Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

Simple Plex Human IL-6 2nd-gen assay kit for use on Ella automated benchtop ELISA. Contains cartridge, sample diluent SD13, and wash buffer
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Human IL-6 Simple Plex Assay
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Simple Plex Human IL-6 (2nd Gen) Assay Cartridge Summary

Sample Type & Volume Required Per Well*
Bronchoalveolar Lavage Fluid (12500 nL), Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (12500 nL)
0.28 pg/mL
Assay Range
0.3 - 2,652 pg/mL (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Natural and recombinant human IL-6.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-6 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), bronchoalveolar lavage fluid (BALF), and cerebrospinal fluid (CSF) on Ella automated benchtop ELISA. 


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 37.9 1763 38.7 1709
Standard Deviation 0.804 108 3.95 163
CV% 2.1 6.1 10.2 9.6


Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=6) 95 92-98
Heparin Plasma (n=6) 93 91-94
Serum (n=6) 94 86-98


Samples containing and/or spiked with high concentrations of human IL-6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the IL-6 assay. This automated IL-6 ELISA is validated to measure IL-6 in cell culture supernates (CCS), serum, plasma, bronchoalveolar lavage fluid (BALF), and cerebrospinal fluid (CSF).
Human IL-6 Simple Plex Assay Linearity

Scientific Data

Human IL-6 Simple Plex Assay Standard Curve

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

Long Name:
Interleukin 6
Entrez Gene IDs:
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names:
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Citation for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Torquetenovirus in pregnancy: Correlation with vaginal microbiome, metabolome and pro-inflammatory cytokines
    Authors: S Morselli, C Foschi, L Laghi, S Zagonari, G Patuelli, T Camboni, C Ceccarani, C Consolandi, ME Djusse, MF Pedna, A Marangoni, M Severgnini, V Sambri
    Frontiers in Microbiology, 2022;13(0):998849.
    Species: Human
    Sample Types: Cell Culture Supernatants


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