brain, 2013;6(0):19.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  70. mTOR-dependent proliferation defect in human ES-derived neural stem cells affected by myotonic dystrophy type 1.
    Authors: Denis J, Gauthier M, Rachdi L, Aubert S, Giraud-Triboult K, Poydenot P, Benchoua A, Champon B, Maury Y, Baldeschi C, Scharfmann R, Pietu G, Peschanski M, Martinat C
    J Cell Sci, 2013;126(0):1763-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  71. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells.
    Authors: Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, Liu B, Goulburn A, Stanley E, Elefanty A, Widmer H, Eggan K, Goldstein P, Anderson S, Studer L
    Cell Stem Cell, 2013;12(5):559-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  72. Sodium phenylbutyrate enhances astrocytic neurotrophin synthesis via protein kinase C (PKC)-mediated activation of cAMP-response element-binding protein (CREB): implications for Alzheimer disease therapy.
    Authors: Corbett, Grant T, Roy, Avik, Pahan, Kalipada
    J Biol Chem, 2013;288(12):8299-312.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  73. Neuregulin and BDNF induce a switch to NMDA receptor-dependent myelination by oligodendrocytes.
    Authors: Lundgaard I, Luzhynskaya A, Stockley J, Wang Z, Evans K, Swire M, Volbracht K, Gautier H, Franklin R, Attwell D, Karadottir R
    PLoS Biol, 2013;11(12):e1001743.
    Species: Rat
    Sample Type: Whole Cells
    Application: cell culture
  74. BDNF regulates atypical PKC at spinal synapses to initiate and maintain a centralized chronic pain state.
    Authors: Melemedjian O, Tillu D, Asiedu M, Mandell E, Moy J, Blute V, Taylor C, Ghosh S, Price T
    Mol Pain, 2013;9(0):12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  75. E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
    Authors: Moore RN, Cherry JF, Mathur V
    Stem Cells Dev., 2012;21(1):30-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  76. Inhibition of uterine sarcoma cell growth through suppression of endogenous tyrosine kinase B signaling.
    Authors: Makino, Kenichi, Kawamura, Kazuhiro, Sato, Wataru, Kawamura, Nanami, Fujimoto, Toshio, Terada, Yukihiro
    PLoS ONE, 2012;7(7):e41049.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  77. The suppressive effect of an intra-prefrontal cortical infusion of BDNF on cocaine-seeking is Trk receptor and extracellular signal-regulated protein kinase mitogen-activated protein kinase dependent.
    Authors: Whitfield TW, Shi X, Sun WL
    J. Neurosci., 2011;31(3):834-42.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  78. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Surface Plasmon Resonance
  79. Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1.
    Authors: Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, D'Hooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chedotal A, Honnorat J, Thomasset N, Carmeliet P, Meissirel C
    J. Neurosci., 2010;30(45):15052-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  80. EGF and bFGF pre-treatment enhances neural specification and the response to neuronal commitment of MIAMI cells.
    Authors: Delcroix GJ, Curtis KM, Schiller PC, Montero-Menei CN
    Differentiation, 2010;80(4):213-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  81. A single intra-PFC infusion of BDNF prevents cocaine-induced alterations in extracellular glutamate within the nucleus accumbens.
    Authors: Berglind WJ, Whitfield TW, LaLumiere RT, Kalivas PW, McGinty JF
    J. Neurosci., 2009;29(12):3715-9.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  82. Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture.
    Authors: Sun Y, Pollard S, Conti L, Toselli M, Biella G, Parkin G, Willatt L, Falk A, Cattaneo E, Smith A
    Mol. Cell. Neurosci., 2008;38(2):245-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  83. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
    Authors: Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L
    Genes Dev., 2008;22(2):152-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  84. Brain-derived neurotrophic factor stimulates bone/cementum-related protein gene expression in cementoblasts.
    Authors: Kajiya M, Shiba H, Fujita T, Ouhara K, Takeda K, Mizuno N, Kawaguchi H, Kitagawa M, Takata T, Tsuji K, Kurihara H
    J. Biol. Chem., 2008;283(23):16259-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  85. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
    Authors: Samah B, Porcheray F, Gras G
    Clin. Exp. Immunol., 2008;151(3):476-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  86. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(8):2997-3002.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  87. SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
    Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
    Development, 2007;134(18):3327-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  88. Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat.
    Authors: Paredes D, Granholm AC, Bickford PC
    Brain Res., 2007;1141(0):56-64.
  89. A BDNF infusion into the medial prefrontal cortex suppresses cocaine seeking in rats.
    Authors: Berglind WJ, See RE, Fuchs RA, Ghee SM, Whitfield TW, Miller SW, McGinty JF
    Eur. J. Neurosci., 2007;26(3):757-66.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  90. A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin.
    Authors: Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S
    Stem Cells, 2007;26(1):163-72.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  91. BDNF plasma levels in acute stroke.
    Authors: Di Lazzaro V, Profice P, Pilato F, Dileone M, Florio L, Tonali PA, Angelucci F
    Neurosci. Lett., 2007;422(2):128-30.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  92. Multipotent adult progenitor cells from swine bone marrow.
    Authors: Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, O'Brien TD, Zhang J, Verfaillie C
    Stem Cells, 2006;24(11):2355-66.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Bioassay
  93. Platelet-derived growth factor (PDGF-BB) and brain-derived neurotrophic factor (BDNF) induce striatal neurogenesis in adult rats with 6-hydroxydopamine lesions.
    Authors: Mohapel P, Frielingsdorf H, Haggblad J, Zachrisson O, Brundin P
    Neuroscience, 2005;132(3):767-76.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  94. Derivation of midbrain dopamine neurons from human embryonic stem cells.
    Authors: Perrier AL, Tabar V, Barberi T, Rubio ME, Bruses J, Topf N, Harrison NL, Studer L
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(34):12543-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
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Average Rating: 5 (Based on 6 reviews)

Have you used Recombinant Human BDNF Protein?

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Read what people are saying who have used Recombinant Human BDNF Protein.

We have 6 reviews tested in 5 applications: In vitro bioactivity in cell culture, Immunoassay Standard, Stem/Immune cell maintenance or differentiation , Cell proliferation, In vivo study.

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In vitro bioactivity in cell culture Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein 248-BD Array 248-BD
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Excellent
 In vitro bioactivity in cell culture Anonymous 12/01/2017
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In vitro bioactivity in cell culture Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein 248-BD
In vitro bioactivity in cell culture: Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein [248-BD].

Summary

Application In vitro bioactivity in cell culture
Reason for Rating fast and product is very potent
  Excellent
 Immunoassay Standard Anonymous 11/02/2017
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Summary

Application Immunoassay Standard

Other Experimental Details

Other Experimental Details The recombinant BDNF (248-BD) was used as the standard for an ELISA assay. It worked exactly as expected, enabling the quantification of human serum and plasma samples.
  Excellent
 Stem/Immune cell maintenance or differentiation Anonymous 05/22/2017
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Summary

Application Stem/Immune cell maintenance or differentiation
Reason for Rating Worked well with iPSCs as well as NSCs derived neuronal maturation.
  Excellent
 Cell proliferation Anonymous 05/10/2017
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Summary

Application Cell proliferation
  Excellent
 Stem/Immune cell maintenance or differentiation Anonymous 12/15/2016
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Summary

Application Stem/Immune cell maintenance or differentiation

Other Experimental Details

Other Experimental Details This reagent has been used for culture of human neural stem cells (5 ug/ml) and differentiation into cortical neurons (25 ug/ml).
In vivo study Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein 248-BD Array 248-BD
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Excellent
 In vivo study Anonymous 05/12/2016
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In vivo study Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein 248-BD
In vivo study: Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein [248-BD].

Summary

Application In vivo study
Reason for Rating I did not receive positive data so I cannot say that it worked for sure.

Other Experimental Details

Other Experimental Details testing on affecting nicotine self-administration

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